Microbial Ecology

, Volume 60, Issue 4, pp 740–752 | Cite as

Bacterial Diversity of Weathered Terrestrial Icelandic Volcanic Glasses

  • Laura C. Kelly
  • Charles S. Cockell
  • Yvette M. Piceno
  • Gary L. Andersen
  • Thorsteinn Thorsteinsson
  • Viggo Marteinsson
Environmental Microbiology

Abstract

The diversity of microbial communities inhabiting two terrestrial volcanic glasses of contrasting mineralogy and age was characterised. Basaltic glass from a <0.8 Ma hyaloclastite deposit (Valafell) harboured a more diverse Bacteria community than the younger rhyolitic glass from ∼150-300 AD (Dόmadalshraun lava flow). Actinobacteria dominated 16S rRNA gene clone libraries from both sites, however, Proteobacteria, Acidobacteria and Cyanobacteria were also numerically abundant in each. A significant proportion (15-34%) of the sequenced clones displayed <85% sequence similarities with current database sequences, thus suggesting the presence of novel microbial diversity in each volcanic glass. The majority of clone sequences shared the greatest similarity to uncultured organisms, mainly from soil environments, among these clones from Antarctic environments and Hawaiian and Andean volcanic deposits. Additionally, a large number of clones within the Cyanobacteria and Proteobacteria were more similar to sequences from other lithic environments, included among these Icelandic clones from crystalline basalt and rhyolite, however, no similarities to sequences reported from marine volcanic glasses were observed. PhyloChip analysis detected substantially greater numbers of phylotypes at both sites than the corresponding clone libraries, but nonetheless also identified the basaltic glass community as the richer, containing approximately 29% unique phylotypes compared to rhyolitic glass.

Supplementary material

248_2010_9684_Fig6_ESM.gif (78 kb)
Supplemental Information Figure 1

ANOSIM analyis of DGGE profiles. Pal basaltic glass/palagonite samples from Valafell, Obs rhyolitic glass/obsidian samples from Dόmadalshraun. Samples ending in C are composites for the given glass type (GIF 77 kb)

248_2010_9684_MOESM1_ESM.tif (661 kb)
High-resolution image (TIFF 661 kb)

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Laura C. Kelly
    • 1
  • Charles S. Cockell
    • 1
  • Yvette M. Piceno
    • 2
  • Gary L. Andersen
    • 2
  • Thorsteinn Thorsteinsson
    • 3
  • Viggo Marteinsson
    • 4
  1. 1.Geomicrobiology Research Group, Planetary and Space Sciences Research InstituteOpen UniversityMilton KeynesUK
  2. 2.Ecology Department, Earth Sciences DivisionLawrence Berkeley National LaboratoryBerkeleyUSA
  3. 3.Hydrology DivisionNational Energy AuthorityReykjavikIceland
  4. 4.Matís ohf./Icelandic Food and Biotech R&DReykjavikIceland

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