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Microbial Ecology

, Volume 61, Issue 1, pp 20–30 | Cite as

Changes in the Intestinal Microbiota of Wild Atlantic cod Gadus morhua L. Upon Captive Rearing

  • Anusha K. S. Dhanasiri
  • Laila Brunvold
  • Monica F. Brinchmann
  • Kjetil Korsnes
  • Øivind Bergh
  • Viswanath KironEmail author
Host Microbe Interactions

Abstract

The commensal microbiota plays an important role in the well-being of the host organism, and it would be worthwhile to know the tenacious communities among them. Therefore, a study was undertaken to examine the changes in constitution of the intestinal microbiota of wild fish consequential to captivity. At first, the composition of intestinal microorganisms of Atlantic cod caught from the coastal area off Bodø, Norway, was examined. Thereafter, the changes in the bacterial community of the captive fish after offering them artificial feed or subjecting them to starvation were studied. The microbiota from the intestinal contents and wall segments were analyzed quantitatively by spread plate technique and DAPI staining and qualitatively by denaturing gradient gel electrophoresis. The study revealed that the counts of intestinal microbes in wild-caught Atlantic cod were not affected by captive rearing for 6 weeks, either when fed or when starved. However, the diversity of intestinal bacterial community was reduced in response to artificial feeding, whereas the change was restricted upon starvation.

Keywords

Artificial Diet Intestinal Microbiota Posterior Intestine Culturable Count Intestinal Bacterial Community 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This research is part of the master’s thesis of the first author, funded in parts by Bodø University College and by Research Council of Norway (Project176528/V10). The authors would like to thank Morten Krogstad, Engineer, Mørkvedbukta Research Station, for procuring the experimental animals.

Supplementary material

248_2010_9673_MOESM1_ESM.ppt (200 kb)
Fig. S1 The lanes and bands identified in the Gel W using Quantity One® program. The gel has DGGE profiles generated from intestinal bacteria of wild Atlantic cod obtained from posterior wall samples. Lanes 1 and 17: markers (M); lanes 2–6: fish soon after capture (I1–I5); lanes 7–11: kept unfed in captivity (U1–U5); lanes 12–16: reared in captivity with artificial feed (F1–F5). (PPT 200 kb)

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Anusha K. S. Dhanasiri
    • 1
  • Laila Brunvold
    • 2
  • Monica F. Brinchmann
    • 1
  • Kjetil Korsnes
    • 1
  • Øivind Bergh
    • 2
  • Viswanath Kiron
    • 1
    Email author
  1. 1.Faculty of Biosciences and AquacultureBodø University CollegeBodøNorway
  2. 2.Institute of Marine ResearchBergenNorway

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