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Microbial Ecology

, Volume 53, Issue 3, pp 471–474 | Cite as

Pseudomonas fluorescens SBW25 Biofilm and Planktonic Cells Have Differentiable Raman Spectral Profiles

  • Wei E. Huang
  • Susanne Ude
  • Andrew J. Spiers
Article

Abstract

Biofilms, and other bacterial aggregations, are of significance in both environmental microbiology and in plant and human pathogenesis. Comparative single-cell Raman spectral analysis can differentiate between planktonic bacteria and those recovered from biofilms and appears to offer a new means by which to investigate bacterial cell physiology, metabolic status, and stress under different environmental conditions.

Keywords

Pseudomonas Fluorescens Planktonic Cell Planktonic Sample Planktonic Bacterium Wellcome Trust Sanger Institute 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

We are grateful to Andrew Knowles and Simon FitzGerald from HORIBA Jobin-Yvon Ltd. UK for supplying the Raman Confocal Microscope.

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Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Wei E. Huang
    • 1
    • 2
  • Susanne Ude
    • 1
    • 2
  • Andrew J. Spiers
    • 1
    • 2
  1. 1.Molecular Microbiology Ecology and Environmental Biotechnology SectionsCEH-OxfordOxfordUK
  2. 2.Department of Plant SciencesUniversity of OxfordOxfordUK

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