Microbial Ecology

, Volume 53, Issue 4, pp 579–590 | Cite as

A Phylogenetic Evaluation of Whether Endophytes Become Saprotrophs at Host Senescence

  • Itthayakorn Promputtha
  • Saisamorn Lumyong
  • Vijaykrishna Dhanasekaran
  • Eric Huge Charles McKenzie
  • Kevin David Hyde
  • Rajesh Jeewon
Article
First Online:
Received:
Revised:
Accepted:

Abstract

Fungal endophytes and saprotrophs generally play an important ecological role within plant tissues and dead plant material. Several reports based solely on morphological observations have postulated that there is an intimate link between endophytes and saprotrophs. This study aims to provide valuable insight as to whether some endophytic fungi manifest themselves as saprotrophs upon host decay. Ribosomal DNA-based sequence comparison and phylogenetic relationships from 99 fungal isolates (endophytes, mycelia sterilia, and saprotrophs) recovered from leaves and twigs of Magnolia liliifera were investigated in this study. Molecular data suggest there are fungal taxa that possibly exist as endophytes and saprotrophs. Isolates of Colletotrichum, Fusarium, Guignardia, and Phomopsis, which are common plant endophytes, have high sequence similarity and are phylogenetically related to their saprotrophic counterparts. This provides evidence to suggest that some endophytic species change their ecological strategies and adopt a saprotrophic lifestyle. The implication of these findings on fungal biodiversity and host specificity is also discussed.

Keywords

Fusarium Terminal Restriction Fragment Length Polymorphism Fusarium Species Fungal Endophyte Markov Chain Monte Carlo Method 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Notes

Acknowledgments

We thank the Hong Kong Research Grants Council and the University of Hong Kong for providing funds (HKU 7322/04M; CRCG 10205773) to Dr. R. Jeewon and Dr K.D. Hyde to carry out the molecular work and manuscript preparation, and for providing support to I. Promputtha with a training studentship and D. Vijaykrishna with a studentship. Funds for this research were also provided by the Royal Golden Jubilee Ph.D. Program (4.B.CM/44/D.1) under the Thailand Research Fund. S. Lumyong thanks JSPS-NRCT (2003–2005) for support. Shenoy B.D., Helen Leung, and Heidi Kong are thanked for assistance.

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Copyright information

© Springer Science+Business Media, Inc. 2007

Authors and Affiliations

  • Itthayakorn Promputtha
    • 1
  • Saisamorn Lumyong
    • 1
  • Vijaykrishna Dhanasekaran
    • 2
  • Eric Huge Charles McKenzie
    • 3
  • Kevin David Hyde
    • 2
  • Rajesh Jeewon
    • 2
  1. 1.Department of Biology, Faculty of ScienceChiang Mai UniversityChiang MaiThailand
  2. 2.Centre for Research in Fungal Diversity, Department of Ecology and BiodiversityUniversity of Hong KongHong KongChina
  3. 3.Landcare ResearchAucklandNew Zealand

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