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Microbial Ecology

, Volume 51, Issue 2, pp 242–255 | Cite as

Biodiversity and Biogeography of Fusarium Species from Northeastern North American Asparagus Fields Based on Microbiological and Molecular Approaches

  • Vladimir VujanovicEmail author
  • Chantal Hamel
  • Etienne Yergeau
  • Marc St-ArnaudEmail author
Article

Abstract

Sixteen Fusarium species were recovered from 52 asparagus commercial fields, representing all major ecological (edaphic and climatic) area of asparagus production in the province of Québec, eastern Canada. This study extends our understanding of the geographic range of these species. It also provides climatological and edaphic properties linked to community changes and adaptations. Fusarium oxysporum and F. proliferatum were omnipresent and abundant in all five ecological area under study, whereas F. redolens was less frequently found. Species of Fusarium that produce carmine red pigmentation on potato dextrose agar, i.e., F. acuminatum, F. avenaceum, etc., were common at the northern limit of asparagus production. Abundance of red Fusarium species corresponded with a low isolation frequency of F. proliferatum. Nevertheless, F. proliferatum had a high recovery rate throughout Québec asparagus growing areas, under climatic conditions as cold as those of northern Europe where this species is uncommon in asparagus fields. In the light of these results, redefinition of the geographical distribution of F. proliferatum in asparagus fields is proposed. Intraspecific molecular differences in F. proliferatum and F. oxysporum were detected in the EF-1 alpha sequences and compared with well-characterized strains of North America.

Keywords

Fusarium Fusarium Species Isolation Frequency Vegetative Compatibility Myclobutanil 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This research was financially supported by a CORPAQ grant. The authors are thankful to Dr Wade Elmer, phytopathologist from the Connecticut Agricultural Experiment Station, New Haven, CT, USA, for the loan of US Fusarium specimens as well as for manuscript review and useful comments. We thank Dr. Robert Baayen, from the Plant Protection Service, Mycology Section, Wageningen, The Netherlands, and Dr Lily Tamburic-Ilincic, from the University of Guelph, Guelph, Ontario, Canada, for providing fungal strains. We also thank Jean-Guy Tessier, agronomist from the MAPAQ, for useful professional advice, and Houda Jallal, Université de Montréal, for technical assistance.

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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  1. 1.Institut de Recherche en Biologie VégétaleUniversité de Montréal & Jardin botanique de MontréalMontrealCanada
  2. 2.Environmental Health/Water and Nutrients, Agriculture and Agri-Food CanadaSaskatchewanCanada
  3. 3.Applied Microbiology and Food Science, College of AgricultureUniversity of SaskatchewanCanada

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