Microbial Ecology

, Volume 48, Issue 4, pp 449–462 | Cite as

Halotolerant Aerobic Heterotrophic Bacteria from the Great Salt Plains of Oklahoma

  • T.M. Caton
  • L.R. Witte
  • H.D. Ngyuen
  • J.A. Buchheim
  • M.A. Buchheim
  • M.A. Schneegurt
Microbial Observatories


The Salt Plains National Wildlife Refuge (SPNWR) near Cherokee, Oklahoma, contains a barren salt flat where Permian brine rises to the surface and evaporates under dry conditions to leave a crust of white salt. Rainfall events dissolve the salt crust and create ephemeral streams and ponds. The rapidly changing salinity and high surface temperatures, salinity, and UV exposure make this an extreme environment. The Salt Plains Microbial Observatory (SPMO) examined the soil microbial community of this habitat using classic enrichment and isolation techniques and phylogenetic rDNA studies. Rich growth media have been emphasized that differ in total salt concentration and composition. Aerobic heterotrophic enrichments were performed under a variety of conditions. Heterotrophic enrichments and dilution plates have generated 105 bacterial isolates, representing 46 phylotypes. The bacterial isolates have been characterized phenotypically and subjected to rDNA sequencing and phylogenetic analyses. Fast-growing isolates obtained from enrichments with 10% salt are predominantly from the gamma subgroup of the Proteobacteria and from the low GC Gram-positive cluster. Several different areas on the salt flats have yielded a variety of isolates from the Gram-negative genera Halomonas, Idiomarina, Salinivibrio, and Bacteroidetes. Gram-positive bacteria are well represented in the culture collection including members of the Bacillus, Salibacillus, Oceanobacillus, and Halobacillus.


  1. 1.
    Al-Tai, AM, Ruan, J-S 1994Nocardiopsis halophila sp. nov., a new halophilic actinomycete isolated from soil.Int J System Bacteriol44474478Google Scholar
  2. 2.
    Altschul, SF, Gish, W, Miller, W, Myers, EW, Lipman, DJ 1990Basic local alignment search tool.J Mol Biol215403410CrossRefPubMedGoogle Scholar
  3. 3.
    Antón, J, Rosselló-Mora, R, Rodríguez-Valera, F, Amann, R 2000Extremely halophilic Bacteria in crystallizer ponds from solar salterns.Appl Environ Microbiol6630523057CrossRefPubMedGoogle Scholar
  4. 4.
    Arahal, DR, García, MT, Vargas, C, Cánovas, D, Nieto, JJ, Ventosa, A 2001Chromohalobacter salexigens sp. nov., a moderately halophilic species that includes Halomonas elongata DSM 3043 and ATCC 33174.Int J System Evol Microbiol5114571462Google Scholar
  5. 5.
    Arahal, DR, Márquez, MC, Volcani, BE, Schleifer, KH, Ventosa, A 1999Bacillus marismortui sp. nov., a new moderately halophilic species from the Dead Sea. Int J System Evol Microbiol49521530Google Scholar
  6. 6.
    Arahal, DR, Márquez, MC, Volcani, BE, Schleifer, KH, Ventosa, A 2000Reclassification of Bacillus marismortui as Salibacillus marismortui comb. nov.Int J System Evol Microbiol5015011503Google Scholar
  7. 7.
    Ash, C, Farrow, JAE, Wallbanks, S, Collins, MD 1991Phylogenetic heterogeneity of the genus Bacillus revealed by comparative analysis of small-subunit-ribosomal RNA sequences.Lett Appl Microbiol13202206Google Scholar
  8. 8.
    Atlas, RM 1993Handbook of Microbiological MediaCRC PressBoca Raton, FL64Google Scholar
  9. 9.
    Austin, B 1989A review: novel pharmaceutical compounds from marine bacteria.J Appl Bacteriol67461468PubMedGoogle Scholar
  10. 10.
    Battista, JR 1997Against all odds: the survival strategies of Deinococcus radiodurans.Ann Rev Microbiol51203224Google Scholar
  11. 11.
    Baumgarte, S, Moore, ERB, Tindall, BJ 2001Re-examining the 16S rDNA sequence of Halomonas salina.Int J System Evol Microbiol515153Google Scholar
  12. 12.
    Benlloch, S, Martinez-Murcia, AJ, Rodriquez-Valera, F 1995Sequencing of bacterial and archaeal 16S rRNA genes directly amplified from a hypersaline environment.Syst Appl Microbiol18574581Google Scholar
  13. 13.
    Bozal, N, Monies, MJ, Tudela, E, Guinea, J 2003Characterization of several Psychrobacter strains isolated from Antarctic environments and description of Psychrobacter luti sp. nov. and Psychrobacter fozii sp. nov.Int J Syst Evol5310931100CrossRefGoogle Scholar
  14. 14.
    Brettar, I, Christen, R, Hofle, MG 2003Idiomarina baltica sp. nov., a marine bacterium with a high optimum growth temperature isolated from surface water of the central Baltic Sea.Int J Syst Evol Microbiol53407413CrossRefPubMedGoogle Scholar
  15. 15.
    Castanier, S, Perthuisot, J-P, Rouchy, J-M, Maurin, A, Guelorget, O 1992Halite ooids in Lake Asal, Djibouti: biocrystalline build-ups.Geobios25811821Google Scholar
  16. 16.
    Caton, TM, Witte, LR, Buchheim, JA, Buchheim, MA, Schneegurt, MA (2003) Phenetic and phylogenetic characterization of the prokaryotic assemblage at the Great Salt Plains of Oklahoma. Abs 102nd Gen Mtg Am Soc Microbiol N-321:455–456Google Scholar
  17. 17.
    Chai, LH, Cui, XL, Wang, T, Xu, LH, Jiang, CL (2002) The microbial diversity of Salt Lake Keke in Western China. GenBank no. AY121439Google Scholar
  18. 18.
    Cole, JR, Chai, B, Marsh, TL, Farris, RJ, Wang, Q, Kulam, SA, Chandra, S, McGarrell, DM, Schmidt, TM, Garrity, GM, Tiedje, JM 2003The Ribosomal Database Project (RDP-II): previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy.Nucleic Acids Res31442443CrossRefPubMedGoogle Scholar
  19. 19.
    Csonka, LN 1989Physiological and genetic responses of bacteria to osmotic stress.Microbiol Rev53121147PubMedGoogle Scholar
  20. 20.
    Dobson, SJ, Franzmann, PD (1995). GenBank no. L42618Google Scholar
  21. 21.
    Dobson, SJ, Franzmann, PD 1996Unification of the genera Deleya (Baumann et al. 1983), Halomonas (Vreeland et al. 1980), and Halovibrio (Fendic 1988) and the species Paracoccus halodenitrificans (Robinson and Gibbons 1952) into a single genus, Halomonas, and placement of the genus Zymobacter in the family Halomonadaceae.Int J Syst Bacteriol46550558Google Scholar
  22. 22.
    Donachie, SP, Hou, S, Gregory, TS, Malahoff, A, Alam, M 2003Idiomarina loihiensis sp. nov., a halophilic gamma-Proteobacterium from the Lo’ihi submarine volcano, Hawai’i.Int J Syst Evol Microbiol5318731879CrossRefPubMedGoogle Scholar
  23. 23.
    Edwards, U, Rogall, H, Blöcker, H, Emde, M, Böttger, EC 1989Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA.Nucleic Acids Res1778437853PubMedGoogle Scholar
  24. 24.
    Farrow, JA, Ash, C, Wallbanks, S, Collins, MD 1992Phylogenetic analysis of the genera Planococcus, Marinococcus and Sporosarcina and their relationship to members of the genus Bacillus.FEMS Microbiol Lett72167172CrossRefPubMedGoogle Scholar
  25. 25.
    Fendrich, C 1988Halovibrio variabilis gen. nov. sp. nov., Pseudomonas halophila sp. nov. and a new halophilic aerobic coccoid eubacterium from Great Salt Lake, Utah, USA.Syst Appl Microbiol113643Google Scholar
  26. 26.
    Fu, X, Schmitz, FJ, Tanner, RS 1995Chemical constituents of halophilic facultatively anaerobic bacteria.J Nat Prod5819501954Google Scholar
  27. 27.
    Galinski, EA, Tindall, BJ 1992

    Biotechnological prospects for halophiles and halotolerant micro-organisms.

    Herbert, RASharp, RJ eds. Molecular Biology and Biotechnology of ExtremophilesThomson PressNew Delhi76114
    Google Scholar
  28. 28.
    Garabito, MJ, Márquez, MC, Ventosa, A 1998Halotolerant Bacillus diversity in hypersaline environments.Can J Microbiol4495102CrossRefGoogle Scholar
  29. 29.
    Goto, K, Omura, T, Hara, Y, Sadaie, Y 2000Application of the partial 16S rDNA sequence as an index for rapid identification of species in the genus Bacillus.J Gen Appl Microbiol4618Google Scholar
  30. 30.
    Hao, MV, Kocur, M, Komagata, K 1984Marinococcus gen. nov., a new genus for motile cocci with meso-diaminopimelic acid in the cell wall, and Marinococcus albus sp. nov. and Marinococcus halophilus (Novitskyand Kushner) comb. nov.J Gen Appl Microbiol30449459Google Scholar
  31. 31.
    Henis, Y, Eren, J 1963Preliminary studies on the microflora of a highly saline soil.Can J Microbiol9902904Google Scholar
  32. 32.
    Heyndrickx, M, Lebbe, L, Kersters, K, De Vos, P, Forsyth, C, Logan, NA 1998Virgibacillus: a new genus to accommodate Bacillus pantothenticus (Proom and Knight 1950). Emended description of Virgibacillus pantothenticus.Int J Syst Bacteriol4899106Google Scholar
  33. 33.
    Heyrman, J, Logan, NA, Busse, H-J, Balcaen, A, Lebbe, L, Rodriguez-Diaz, M, Swings, J, De Vos, P 2003Virgibacillus carmonensis sp. nov., Virgibacillus necropolis sp. nov. and Virgibacillus picturae sp. nov., three novel species isolated from deteriorated mural paintings, transfer of the species of the genus Salibacillus to Virgibacillus, as Virgibacillus marismortui comb. nov. and Virgibacillus salexigens comb. nov., and emended description of the genus Virgibacillus.Int J Syst Evol Microbiol53501511CrossRefPubMedGoogle Scholar
  34. 34.
    Heyrman, J, Swings, J 200116S rDNA sequence analysis of bacterial isolates from biodeteriorated mural paintings in the Servilia tomb (Necropolis of Carmona, Seville, Spain).Syst Appl Microbiol24417422PubMedGoogle Scholar
  35. 35.
    Huang, C-Y, Garcia, J-L, Patel, BKC, Cayol, J-L, Baresi, L, Mah, RA 2000Salinivibrio costicola subsp. vallismortis subsp. nov., a halotolerant facultative anaerobe from Death Valley, and emended description of Salinivibrio costicola.Int J Syst Evol Microbiol50615622PubMedGoogle Scholar
  36. 36.
    Humayoun, SB, Bano, N, Hollibaugh, JT 2003Depth distribution of microbial diversity in Mono Lake, a meromictic soda lake in California.Appl Environ Microbiol6910301042CrossRefPubMedGoogle Scholar
  37. 37.
    Ivanova, EP, Romanenko, LA, Chun, J, Matte, MH, Matte, GR, Mikahailov, VV, Svetashev, VI, Huq, A, Maugel, T, Colwell, RR 2000Idiomarina gen. nov., comprising novel indigenous deep-sea bacteria from the Pacific Ocean, including descriptions of two species, Idiomarina abyssalis sp. nov. and Idiomarina zobelli sp. nov.Int J Syst Evol Microbiol50901907PubMedGoogle Scholar
  38. 38.
    Javor, B 1989Hypersaline Environments: Microbiology and BiogeochemistrySpringer-VerlagNew York77100Google Scholar
  39. 39.
    Johnson, KS 1980Guidebook for Geologic Field Trips in Oklahoma. Book II: Northwest OklahomaUniversity of OklahomaNorman, OK,1315Google Scholar
  40. 40.
    Jones, BE, Grant, WD, Duckworth, AW, Owenson, GG 1998Microbial diversity of soda lakes.Extremophiles2191200CrossRefPubMedGoogle Scholar
  41. 41.
    Juni, E, Heym, GA 1986Psychrobacter immobilis gen. nov., sp. nov.: genospecies composed of Gram-negative, aerobic, oxidase-positive coccobacilli.Int J Syst Bacteriol36388391Google Scholar
  42. 42.
    Kamekura, M, Seno, Y 1990A halophilic extracellular protease from a halophilic archaebacterium strain.Biochem Cell Biol68352359PubMedGoogle Scholar
  43. 43.
    Kushnir, J 1981Formation and early diagenesis of varved evaporite sediments in a coastal hypersaline pool.J Sed Petrol5111931203Google Scholar
  44. 44.
    Litchfield, CD 1998Survival strategies for microorganisms in hypersaline environments and their relevance to life on early Mars.Meteor Planet Sci33813819Google Scholar
  45. 45.
    Litchfield, CD, Gillevet, PM 2002Microbial diversity and complexity in hypersaline environments: a preliminary assessment.J Ind Microbiol Biotechnol284855Google Scholar
  46. 46.
    Litchfield, CD, Irby, A, Vreeland, RH 1998

    The microbial ecology of solar salt plants.

    Oren, A eds. Microbiology and Biogeochemistry of Hypersaline EnvironmentsCRC PressBoca Raton, FL 3952
    Google Scholar
  47. 47.
    Lu, J, Nogi, Y, Takami, H 2001Oceanobacillus iheyensis gen. nov., sp. nov., a deep-sea extremely halotolerant and alkaliphilic species isolated from a depth of 1050 m on the Iheya Ridge.FEMS Microbiol Lett205291297CrossRefPubMedGoogle Scholar
  48. 48.
    Maas, EW, Kodama, M, Thiele, JH, Bennasar, A, Brooks, HJL, Moore, ERB (1997) Phylogenetic characterization of paralytic shellfish toxin (PST) producing bacteria isolated from marine algal cultures. GenBank no. AJ000648Google Scholar
  49. 49.
    Maddison, DR, Maddison, WP (2000) MacClade 4: Analysis of Phylogeny and Character Evolution. Sinauer Associates, Sunderland, MAGoogle Scholar
  50. 50.
    Manachini, PL, Fortina, MG, Levati, L, Parini, C 1998Contribution to phenotypic and genotypic characterization of Bacillus licheniformis and description of new genomovars.Sys Appl Microbiol21520529Google Scholar
  51. 51.
    Mata, JA, Martínez-Cánovas, J, Quesada, E, Béjar, V 2002A Halomonas detailed phenotypic characterization of the type strains of species.Syst Appl Microbiol25360375PubMedGoogle Scholar
  52. 52.
    Mellado, E, Moore, ERB, Nieto, JJ, Ventosa, A 1995Phylogenetic inferences and taxonomic consequences of 16S ribosomal DNA sequence comparison of Chromohalobacter marismortui, Volcaniella eurihalina, and Deleya salina and reclassification of V. eurihalina as Halomonas eurihalina comb. nov.Int J Syst Bacteriol45712716PubMedGoogle Scholar
  53. 53.
    Mellado, E, Moore, ERB, Nieto, JJ, Ventosa, A 1996Analysis of 16S rRNA gene sequences of Vibrio costicola strains: description of Salinivibrio costicola gen. nov., comb. nov. Int J Syst Bacteriol46817821PubMedGoogle Scholar
  54. 54.
    Miller, RV 1992


    Luria, S eds. Encyclopedia of MicrobiologyAcademic PressSan Diego509517
    Google Scholar
  55. 55.
    Miller, RV 2000

    recA: the gene and its protein product.

    Luria, S eds. Encyclopedia of MicrobiologyAcademic PressSan Diego4354
    Google Scholar
  56. 56.
    Miller, RV, Kokjohn, TA 1990General microbiology of recA: environmental and evolutionary significance.Ann Rev Microbiol44365394Google Scholar
  57. 57.
    Nielsen, P, Rainey, FA, Outtrup, H, Priest, FG, Fritze, D 1994Comparative 16S rDNA sequence analysis of some alkaliphilic bacilli and the establishment of a sixth rRNA group within the genus Bacillus.FEMS Microbiol Lett1176166CrossRefGoogle Scholar
  58. 58.
    Ochsenreiter, T, Pfeifer, F, Schleper, C 2002Diversity of Archaea in hypersaline environments characterized by molecular-phylogenetic and cultivation studies.Extremophiles6267274CrossRefPubMedGoogle Scholar
  59. 59.
    Oren, A 1990Estimation of the contribution of halobacteria to the bacterial biomass and activity in solar salterns by the use of bile salts.FEMS Microb Ecol734148CrossRefGoogle Scholar
  60. 60.
    Oren, A 1999Bioenergetic aspects of halophilism.Microbiol Molec Biol Rev63334348Google Scholar
  61. 61.
    Palmisano, MM, Nakamura, LK, Duncan, KE, Istock, CA, Cohan, FM 2001Bacillus sonorensis sp. nov., a close relative of Bacillus licheniformis, isolated from soil in the Sonoran Desert, Arizona.Int J System Evol Microbiol5116711679Google Scholar
  62. 62.
    Prado, B, Del Moral, A, Quesada, E, Ríos, R, Monteoliva-Sanchez, M, Campos, V, Ramos-Cormenzana, A 1991Numerical taxonomy of moderately halophilic Gram-negative rods isolated from the Salar de Atacama, Chile.Syst Appl Microbiol14275281Google Scholar
  63. 63.
    Quesada, E, Bejar, V, Valderrama, MJ, Ventosa, A, Ramos-Cormenzana, A 1985Isolation and characterization of moderately halophilic nonmotile rods from different saline habitats.Microbiología18996Google Scholar
  64. 64.
    Quesada, E, Ventosa, A, Rodriguez-Valera, F, Megias, L, Ramos-Cormenzana, A 1983Numerical taxonomy of moderately halophilic Gram-negative bacteria from hypersaline soils.J Gen Microbiol12926492657Google Scholar
  65. 65.
    Reed, JE (1982) Preliminary projections of the effects of chloride-control structures on the quaternary aquifer at the Great Salt Plains, Oklahoma, US Geological Survey, Water-Resources Investigations 80–120, Oklahoma City, OklahomaGoogle Scholar
  66. 66.
    Rivadeneyra, MA, Delgado, G, Soriano, M, Ramos-Cormenzana, A, Delgado, R 1983Biomineralization of carbonates by Marinococcus albus and Marinococcus halophilus isolated from the Salar de Atacama (Chile).Curr Microbiol395357Google Scholar
  67. 67.
    Rodriguez-Valera, F 1992Biotechnological potential of halobacteria.Biochem Soc Symp58135147PubMedGoogle Scholar
  68. 68.
    Rodriguez-Valera, F, Riuz-Berraquero, F, Ramos-Cormenzana, A 1980Isolation of extremely halophilic bacteria able to grow in defined organic media with single carbon sources.J Gen Microbiol119535538Google Scholar
  69. 69.
    Rodriguez-Valera, F, Ventosa, A, Juez, G, Imhoff, JF 1985Variation of environmental features and microbial populations with salt concentrations in a multi-pond saltern.Microb Ecol11107111Google Scholar
  70. 70.
    Rothschild, LJ 1999

    Microbes and radiation.

    Seckbach, J eds. Enigmatic Microorganisms and Life in Extreme EnvironmentsKluwer Academic PublishersDordrecht551562
    Google Scholar
  71. 71.
    Rothschild, LJ, Giver, LJ, White, MR, Mancinelli, RL 1994Metabolic activity of microorganisms in evaporites.J Phycol30431438CrossRefPubMedGoogle Scholar
  72. 72.
    Schlesner, H, Lawson, PA, Collins, MD, Weiss, N, Wehmeyer, U, Volker, H, Thomm, M 2001Filobacillus milensis gen. nov., sp. nov., a new halophilic spore-forming bacterium with Orn-D-Glu-type peptidoglycan.Int J Syst Evol Microbiol51425431PubMedGoogle Scholar
  73. 73.
    Schneegurt, MA, Caton, TM, Nguyen, HD, Potter, A, Henley, WJ (2002) Initial characterization of the microbial community of the Great Salt Plains of Oklahoma. Abs 102nd Gen Mtg Am Soc Microbiol N-141:329Google Scholar
  74. 74.
    Spring, S, Ludwig, W, Marquez, MC, Ventosa, A, Schleifer, K-H 1996Halobacillus gen. nov., with descriptions of Halobacillus litoralis sp. nov. and Halobacillus trueperi sp. nov., and transfer of Sporosarcina halophila to Halobacillus halophilus comb. nov.Int J Syst Bacteriol46492496Google Scholar
  75. 75.
    Suemori, A, Nakajima, K (1998) Isolation and characterization of alkaliphilic Bacillus sp. assimilating inulin: Bacillus alcaliinulinus sp. nov. GenBank no. AB018595Google Scholar
  76. 76.
    Swofford, DL (2002) PAUP*: Phylogenetic Analysis Using Parsimony, Version 4.0b10. Sinauer Associates, Sunderland, MAGoogle Scholar
  77. 77.
    Takami, H, Krulwich, TA 2000Reidentification of facultatively alkaliphilic Bacillus firmus OF4 as Bacillus pseudofirmus OF4.Extremophiles41922PubMedGoogle Scholar
  78. 78.
    Takami, H, Takaki, Y, Uchiyama, I 2002Genome sequence of Oceanobacillus iheyensis isolated from the Iheya Ridge and its unexpected adaptive capabilities to extreme environments.Nucleic Acids Res3039273935CrossRefPubMedGoogle Scholar
  79. 79.
    Tanner, RS, Poynter, NL, Mandelco, L, Woese, CR (1994) Vibrio aspartigenicus, sp. nov., a halophilic, facultatively anaerobic eubacterium that produces aspartic acid. GenBank Taxon ID 36872Google Scholar
  80. 80.
    Torsvik, V, Goksøyr, J, Daae, FL 1990High diversity of DNA of soil bacteria.Appl Environ Microbiol56782787Google Scholar
  81. 81.
    Valderamma, MJ, Quesada, E, Bejar, V, Ventosa, A, Gutierrez, MC, Ruiz-Berraquero, F, Ramos-Cormenzanna, A 1993Deleya salina sp. nov., a moderately halophilic Gram-negative bacterium.Int J Syst Bacteriol41377384Google Scholar
  82. 82.
    Venkateswaran, K, Hattori, N, La Duc, MT, Kern, R 2003ATP as a biomarker of viable microorganisms in clean-room facilities.J Microbiol Methods52367377CrossRefPubMedGoogle Scholar
  83. 83.
    Ventosa, A, Quesada, E, Rodriguez-Valera, F, Ruiz-Berraquero, F, Ramos-Cormenzana, A 1982Numerical taxonomy of moderately halophilic Gram-negative rods.J Gen Microbiol12819551968Google Scholar
  84. 84.
    Vreeland, RH, Martin, EL 1980Growth characteristics, effects of temperature, and ion specificity of the halotolerant bacterium Halomonas elongata.Can J Microbiol26746752Google Scholar
  85. 85.
    Waino, M, Tindall, BJ, Ingvorsen, K 2000Halorhabdus utahensis gen. nov., sp. nov., an aerobic, extremely halophilic member of the Archaea from Great Salt Lake, Utah.Int J Syst Evol Microbiol50183190PubMedGoogle Scholar
  86. 86.
    Yoon, JH, Kang, KH, Park, YH 2003Halobacillus salinus sp. nov., isolated from a salt lake on the coast of the East Sea in Korea.Int J Syst Evol Microbiol53687693CrossRefPubMedGoogle Scholar
  87. 87.
    Yoon, JH, Kim, IG, Kang, KH, Oh, TK, Park, YH 2003Bacillus marisflavi sp. nov. and Bacillus aquaemaris sp. nov. isolated from seawater of a tidal flat of the Yellow Sea in Korea.Int J Syst Evol Microbiol5312971303CrossRefPubMedGoogle Scholar
  88. 88.
    Yoon, JH, Kim, IK, Kang, KH, Park, YH (2002) Bacillus hwajinpoensis sp. nov. and Bacillus baekryungensis sp. nov., new members of Bacillus rRNA group 6 isolated from seawater of the East Sea and the Yellow Sea in Korea. GenBank no. AF541965Google Scholar
  89. 89.
    Yoon, JH, Lee, KC, Kho, YH, Kang, KH, Kim, CJ, Park, YH 2002bHalomonas alimentaria sp. nov., isolated from jeotgal, a traditional Korean fermented seafood.Int J Syst Evol Microbiol52123130Google Scholar
  90. 90.
    Yumoto, I, Yamaga, S, Sogabe, Y, Nodasaka, Y, Matsuyama, H, Nakajima, K, Suemori, A 2003Bacillus krulwichiae sp. nov., a halotolerant obligate alkaliphile that utilizes benzoate and m-hydroxybenzoate.Int J Syst Evol Microbiol5315311536CrossRefPubMedGoogle Scholar
  91. 91.
    Zhang, W, Ma, Y, Mao, W (2000) Unidentified Hailaer soda lake bacterium T10. GenBank no. AF275707Google Scholar

Copyright information

© Springer Science+Business Media, Inc. 2004

Authors and Affiliations

  • T.M. Caton
    • 1
  • L.R. Witte
    • 1
  • H.D. Ngyuen
    • 1
  • J.A. Buchheim
    • 2
  • M.A. Buchheim
    • 2
  • M.A. Schneegurt
    • 1
  1. 1.Department of Biological SciencesWichita State UniversityWichitaUSA
  2. 2.Department of Biological SciencesUniversity of TulsaTulsaUSA

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