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Microbial Ecology

, Volume 61, Issue 3, pp 626–634 | Cite as

Interactions Between the Yeast Ogataea pini and Filamentous Fungi Associated with the Western Pine Beetle

  • Thomas S. DavisEmail author
  • Richard W. Hofstetter
  • Jeffrey T. Foster
  • Nathaniel E. Foote
  • Paul Keim
Fungal Microbiology

Abstract

Ecologically important microbes other than filamentous fungi can be housed within the fungal-transport structures (mycangia) of Dendroctonus bark beetles. The yeast Ogataea pini (Saccharomycetales: Saccharomycetaceae) was isolated from the mycangia of western pine beetle (Dendroctonus brevicomis) populations in northern Arizona (USA) with a frequency of 56%. We performed a series of in vitro assays to test whether volatile organic compounds produced by O. pini affected radial growth rates of mutualistic and antagonistic species of filamentous fungi that are commonly found in association with the beetle including Entomocorticium sp. B, Ophiostoma minus, Beauvaria bassiana, and an Aspergillus sp. We determined the compounds O. pini produced when grown on 2% malt extract agar using a gas chromatography/mass spectrometry (GC/MS) analysis of headspace volatiles. Volatiles produced by O. pini on artificial media significantly enhanced the growth of the mutualistic Entomocorticium sp. B, and inhibited growth of the entomopathogenic fungus B. bassiana. GC/MS revealed that O. pini produced ethanol, carbon disulfide (CS2), and Δ-3-carene in headspace. The results of these studies implicate O. pini as an important component in D. brevicomis community ecology, and we introduce multiple hypotheses for future tests of the effects of yeasts in the symbiont assemblages associated with Dendroctonus bark beetles.

Keywords

Volatile Organic Compound Filamentous Fungus Bark Beetle Radial Growth Rate Pinus Ponderosa 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The authors thank the NSF IGERT Program at NAU and the ARCS Foundation, Inc. (Phoenix, AZ) for providing funding that contributed to the completion of this work. Partial funding and work space was provided by the US Forest Service, Rocky Mountain Research Station (05-JV-11221615-104 and 08-JV-11221633-250). We thank Brandy Francis for assistance with DNA extractions and sequencing. We also thank C. Currie, W. Murray, and J. Hulcr for insights that improved the quality of this work, and Marilee Sellers for SEM imaging at the NAU Imaging and Histology Core Facility. The authors thank Impact Analytical for providing the technical expertise and laboratory support needed to perform the GC/MS analysis. Finally, we would like to acknowledge Kyria Boundy-Mills for clarifying yeast taxonomy.

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Thomas S. Davis
    • 1
    Email author
  • Richard W. Hofstetter
    • 1
  • Jeffrey T. Foster
    • 2
  • Nathaniel E. Foote
    • 1
  • Paul Keim
    • 2
  1. 1.School of Forestry, Southwest Forest Science Complex (82)Northern Arizona UniversityFlagstaffUSA
  2. 2.The Microbial Genomics and Genetics CenterNorthern Arizona UniversityFlagstaffUSA

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