Microbial Ecology

, Volume 62, Issue 4, pp 824–837 | Cite as

Taxonomic and Functional Metagenomic Profiling of the Microbial Community in the Anoxic Sediment of a Sub-saline Shallow Lake (Laguna de Carrizo, Central Spain)

  • Manuel Ferrer
  • María-Eugenia Guazzaroni
  • Michael Richter
  • Adela García-Salamanca
  • Pablo Yarza
  • Ana Suárez-Suárez
  • Jennifer Solano
  • María Alcaide
  • Pieter van Dillewijn
  • Maria Antonia Molina-Henares
  • Nieves López-Cortés
  • Yamal Al-Ramahi
  • Carmen Guerrero
  • Alejandro Acosta
  • Laura I. de Eugenio
  • Virginia Martínez
  • Silvia Marques
  • Fernando Rojo
  • Eduardo Santero
  • Olga Genilloud
  • Julian Pérez-Pérez
  • Ramón Rosselló-Móra
  • Juan Luis Ramos
Environmental Microbiology

Abstract

The phylogenetic and functional structure of the microbial community residing in a Ca2+-rich anoxic sediment of a sub-saline shallow lake (Laguna de Carrizo, initially operated as a gypsum (CaSO4 × 2 H2O) mine) was estimated by analyzing the diversity of 16S rRNA amplicons and a 3.1 Mb of consensus metagenome sequence. The lake has about half the salinity of seawater and possesses an unusual relative concentration of ions, with Ca2+ and SO42- being dominant. The 16S rRNA sequences revealed a diverse community with about 22% of the bacterial rRNAs being less than 94.5% similar to any rRNA currently deposited in GenBank. In addition to this, about 79% of the archaeal rRNA genes were mostly related to uncultured Euryarchaeota of the CCA47 group, which are often associated with marine and oxygen-depleted sites. Sequence analysis of assembled genes revealed that 23% of the open reading frames of the metagenome library had no hits in the database. Among annotated genes, functions related to (thio) sulfate and (thio) sulfonate-reduction and iron-oxidation, sulfur-oxidation, denitrification, synthrophism, and phototrophic sulfur metabolism were found as predominant. Phylogenetic and biochemical analyses indicate that the inherent physical–chemical characteristics of this habitat coupled with adaptation to anthropogenic activities have resulted in a highly efficient community for the assimilation of polysulfides, sulfoxides, and organosulfonates together with nitro-, nitrile-, and cyanide-substituted compounds. We discuss that the relevant microbial composition and metabolic capacities at Laguna de Carrizo, likely developed as an adaptation to thrive in the presence of moderate salinity conditions and potential toxic bio-molecules, in contrast with the properties of previously known anoxic sediments of shallow lakes.

Supplementary material

248_2011_9903_MOESM1_ESM.pdf (6.8 mb)
ESM 1(PDF 6927 kb)

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Manuel Ferrer
    • 1
  • María-Eugenia Guazzaroni
    • 1
  • Michael Richter
    • 2
  • Adela García-Salamanca
    • 3
  • Pablo Yarza
    • 2
  • Ana Suárez-Suárez
    • 2
  • Jennifer Solano
    • 4
  • María Alcaide
    • 1
  • Pieter van Dillewijn
    • 3
  • Maria Antonia Molina-Henares
    • 3
  • Nieves López-Cortés
    • 1
  • Yamal Al-Ramahi
    • 1
  • Carmen Guerrero
    • 5
  • Alejandro Acosta
    • 3
  • Laura I. de Eugenio
    • 6
  • Virginia Martínez
    • 6
  • Silvia Marques
    • 3
  • Fernando Rojo
    • 7
  • Eduardo Santero
    • 8
  • Olga Genilloud
    • 9
  • Julian Pérez-Pérez
    • 10
  • Ramón Rosselló-Móra
    • 2
  • Juan Luis Ramos
    • 3
  1. 1.CSIC, Institute of CatalysisMadridSpain
  2. 2.IMEDEA (CSIC-UIB)EsporlesSpain
  3. 3.CSIC, Estación Experimental del ZaidínGranadaSpain
  4. 4.Bio-Illiberis R&DArmillaSpain
  5. 5.Facultad de CienciasUniversidad Autónoma de MadridMadridSpain
  6. 6.CSIC, Centro de Investigaciones BiológicasMadridSpain
  7. 7.CSIC, Centro Nacional de BiotecnologíaMadridSpain
  8. 8.Universidad Pablo de OlavideSevillaSpain
  9. 9.Fundación MedinaArmillaSpain
  10. 10.SecugenMadridSpain

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