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Microbial Ecology

, Volume 55, Issue 1, pp 152–161 | Cite as

Changes in Morphology and Elemental Composition of Vibrio splendidus Along a Gradient from Carbon-limited to Phosphate-limited Growth

  • Trond Løvdal
  • Evy F. Skjoldal
  • Mikal Heldal
  • Svein Norland
  • T. Frede Thingstad
Article

Abstract

We examined morphology, elemental composition (C, N, P), and orthophosphate-uptake efficiency in the marine heterotrophic bacterium Vibrio splendidus grown in continuous cultures. Eight chemostats were arranged along a gradient of increasing glucose concentrations in the reservoirs, shifting the limiting factor from glucose to phosphate. The content of carbon, nitrogen, and phosphorus was measured in individual cells by x-ray microanalysis using a transmission electron microscope (TEM). Cell volumes (V) were estimated from length and width measurements of unfixed, air-dried cells in TEM. There was a transition from coccoid cells in C-limited cultures toward rod-shaped cells in P-limited cultures. Cells in P-limited cultures with free glucose in the media were significantly larger than cells in glucose-depleted cultures (P < 0.0001). We found functional allometry between cellular C-, N-, and P content (in femtograms) and V (in cubic micrometers) in V. splendidus (C = 224 × V 0.89, N = 52.5 × V 0.80, P = 2 × V 0.65); i.e., larger bacteria had less elemental C, N, and P per V than smaller cells, and also less P relative to C. Biomass-specific affinity for orthophosphate uptake in large P-limited V. splendidus approached theoretical maxima predicted for uptake limited by molecular diffusion toward the cells. Comparing these theoretical values to respective values for the smaller, coccoid, C-limited V. splendidus indicated, contrary to the traditional view, that large size did not represent a trade-off when competing for the non-C-limiting nutrients.

Keywords

Vibrio Soluble Reactive Phosphorus Free Glucose Cell Quota Late Logarithmic Phase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work was supported by the Strategic Institution Programme “Patterns in Biodiversity” Contract 158936/S40 from the Research Council of Norway. The FACSCalibur flow cytometer was in part funded by a grant from The Knut and Alice Wallenberg Foundation to the Virtue program. We gratefully thank Philippe Lebaron for providing the V. splendidus culture, Tsuneo Tanaka for advice and assistance, George Jackson for providing information on diffusion literature, and Egil S. Erichsen for his assistance at the Laboratory for Electron Microscopy (LEM), Science Faculty, University of Bergen.

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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Trond Løvdal
    • 1
  • Evy F. Skjoldal
    • 1
  • Mikal Heldal
    • 1
  • Svein Norland
    • 1
  • T. Frede Thingstad
    • 1
  1. 1.Department of BiologyUniversity of BergenBergenNorway

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