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Microbial Ecology

, Volume 63, Issue 3, pp 496–508 | Cite as

Dissolved Organic Carbon as Major Environmental Factor Affecting Bacterioplankton Communities in Mountain Lakes of Eastern Japan

  • Masanori Fujii
  • Hisaya Kojima
  • Tomoya Iwata
  • Jotaro Urabe
  • Manabu Fukui
Environmental Microbiology

Abstract

Relationships between environmental factors and bacterial communities were investigated in 41 freshwater lakes located in mountainous regions of eastern Japan. Bacterioplankton community composition (BCC) was determined by polymerase chain reaction-denaturing gradient gel electrophoresis of the 16S rRNA gene and then evaluated on the basis of physicochemical and biological variables of the lakes. Canonical correspondence analysis revealed that BCC of oligotrophic lakes was significantly influenced by dissolved organic carbon (DOC) content, but its effect was not apparent in the analysis covering all lakes including mesotrophic and eutrophic ones. The generalized linear model showed the negative association of DOC on the taxon richness of bacterioplankton communities. DOC was positively correlated with the catchment area per lake volume, suggesting that a large fraction of DOC supplied to the lake was derived from terrestrial sources. These results suggest that allochthonous DOC has a significant effect on bacterioplankton communities especially in oligotrophic lakes. The genus Polynucleobacter was detected most frequently. The occurrence of Polynucleobacter species was positively associated with DOC and negatively associated with total phosphorus (TP) levels. In addition, TP had a stronger effect than DOC, suggesting that oligotrophy is the most important factor on the occurrence of this genus.

Keywords

Total Phosphorus Canonical Correspondence Analysis Dissolve Organic Carbon Concentration Taxon Richness Freshwater Environment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We thank T. Hirao for his critical advice and technical assistance in statistical analysis. We also thank T. Suzuki, R. Yagami, K. Hosaka, and K. Suematsu for their field assistance and for providing data of environmental parameters. This study was supported by a grant in aid from the Ministry of Environment for the Global Environment Research Fund (F-052) and was partially supported by a grant from the Ministry of Education, Culture, Sports, Science and Technology, Japan, to Fukui (22370005).

Supplementary material

248_2011_9983_MOESM1_ESM.xls (66 kb)
ESM 1 (XLS 66 kb)

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Masanori Fujii
    • 1
  • Hisaya Kojima
    • 1
  • Tomoya Iwata
    • 2
  • Jotaro Urabe
    • 3
  • Manabu Fukui
    • 1
  1. 1.The Institute of Low Temperature ScienceHokkaido UniversitySapporoJapan
  2. 2.Department of Ecosocial System EngineeringUniversity of YamanashiKofuJapan
  3. 3.Division of Ecology and Evolutionary Biology, School of Life SciencesTohoku UniversitySendaiJapan

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