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Microbial Ecology

, Volume 61, Issue 3, pp 660–668 | Cite as

Interactions Between Testate Amoebae and Saprotrophic Microfungi in a Scots Pine Litter Microcosm

  • Martin VohníkEmail author
  • Zuzana Burdíková
  • Aleš Vyhnal
  • Ondřej Koukol
Soil Microbiology

Abstract

In all terrestrial ecosystems, testate amoebae (TA) encounter fungi. There are strong indications that both groups engage in multiple interactions, including mycophagy and decomposition of TA shells, processes which might be fundamental in nutrient cycling in certain ecosystems. Here, we present the results of an experiment focusing on interactions between TA and saprotrophic microfungi colonizing Scots pine (Pinus sylvestris L.) litter needles. The needles were collected from a temperate pine forest and cultivated in damp chambers. Over a few weeks, melanized mycelium of Anavirga laxa Sutton started to grow out of some needles; simultaneously, the common forest-soil TA Phryganella acropodia (Hertwig and Lesser) Hopkinson reproduced and spread around the mycelium. We investigated whether a potential relationship between TA and saprotrophic microfungi exists by comparing the composition of TA communities on and around the needles and testing the spatial relationship between the A. laxa mycelium and P. acropodia shells in the experimental microcosm. Additionally, we asked whether P. acropodia utilized the A. laxa mycelium as a nutrient source and screened whether P. acropodia shells were colonized by the microfungi inhabiting the experimental microcosm. Our results indicate that saprotrophic microfungi may affect the composition of TA communities and their mycelium may affect distribution of TA individuals in pine litter. Our observations suggest that P. acropodia did not graze directly on A. laxa mycelium, but rather fed on its exudates or bacteria associated with the exudates. The fungus Pochonia bulbillosa (Gams & Malla) Zare & Gams was often found parasitising encysted shells or decomposing already dead individuals of P. acropodia. TA and pine litter microfungi engage in various direct and indirect interactions which are still poorly understood and deserve further investigation. Their elucidation will improve our knowledge on fundamental processes influencing coexistence of soil microflora and microfauna.

Keywords

Fungal Hypha Experimental Microcosm Total Microbial Biomass Testate Amoeba Needle Surface 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgement

This study was supported by the Academy of Sciences of the Czech Republic (research projects AV0Z60050516 and AV0Z50110509), Ministry of Education, Youth and Sports (research program LC06063) and Grant Agency of the Czech Republic (206/09/P295 and 204/09/H084). The authors thank David M. Wilkinson and Humphrey G. Smith for valuable comments on the results of the experiment, four anonymous reviewers for their valuable effort, and Jesse J. Sadowsky for a stylistic revision. MV thanks the foundation “Nadání Josefa, Marie a Zdenky Hlávkových” for funding his stay at Liverpool John Moores University, Liverpool, UK.

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Martin Vohník
    • 1
    • 2
    Email author
  • Zuzana Burdíková
    • 3
    • 4
  • Aleš Vyhnal
    • 4
  • Ondřej Koukol
    • 5
  1. 1.Department of Mycorrhizal SymbiosesInstitute of Botany ASCRPrůhoniceCzech Republic
  2. 2.Department of Plant Experimental Biology, Faculty of ScienceCharles University in PraguePragueCzech Republic
  3. 3.Department of Paleontology, Faculty of ScienceCharles University in PraguePragueCzech Republic
  4. 4.Department of BiomathematicsInstitute of Physiology ASCRPragueCzech Republic
  5. 5.Department of Botany, Faculty of ScienceCharles University in PraguePragueCzech Republic

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