Microbial Ecology

, Volume 49, Issue 3, pp 451–460 | Cite as

Biogeographic and Quantitative Analyses of Abundant Uncultivated γ-Proteobacterial Clades from Marine Sediment

  • J.P. BowmanEmail author
  • S.A. McCammon
  • A.L. Dann


16S rRNA gene-based molecular analyses revealed the presence of several large and so far uncultivated clades within class γ-Proteobacteria, designated γ-proteobacterial marine sediment (GMS) clades 1 to 4, in marine sediment. The GMS clades appear only indigenous to marine sediment and so far have an unknown functionality. SYBR Green–based real-time PCR analyses using GMS clade-specific primers indicated GMS clades were a significant part of the bacterial community (0.3–8.7% of total 16S rRNA genes) in both polar and temperate marine sediment samples. Univariate statistical analyses indicated that GMS clade communities were indistinguishable in two temperate coastal sediment samples even though these possessed very different mean grain sizes, organic contents, and organic loading rates. GMS clade communities were slightly different (p < 0.05) between polar and temperate sites, suggesting that psychrophilic adaptation among GMS clade taxa corresponds only to subtle phylogenetic differences. Similar levels of difference were also observed through a sediment core reflecting that through the sediment core history, which spanned ∼3000 years, GMS clonal diversity shifted only marginally.


Clone Library Marine Sediment Sample Surface Marine Sediment Maximum Likelihood Distance Core 10GC01 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



Support for this study came from University of Tasmania IRGS funding (grant no. B0013108) and from the Australian Research Council funds (grant no. F09905711). We thank Andrew Bissett for the Tasmanian sediment DNA samples and Catriona MacLeod for access to the Primer 5 program.


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Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  1. 1.School of Agricultural ScienceUniversity of TasmaniaHobartAustralia

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