Culturable Leaf-Associated Bacteria on Tomato Plants and Their Potential as Biological Control Agents
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Culturable leaf-associated bacteria inhabiting a plant have been considered as promising biological control agent (BCA) candidates because they can survive on the plant. We investigated the relationship between bacterial groups of culturable leaf-associated bacteria on greenhouse- and field-grown tomato leaves and their antifungal activities against tomato diseases in vitro and in vivo. In addition, the isolated bacteria were analyzed for N-acyl-homoserine lactone (AHL) and indole-3-acetic acid (IAA) production, which have been reported to associate with bacterial colonization, and resistance to a tomato alkaloid (α-tomatine). Leaf washings and subsequent leaf macerates were used to estimate the population size of epiphytic and more internal bacteria. Bacterial population sizes on leaves at the same position increased as the leaves aged under both greenhouse and field conditions. Field-grown tomatoes had significantly larger population sizes than greenhouse-grown tomatoes. Analysis of 16S rRNA gene (rDNA) sequencing using 887 culturable leaf-associated bacteria revealed a predominance of the Bacillus and Pseudomonas culturable leaf-associated bacterial groups on greenhouse- and field-grown tomatoes, respectively. Curtobacterium and Sphingomonas were frequently recovered from both locations. From the 2138 bacterial strains tested, we selected several strains having in vitro antifungal activity against three fungal pathogens of tomato: Botrytis cinerea, Fulvia fulva, and Alternaria solani. Among bacterial strains with strong in vitro antifungal activities, Bacillus and Pantoea tended to show strong antifungal activities, whereas Curtobacterium and Sphingomonas were not effective. The results indicated the differences in antifungal activity among predominant bacterial groups. Analysis of α-tomatine resistance revealed that most bacterial strains in the dominant groups exhibited moderate or high resistance to α-tomatine in growth medium. Furthermore, some Sphingomonas and Pantoea strains showed AHL and IAA production activities. Strain 125NP12 (Pantoea ananatis) showed particular α-tomatine resistance, and AHL and IAA production had the highest protective value (91.7) against gray mold. Thus, the differences of these physiological properties among dominant bacteria may be associated with the disease suppression ability of BCAs on tomato plants.
KeywordsSphingomonas Gray Mold Tomato Leave Pantoea Strong Antifungal Activity
We are grateful to Dr. Naoyuki Matsumoto (National Institute for Agro-Environmental Sciences) and Dr. Takako Sakai (Kyowa Seed Co., Ltd.) for suggestions and critical reading of the manuscript. The authors thank Dr. Linda L. Kinkel (University of Minnesota) for help with the AHLs detection. Thanks are due to Rie Adachi, Yuriko Watanabe, Nobutaka Numaziri, and Machiko Imai for technical assistance.
- 2.Arwiyanto, T, Sakata, K, Goto, M, Thuyumu, S, Takikawa, Y (1994) Induction of tomatine in tomato plant by an avirulent strain of Pseudomonas solanacearum. Ann Phytopathol Soc Jpn 60: 288–294Google Scholar
- 5.Beimen, A, Bermpohl, A, Meletzus, D, Eichenlaub, R, Barz, W (1992) Accumulation of phenolic compounds in leaves of tomato plants after infection with Clavibacter michigamense subsp. michiganense strains differing in virulence. Z Naturforsch [C] 47: 898–909Google Scholar
- 29.Legard, DE, McQuiken, MP, Whipps, JM, Fenlon, JS, Fermor, TR, Thompson, IP, Bailey, MJ, Lynch, JM (1994) Studies of seasonal changes in the microbial populations on the phyllosphere of spring wheat as a prelude to the release of a genetically modified microorganism. Agric Ecosyst Environ 50: 87–101CrossRefGoogle Scholar
- 34.Nishiyama, K, Ezuka, A (1977) Rough-colony isolates of Pseudomonas coronafaciens var. atropurpurea obtained from diseased leaves of ryegrasses. Ann Phytopathol Soc Jpn 43: 426–431 (in Japanese with English summary)Google Scholar
- 40.Thompson, IP, Bailey, MJ, Fenlon, JS, Fermor, TR, Lilley, AK, Lynch, JM, McCormack, PJ, McQuilken, MP, Purdy, KJ, Rainey, PB, Whipps, JM (1993) Quantitative and qualitative seasonal changes in the microbial community from the phyllosphere of sugar beet (Beta vulgaris). Plant Soil 150: 177–191CrossRefGoogle Scholar