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Microbial Ecology

, Volume 50, Issue 1, pp 82–89 | Cite as

Screening for Putative PGPR to Improve Establishment of the Symbiosis Lactarius deliciosus-Pinus sp.

  • J. Barriuso
  • M. T. Pereyra
  • J. A. Lucas García
  • M. Megías
  • F. J. Gutierrez Mañero
  • B. Ramos
Article

Abstract

A screening for plant growth promoting rhizobacteria (PGPR) was carried out in the mycorrhizosphere of wild populations of Pinus pinea and P. pinaster, and in the mycosphere of associated Lactarius deliciosus. A total of 720 bacteria were isolated, purified, and grouped by morphological criteria. Fifty percent of the isolates were selected and tested for aminocyclopropanecarboxylic acid (ACC) degradation, auxin and siderophore production, and phosphate solubilization. Thirty eight percent of the isolates showed at least one of the evaluated activities. Nutrient-related traits were associated with P. pinaster, whereas hormone production traits predominated in P. pinea. These activities were found mostly in Gram positive isolates. After PCR-RAPDs (random amplified polymorphic DNA) analysis, 10 groups appeared with 85% similiarity when considering all isolates, indicating the low diversity in the system. One strain of each group was identified by 16S rDNA sequencing. Our results suggest that P. pinaster selects for mycorrhizosphere bacteria that mobilize nutrients, whereas P. pinea selects for bacteria that have the capacity to increase root growth via production of plant growth regulators.

Keywords

Plant Growth Promote Rhizobacteria Siderophore Production Lactarius Tree Nursery Bioedit Sequence Alignment Editor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Aknowledgments

This study was financed by project FEDER 1FD97-1441. We thank Linda Hamalainen for editorial help.

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Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  • J. Barriuso
    • 1
  • M. T. Pereyra
    • 1
  • J. A. Lucas García
    • 1
  • M. Megías
    • 2
  • F. J. Gutierrez Mañero
    • 1
  • B. Ramos
    • 1
  1. 1.Facultad FarmaciaUniversidad San Pablo CEUMadridSpain
  2. 2.Facultad FarmaciaUniversidad de SevillaSevillaSpain

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