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Microbial Ecology

, Volume 52, Issue 1, pp 114–126 | Cite as

Relationships between Stand Composition and Ectomycorrhizal Community Structure in Boreal Mixed-Wood Forests

  • T. DeBellis
  • G. Kernaghan
  • R. Bradley
  • P. WiddenEmail author
Article

Abstract

We investigated the community structure of ectomycorrhizal fungi under varying overstory tree compositions in the southern mixed-wood boreal forest of Quebec. Sampling took place at two locations of differing postfire ages and nine 100-m2 plots were sampled per location. The dominant overstory tree species in the plots were trembling aspen (Populus tremuloides Michx.), white birch (Betula papyrifera Marsh.) or white spruce [Picea glauca (Moench) Voss], and balsam fir [Abies balsamea (L.) Mill.]. Mycorrhizae were analyzed using morphological as well as molecular methods, employing fungal-specific primers to amplify ribosomal DNA for subsequent cloning and sequencing. A total of 1800 mycorrhizal root tips collected from the 18 plots were morphologically classified into 26 morphotypes, with Cenococcum geophilum dominating (36% of root tips). A second set of root tips, selected from the same 18 samples on which the morphological analysis was based, were analyzed using molecular methods. From this analysis, 576 cloned polymerase chain reaction products were screened by restriction fragment length polymorphism analysis and a total of 207 unique types were found. No one type dominated the system and 159 occurred only once. Sequence analysis of the types that occurred more than once revealed that Piloderma sp., Russula sp., Cortinarius sp., and Lactarius sp. were the most common mycorrhizae. The ectomycorrhizal fungal community structure revealed by the rDNA analysis differed from that observed using morphological methods. Canonical correspondence analyses of the sequenced restriction types and % overstory composition indicate that the distributions of ectomycorrhizal fungi are influenced by the relative proportions of host tree species. The distinct fungal assemblages found in the different plots supported by the different combinations of host tree species provides further support for the need to conserve stand diversity in the southern boreal forest.

Keywords

Canonical Correspondence Analysis Overstory Tree Lactarius Host Tree Species RFLP Type 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This study was supported by a grant from the Natural Sciences and Engineering Research Council of Canada (NSERC) to P.W. and a strategic grant by NSERC to R.B. (P.I.), Y. Bergeron, and P.W. Further support of this work came from Le Fonds québécois de la recherche sur la nature et les technologies, J.W. McConnell, Groupe de recherche en écologie forestière interuniversitaire, Power Corporation, and the Canadian–Italian Business Professional Association fellowships to T.D. We greatly thank Goldie Marmor for her laboratory assistance, Sarah McNair for her field assistance, Sonia Legaré for her soil chemistry data, and Dr. S. Dayanandan for his valuable comments on earlier versions of this manuscript.

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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • T. DeBellis
    • 1
  • G. Kernaghan
    • 2
  • R. Bradley
    • 3
  • P. Widden
    • 1
    Email author
  1. 1.Department of Biology, Groupe de recherche en écologie forestière interuniversitaire (GREFi)Concordia UniversityMontrealCanada
  2. 2.Département de biologieUniversité LavalSainte-FoyCanada
  3. 3.Département de biologie, Centre de recherche en biologie forestière (CRBF)Université de SherbrookeSherbrookeCanada

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