Bacterial Diversity of East Calcutta Wet Land Area: Possible Identification of Potential Bacterial Population for Different Biotechnological Uses
- 368 Downloads
The extent of microbial diversity in nature is still largely unknown, suggesting that there might be many more useful products yet to be identified from soil microorganisms. This insight provides the scientific foundation for a renewed interest in examining soil microorganisms for novel commercially important products. This has led us to access the metabolic potential of soil microorganisms via cultivation strategy. Keeping this in mind, we have performed a culture-dependent survey of important soil bacterial community diversity in East Calcutta Wetland area (Dhapa Landfill Area). We describe isolation of 38 strains, their phenotypic and biochemical characterization, and finally molecular identification by direct sequencing of polymerase chain reaction (PCR)-amplified 16S rRNA gene products. We have isolated and identified strains able to fix nitrogen, produce extracellular enzymes like protease, cellulase, xylanase, and amylase, and solubilize inorganic phosphates. Some isolates can synthesize extracellular insecticidal toxins. We find a good correlation between biochemical and phenotypic behavior and the molecular study using 16S rRNA gene of the isolates. Furthermore, our findings clearly indicate the composition of cultivable soil bacteria in East Calcutta Wetland Area.
KeywordsCellulase Pectin Feather Meal Rhodococcus Strain Insecticidal Toxin
Abhrajyoti Ghosh thanks the Guha Research Endowment Fund for financial assistance. This work was partially financed by Sun Biotechnology Ltd., Kolkata, India. The authors also acknowledged the support from Mr. Aloke Sen and Dr. Kalyan Chakraborty.
- 17.Head, IM, Saunders, JR, Pickup, RW (1998) Microbial evolution, diversity and ecology: a decade of ribosomal RNA analysis of uncultivated microorganisms. Microb Ecol 23: 45–54Google Scholar
- 19.Holtz, JD (1993) Bergey’s Manual of Determinative Bacteriology, 9th edn. Williams and Wilkins, BaltimoreGoogle Scholar
- 20.Lane, DJ (1991) 16S/23S rRNA sequencing. In: Stackebrandt E, Goodfellow M (Eds.) Nucleic Acid Techniques in Bacterial Systematics. John Wiley & Sons, New York NY, pp 115–175Google Scholar
- 21.Leisack, W, Jessen, PH, Rainey, FA, Ward-Rainey, N, Stackebrandt, E (1997) Microbial diversity in soil: the need for a combined approach using molecular and cultivation techniques. In: vanElsas, JD, Trevors JT, Wellington, EMH (Eds.) Modern Soil Microbiology. Marcel Dekker Inc., New York NY, pp 375–439Google Scholar
- 26.Olaniya, MS, Sur, MS, Bhide, AD, Swarnakar, SN (1998) Heavy metals pollution of agricultural soil and vegetation due to application of solid waste—a case study. Indian J Environ Health 40(2): 160–168Google Scholar
- 28.Perucci, P (1990) Effect of the addition of municipal soil waste compost on microbiological biomass and enzyme activities in soil. Biol Fertil Soils 10: 221–226Google Scholar
- 30.Pikovskaya, RI (1948) Mobilisation of phosphorous in soil in connection with the vital activity of some microbial species. Microbiologia 17: 362–370Google Scholar
- 31.Sambrook, J, Russel, DW (2001) Molecular cloning: A laboratory manual. 3rd edn. CSH Laboratory Press, Cold Spring Harbor, New YorkGoogle Scholar