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Microbial Ecology

, Volume 55, Issue 4, pp 723–736 | Cite as

Functional Characterization of the Microbial Community in Geothermally Heated Marine Sediments

  • Antje Rusch
  • Jan P. Amend
Original Article

Abstract

The microbial population of geothermally heated sediments in a shallow bay of Vulcano Island (Italy) was characterized with respect to metabolic activities and the putatively catalyzing hyperthermophiles. Site-specific anoxic culturing media, most of which were amended with combinations of electron donors (glucose or carboxylic acids) and acceptors (sulfate), were used for selective enrichment of metabolically defined subpopulations. The mostly archaeal chemoautotrophs produced formate at rates of 3.25 and 0.46 fmol cell−1 day−1 with and without sulfate, respectively. The glucose fermenting heterotrophs produced acetate (18 fmol cell−1 day−1) and lactate (2.6 fmol cell−1 day−1) and were identified as predominantly Thermus sp. and coccoid archaea. These archaeal cells also metabolized lactate (5.6 fmol cell−1 day−1), but neither formate nor acetate. The heterotrophic culture enriched on formate/acetate/propionate/sulfate utilized mainly formate (27 fmol cell−1 day−1) and lactate (89–195 fmol cell−1 day−1), and consumed sulfate (38–68 fmol cell−1 day−1). These formate or lactate consuming sulfate reducers were dominated by Archaeoglobales (7% in situ) and unidentified Archaea. The in situ benthic community comprised 15% Crenarchaeota, a significant group only in the autotrophic cultures, and 3% Thermus sp., the putatively predominant group involved in fermentative metabolism. The role of Thermoccales (4% in situ) remained undisclosed in our experiments. This first comprehensive data set established plausible links between several groups of hyperthermophiles in shallow marine hydrothermal systems, their metabolic function within the benthic microbial community, and biogeochemical turnover rates.

Keywords

Archaea Sulfate Reducer Enrichment Culture Incubation Experiment Heterotrophic Culture 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The authors gratefully appreciate the support of Sergio Gurrieri (INGV Palermo) during our excursion to Vulcano Island. We thank Andrea Amend for preparing growth media and for the chromatographic analyses. This research was funded by NSF grant EAR-0447231.

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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  1. 1.Department of Earth and Planetary SciencesWashington UniversitySt. LouisUSA
  2. 2.Department of Geology and GeophysicsUniversity of Hawaii at ManoaHonoluluUSA

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