Genetic and Physiological Characterization of the Intestinal Bacterial Microbiota of Bluegill (Lepomis macrochirus) with Three Different Feeding Habits
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Bluegill (Lepomis macrochirus) in Lake Biwa, Japan, feed on benthic invertebrates (benthivorous type), aquatic plants (herbivorous type), and zooplankton (planktivorous type). To evaluate the effect of food on intestinal bacterial microbiota, we characterized and compared the intestinal microbiota of these three types of bluegill in terms of community-level physiological profile (CLPP) and genetic structure. The CLPP was analyzed using Biolog MicroPlates (Biolog, Inc., Hayward, CA, USA), and multivariate analysis of variance revealed that the CLPP of intestinal microbiota differed significantly between any pairs of the three types of bluegill. The genetic profiles were analyzed by temperature gradient gel electrophoresis of polymerase chain reaction (PCR)-amplified 16S rDNA fragments, and multidimensional scaling indicated the existence of specific intestinal bacterial structures for both the benthivorous and the planktivorous types. These results suggest that the host's feeding habit can be one factor controlling the intestinal microbiota of fish in the natural environment.
KeywordsBacterial Community Intestinal Microbiota Benthic Invertebrate Intestinal Bacterium Terrestrial Insect
We greatly acknowledge S. Gritters and K. Aulwes, Iowa Department of Natural Resources, for fish sampling in Iowa. We thank N. Yamaguchi, C. Sakamoto, F. Maruyama, and other members of Osaka University for their help in the TGGE experiments. We also thank M. Yuma for reviewing an earlier draft of this manuscript, and M. Honjo and M. Ueki for helpful comments and technical assistance. This research was partly supported by the Grant for the Biodiversity Research of the 21st Century COE (A14). The study was also partly supported by the JSPS Grant-in Aid for Basic Research (A) (16207001).
- 1.Bochner, BR (1989) Breathprints at the microbial level. ASM News 55: 536–539Google Scholar
- 3.Campbell, AC, Buswell, JA (1983) The intestinal microflora of farmed Dover sole (Solea solea) at different stages of fish development. J Appl Bacteriol 55: 215–223Google Scholar
- 8.Harmsen, HJM, Wildeboer-Veloo, ACM, Raangs, GC, Wagendorp, AA, Klijn, N, Bindels, JG, Welling, GW (2000) Analysis of intestinal flora development in breast-fed and formula-fed infants by using molecular identification and detection methods. J Pediatr Gastroenterol Nutr 30: 61–67CrossRefPubMedGoogle Scholar
- 20.Schmidt-Nielsen, K (1990) Animal Physiology: Adaptation and Environment, 4th ed. Cambridge University Press, New York, p 319Google Scholar