Effect of Relative Humidity on Deinococcus radiodurans’ Resistance to Prolonged Desiccation, Heat, Ionizing, Germicidal, and Environmentally Relevant UV Radiation
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To test the effect of humidity on the radiation resistance of Deinococcus radiodurans, air-dried cells were irradiated with germicidal 254 nm UV, and simulated environmental UV or γ-radiation and survival was compared to cells in suspension. It was observed that desiccated cells exhibited higher levels of resistance than cells in suspension toward UV or γ-radiation as well as after 85°C heat shock. It was also shown that low relative humidity improves survival during long-term storage of desiccated D. radiodurans cells. It can be concluded that periods or environments in which cells exist in a dehydrated state are beneficial for D. radiodurans’ survival exposed to various other stresses.
KeywordsDesiccation Resistance Deinococcus Radiodurans Quartz Disc Nucleotide Excision Repair System Bipyrimidine
The authors are grateful to André Parpart for his excellent technical assistance. We express our thanks to the three anonymous reviewers for their valuable comments.
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