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Microbial Ecology

, Volume 51, Issue 3, pp 277–284 | Cite as

Genetic and Physiological Characterization of the Intestinal Bacterial Microbiota of Bluegill (Lepomis macrochirus) with Three Different Feeding Habits

  • Kimiko UchiiEmail author
  • Kazuaki Matsui
  • Ryuji Yonekura
  • Katsuji Tani
  • Takehiko Kenzaka
  • Masao Nasu
  • Zen'ichiro Kawabata
Article

Abstract

Bluegill (Lepomis macrochirus) in Lake Biwa, Japan, feed on benthic invertebrates (benthivorous type), aquatic plants (herbivorous type), and zooplankton (planktivorous type). To evaluate the effect of food on intestinal bacterial microbiota, we characterized and compared the intestinal microbiota of these three types of bluegill in terms of community-level physiological profile (CLPP) and genetic structure. The CLPP was analyzed using Biolog MicroPlates (Biolog, Inc., Hayward, CA, USA), and multivariate analysis of variance revealed that the CLPP of intestinal microbiota differed significantly between any pairs of the three types of bluegill. The genetic profiles were analyzed by temperature gradient gel electrophoresis of polymerase chain reaction (PCR)-amplified 16S rDNA fragments, and multidimensional scaling indicated the existence of specific intestinal bacterial structures for both the benthivorous and the planktivorous types. These results suggest that the host's feeding habit can be one factor controlling the intestinal microbiota of fish in the natural environment.

Keywords

Bacterial Community Intestinal Microbiota Benthic Invertebrate Intestinal Bacterium Terrestrial Insect 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We greatly acknowledge S. Gritters and K. Aulwes, Iowa Department of Natural Resources, for fish sampling in Iowa. We thank N. Yamaguchi, C. Sakamoto, F. Maruyama, and other members of Osaka University for their help in the TGGE experiments. We also thank M. Yuma for reviewing an earlier draft of this manuscript, and M. Honjo and M. Ueki for helpful comments and technical assistance. This research was partly supported by the Grant for the Biodiversity Research of the 21st Century COE (A14). The study was also partly supported by the JSPS Grant-in Aid for Basic Research (A) (16207001).

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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Kimiko Uchii
    • 1
    Email author
  • Kazuaki Matsui
    • 2
  • Ryuji Yonekura
    • 3
  • Katsuji Tani
    • 4
  • Takehiko Kenzaka
    • 4
  • Masao Nasu
    • 4
  • Zen'ichiro Kawabata
    • 1
    • 5
  1. 1.Center for Ecological ResearchKyoto UniversityShigaJapan
  2. 2.Laboratory of Environmental Biotechnology, Faculty of EngineeringTohoku Gakuin UniversityTagajoJapan
  3. 3.Gifu Prefectural Research Institute for Freshwater Fish and Aquatic EnvironmentsKakamigaharaJapan
  4. 4.Environmental Science and Microbiology, Graduate School of Pharmaceutical SciencesOsaka UniversitySuitaJapan
  5. 5.Research Institute for Humanity and NatureKamigyo-kuJapan

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