Microbial Ecology

, Volume 53, Issue 3, pp 399–413 | Cite as

Community Structure of Actively Growing Bacterial Populations in Plant Pathogen Suppressive Soil

  • Karin Hjort
  • Antje Lembke
  • Arjen Speksnijder
  • Kornelia Smalla
  • Janet K. Jansson
Article

Abstract

The bacterial community in soil was screened by using various molecular approaches for bacterial populations that were activated upon addition of different supplements. Plasmodiophora brassicae spores, chitin, sodium acetate, and cabbage plants were added to activate specific bacterial populations as an aid in screening for novel antagonists to plant pathogens. DNA from growing bacteria was specifically extracted from the soil by bromodeoxyuridine immunocapture. The captured DNA was fingerprinted by terminal restriction fragment length polymorphism (T-RFLP). The composition of the dominant bacterial community was also analyzed directly by T-RFLP and by denaturing gradient gel electrophoresis (DGGE). After chitin addition to the soil, some bacterial populations increased dramatically and became dominant both in the total and in the actively growing community. Some of the emerging bands on DGGE gels from chitin-amended soil were sequenced and found to be similar to known chitin-degrading genera such as Oerskovia, Kitasatospora, and Streptomyces species. Some of these sequences could be matched to specific terminal restriction fragments on the T-RFLP output. After addition of Plasmodiophora spores, an increase in specific Pseudomonads could be observed with Pseudomonas-specific primers for DGGE. These results demonstrate the utility of microbiomics, or a combination of molecular approaches, for investigating the composition of complex microbial communities in soil.

Notes

Acknowledgments

This work was funded by the EU Project, METACONTROL (QLK 3-2002-2068). Plasmodiophora brassicae spores were kindly provided by Hanna Friberg, Department of Ecology and Crop Production Science, Swedish University of Agricultural Sciences, Uppsala, Sweden.

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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Karin Hjort
    • 1
  • Antje Lembke
    • 2
  • Arjen Speksnijder
    • 3
  • Kornelia Smalla
    • 2
  • Janet K. Jansson
    • 1
  1. 1.Department of MicrobiologySwedish University of Agricultural SciencesUppsalaSweden
  2. 2.Federal Biological Research Centre for Agriculture and Forestry (BBA)BraunschweigGermany
  3. 3.Plant Research International B.V.WageningenThe Netherlands

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