Microbial Ecology

, Volume 60, Issue 3, pp 644–654

Mosquito-Bacteria Symbiosis: The Case of Anopheles gambiae and Asaia

  • Claudia Damiani
  • Irene Ricci
  • Elena Crotti
  • Paolo Rossi
  • Aurora Rizzi
  • Patrizia Scuppa
  • Aida Capone
  • Ulisse Ulissi
  • Sara Epis
  • Marco Genchi
  • N’Fale Sagnon
  • Ingrid Faye
  • Angray Kang
  • Bessem Chouaia
  • Cheryl Whitehorn
  • Guelbeogo W. Moussa
  • Mauro Mandrioli
  • Fulvio Esposito
  • Luciano Sacchi
  • Claudio Bandi
  • Daniele Daffonchio
  • Guido Favia
Host Microbe Interactions

DOI: 10.1007/s00248-010-9704-8

Cite this article as:
Damiani, C., Ricci, I., Crotti, E. et al. Microb Ecol (2010) 60: 644. doi:10.1007/s00248-010-9704-8

Abstract

The symbiotic relationship between Asaia, an α-proteobacterium belonging to the family Acetobacteriaceae, and mosquitoes has been studied mainly in the Asian malaria vector Anopheles stephensi. Thus, we have investigated the nature of the association between Asaia and the major Afro-tropical malaria vector Anopheles gambiae. We have isolated Asaia from different wild and laboratory reared colonies of A. gambiae, and it was detected by PCR in all the developmental stages of the mosquito and in all the specimens analyzed. Additionally, we have shown that it localizes in the midgut, salivary glands and reproductive organs. Using recombinant strains of Asaia expressing fluorescent proteins, we have demonstrated the ability of the bacterium to colonize A. gambiae mosquitoes with a pattern similar to that described for A. stephensi. Finally, fluorescent in situ hybridization on the reproductive tract of females of A. gambiae showed a concentration of Asaia at the very periphery of the eggs, suggesting that transmission of Asaia from mother to offspring is likely mediated by a mechanism of egg-smearing. We suggest that Asaia has potential for use in the paratransgenic control of malaria transmitted by A. gambiae.

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Claudia Damiani
    • 1
  • Irene Ricci
    • 1
  • Elena Crotti
    • 2
  • Paolo Rossi
    • 1
  • Aurora Rizzi
    • 2
  • Patrizia Scuppa
    • 1
  • Aida Capone
    • 1
  • Ulisse Ulissi
    • 1
  • Sara Epis
    • 1
  • Marco Genchi
    • 3
  • N’Fale Sagnon
    • 4
  • Ingrid Faye
    • 5
  • Angray Kang
    • 6
  • Bessem Chouaia
    • 2
  • Cheryl Whitehorn
    • 7
  • Guelbeogo W. Moussa
    • 4
  • Mauro Mandrioli
    • 8
  • Fulvio Esposito
    • 1
  • Luciano Sacchi
    • 3
  • Claudio Bandi
    • 9
  • Daniele Daffonchio
    • 2
  • Guido Favia
    • 1
  1. 1.Scuola di Bioscienze e BiotecnologieUniversità degli Studi di CamerinoCamerinoItaly
  2. 2.Dipartimento di Scienze e Tecnologie Alimentari e MicrobiologicheUniversità degli Studi di MilanoMilanItaly
  3. 3.Dipartimento di Biologia AnimaleUniversità degli Studi di PaviaPaviaItaly
  4. 4.Centre National de Recherche et de Formation sur le Paludisme (CNRFP)Ouagadougou 01Burkina Faso
  5. 5.Department of Genetics Microbiology and ToxicologyStockholm UniversityStockholmSweden
  6. 6.Department of Molecular and Applied BiosciencesUniversity of WestminsterLondonUK
  7. 7.Department of Infectious and Tropical DiseasesLondon School of Hygiene and Tropical MedicineLondonUK
  8. 8.Dipartimento di BiologiaUniversità degli Studi di Modena e Reggio EmiliaModenaItaly
  9. 9.Dipartimento di Patologia Animale, Igiene e Sanità Pubblica VeterinariaUniversità degli Studi di MilanoMilanItaly

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