In Vitro and In Vivo Evaluation of the Prebiotics GroBiotic®-A, Inulin, Mannanoligosaccharide, and Galactooligosaccharide on the Digestive Microbiota and Performance of Hybrid Striped Bass (Morone chrysops × Morone saxatilis)
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Two separate experiments were conducted with hybrid striped bass to evaluate four potential prebiotics: GroBiotic®-A (partially autolyzed brewer’s yeast, dairy ingredient components, and fermentation products), mannanoligosaccharide (MOS), galactooligosaccharide (GOS), and inulin. In the in vitro experiment, intestinal contents were incubated with the individual prebiotics (0.5% by weight) at 25°C for 24 and 48 h. Analysis of volatile fatty acids in the supernatant showed that GroBiotic®-A, MOS, and GOS tended to produce lower acetate levels but higher butyrate levels at 48 h compared to diet alone. However, denaturing gradient gel electrophoresis (DGGE) analysis failed to detect any differences in the composition of the microbial community among treatments. DNA sequencing of a common band for all inoculated samples revealed close similarity to the anaerobic Fusobacteria bacterium. An 8-week feeding trial also was conducted to evaluate the four prebiotics looking at growth performance; weight gain, feed efficiency ratio, protein efficiency ratio, whole-body ash, moisture, and lipid did not vary among fish fed the various diets. However, DGGE analysis revealed that all prebiotics produced a different type of microbial community in the intestinal tract of hybrid striped bass compared to fish fed the basal diet. Thus, GroBiotic®-A, FOS, GOS, and MOS exhibited prebiotic effects in hybrid striped bass.
KeywordsMicrobial Community Inulin Basal Diet Arctic Charr Protein Efficiency Ratio
The authors would like to thank Texas Sea Grant College Program for funding this research. We also would like to thank International Ingredient Corporation (St. Louis, MO, USA) for providing the GroBiotic®-A, Alltech Biotechnology for the Bio-MOS®, Friesland Foods, Inc. for the Vivinal GOS, and Encore Technologies for the inulin. Keo Fish Farms generously provided the hybrid striped bass used in this study.
- 2.Bakke-McKellep AM, Penn MH, Salas PM, Refstie S, Sperstad S, Landsverk T, Krogdahl Å (2007) Effects of dietary soybean meal, inulin and oxytetracycline on intestinal microbiota and epithelial cell stress, apoptosis and proliferation in the teleost Atlantic Salmon (Salmo salar L.). Br J Nutr 97:699CrossRefPubMedGoogle Scholar
- 5.Burr GS, Li P, Goff JB, Grisdale-Helland B, Hellend SJ, Gatlin DM III (2006) Evaluation of growth performance and whole-body composition of juvenile hybrid striped bass Morone chrysops x Morone saxatilis and red drum Sciaenops ocellatus fed high-protein and high-lipid diets. J World Aquacult Soc 37:421CrossRefGoogle Scholar
- 6.Burr G, Hume M, Gatlin DM III (2007) Effects of prebiotics on nutrient digestibility of a soybean meal-based diet by red drum Sciaenops ocellatus (Linnaeus). Aquac Res 39:1680Google Scholar
- 7.De De Hertogh G, Aerssens J, De Hoogt R, Peeters P, Verhasselt P, Van Eyken P, Ectors N, Vermeire S, Rutgeerts P, Coulie B, Geboes K (2006) Validation of 16S rDNA sequencing in microdissected bowel biopsies from Crohn’s disease patients to assess bacterial flora diversity. J Pathol 209:532CrossRefPubMedGoogle Scholar
- 10.Heikkinen J, Vielma J, Kemiläinen O, Tiirola M, Eskelinen P, Kiuru T, Navia Paldanius E, von Wright A (2006) Effects of soybean meal based diet on growth performance, gut histopathology and intestinal microbiotia of juvenile rainbow trout (Oncorhynchus mykiss). Aquaculture 261:259CrossRefGoogle Scholar
- 12.Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST (eds) (1994) Bergey’s manual of determinative bacteriology. Group 6, Gram-negative, anaerobic, straight, curved, and helical bacteria, vol 9. Williams & Wilkins, Baltimore, p 291Google Scholar
- 15.Johnson EA (1990) Foodborne Diseases. Clostridium perfringens food poisoning. Academic, California, p 229Google Scholar
- 35.SAS Institute Inc (1985) SAS User’s guide statistics, Version 5.2 ed. SAS Institute Inc, Cary, p 956Google Scholar