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Microbial Ecology

, Volume 47, Issue 2, pp 159–163 | Cite as

Extreme Spore UV Resistance of Bacillus pumilus Isolates Obtained from an Ultraclean Spacecraft Assembly Facility

  • L. Link
  • J. Sawyer
  • K. Venkateswaran
  • W. Nicholson
Article

Abstract

Recent environmental microbial sampling of the ultraclean Spacecraft Assembly Facility at NASA Jet Propulsion Laboratory (JPL-SAF) identified spores of Bacillus pumilus as major culturable bacterial contaminants found on and around spacecraft. As part of an effort to assess the efficacy of various spacecraft sterilants, purified spores of 10 JPL-SAF B. pumilus isolates were subjected to 254-nm UV and their UV resistance was compared to spores of standard B. subtilis biodosimetry strains. Spores of six of the 10 JPL-SAF isolates were significantly more resistant to UV than the B. subtilis biodosimetry strain, and one of the JPL-SAF isolates, B. pumilus SAFR-032, exhibited the highest degree of spore UV resistance observed by any Bacillus spp. encountered to date.

Keywords

Bacillus Pumilus Type Strain ATCC Planetary Protection H2O2 Resistance Liquid H2O2 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

The authors thank Michael Kempf for sharing data prior to publication. This work was supported by contract No. 1238194 from NASA-JPL and grant NCC2-1342 from the NASA Exobiology program to W.L.N., and by a University of Arizona-NASA Space Grant to J.S. A portion of this work (K.V.) was carried out at the Jet Propulsion Laboratory, California Institute of Technology, under contract with NASA.

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Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • L. Link
    • 1
  • J. Sawyer
    • 1
  • K. Venkateswaran
    • 2
  • W. Nicholson
    • 1
  1. 1.Department of Veterinary Science and MicrobiologyUniversity of Arizona, Tucson, AZ 85721USA
  2. 2.Biotechnology and Planetary Protection GroupNASA Jet Propulsion Laboratories, California Institute of Technology, Pasadena, CA, 91109USA

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