Screening for Putative PGPR to Improve Establishment of the Symbiosis Lactarius deliciosus-Pinus sp.
- 468 Downloads
A screening for plant growth promoting rhizobacteria (PGPR) was carried out in the mycorrhizosphere of wild populations of Pinus pinea and P. pinaster, and in the mycosphere of associated Lactarius deliciosus. A total of 720 bacteria were isolated, purified, and grouped by morphological criteria. Fifty percent of the isolates were selected and tested for aminocyclopropanecarboxylic acid (ACC) degradation, auxin and siderophore production, and phosphate solubilization. Thirty eight percent of the isolates showed at least one of the evaluated activities. Nutrient-related traits were associated with P. pinaster, whereas hormone production traits predominated in P. pinea. These activities were found mostly in Gram positive isolates. After PCR-RAPDs (random amplified polymorphic DNA) analysis, 10 groups appeared with 85% similiarity when considering all isolates, indicating the low diversity in the system. One strain of each group was identified by 16S rDNA sequencing. Our results suggest that P. pinaster selects for mycorrhizosphere bacteria that mobilize nutrients, whereas P. pinea selects for bacteria that have the capacity to increase root growth via production of plant growth regulators.
KeywordsPlant Growth Promote Rhizobacteria Siderophore Production Lactarius Tree Nursery Bioedit Sequence Alignment Editor
This study was financed by project FEDER 1FD97-1441. We thank Linda Hamalainen for editorial help.
- 3.Bowen GD, Rovira AD (1999) The rhizosphere and its management to improve plant growth. Adv Agron 66:1–103Google Scholar
- 4.Burdman S, Jurkevith E, Okon Y (2000) Recent advances in the use of plant growth promoting rhizobacteria (PGPR) in agriculture. Microbial Interations in Agriculture and Forestry 2:229–250Google Scholar
- 5.Cattelan AJ, Hartel PG, Fuhrmann JJ (1999) Screeening for plant growth-promoting rhizobacteria to promote early soybean growth. Soil Sci Soc Am J 63:1670–1680Google Scholar
- 9.Enebak SA, Wei G, Kloepper JW (1997) Effects of plant growth-promoting rhizobacteria on loblolly and slash pine seedlings. Forest Sci. 44:139–144Google Scholar
- 10.Frey-Klett P, Pierrat JC, Garbaye J (1997) Location and survival of Mycorrhiza helper Pseudomonas fluorescens during establishment of ectomycorrhizal symbiosis between Laccaria bicolour and Douglas fir. Appl Environ Microbiol 63:139–144Google Scholar
- 11.Garbaye J (1994) Helper bacteria: a new dimension to the mycorrhizal symbiosis. New Phyto. 128:197–210Google Scholar
- 12.Glick BR, Karaturovic DM, Newell PC (1995) A novel procedure for rapid isolation of plant growth promoting pseudomonads. Can J Microbiol 41:533–536Google Scholar
- 13.Gutierrez Mañero FJ, Acero N, Lucas JA, Probanza A (1996) The influence of native rhizobacteria on European alder [Alnus glutinosa (L.) Gaertn.] growth. II. Characterization of growth promoting and growth inhibiting strains. Plant Soil 182:67–74Google Scholar
- 15.Gutierrez Manero FJ, Ramos B, Lucas Garcia JA, Probanza A, Barrientos ML (2002) Systemic induction of terpenic compounds in D. lanata. J Plant Physiol 160:105–113Google Scholar
- 18.Kloepper JW, Scrhoth MN, Miller TD (1980) Effects of rhizosphere colonization by plant growth-promoting rhizobacteria on potato plant development and yield. Phytopathology 70:1078–1082Google Scholar
- 21.Lynch JM, (1990) The rhizosphere. Wiley-Interscience, Chichester, EnglandGoogle Scholar
- 23.Marten P, Brueckner S, Berg G (2001) Biological plant protection using rhizobacteria–an environmentally friendly alternative for biological control of soilborne and seedborne phytopathogenic fungi. Gesunde Prlanzen 53:224–234Google Scholar
- 25.Probanza A, Mateos JL, Lucas JA, Ramos B, de Felipe MR, Gutierrez Manero FJ (2001) Effects of inoculation with PGPR Bacillus and Pisolitus tinctorius on Pinus pinea L. growth, bacterial rhizosphere colonization and mycorrhizal infection. Microbial Ecol 41:140–148Google Scholar
- 28.Ramos Solano, B, Pereyra de la Iglesia, MT, Probanza, A, Lucas Garcia, JA, Megiás, M, Gutíerrez Mañero, FJ (2003) Screening for PGPR in the rhizosphere of Cistus ladanifer to improve reforestation of degraded mediterranean ecosystems. Plant Soil (in press)Google Scholar
- 29.Ramos B, Pozuelo JM, Acero N, Gutierrez Mañero FJ (1998). Seasonal variations of Bacillus isolated from the rhizosphere of Elaeagnus angustifolia L. Orsis 13:7–16Google Scholar
- 33.Shannon CE, Weaver V (1949) The Mathematical Theory of Communication. University of Illinois Press, Urbana, IL, 117 PpGoogle Scholar
- 34.Smith, SE, Read, DJ (1997) Mycorrhizal Symbiosis. Academic Press, San Diego, USAGoogle Scholar
- 38.Wayne LG, Brenner DJ, Colwell RR, Grimont PAD, Kandler O, Kirchevsdy MI, Moore LH, Moore WEC, Murray RGE, Stackerbrandt E, Starr MP, Turper HG (1987) Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Sys Bacteriol 37:463–464Google Scholar