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Microbial Ecology

, Volume 47, Issue 1, pp 9–17 | Cite as

Abundance, Distribution, and Diversity of Viruses in Alkaline, Hypersaline Mono Lake, California

  • S. Jiang
  • G. Steward
  • R. Jellison
  • W. Chu
  • S. Choi
Article

Abstract

Mono Lake is a large (180 km2), alkaline (pH ~10), moderately hypersaline (70–85 g kg−1) lake lying at the western edge of the Great Basin. An episode of persistent chemical stratification (meromixis) was initiated in 1995 and has resulted in depletion of oxygen and accumulation of ammonia and sulfide beneath the chemocline. Although previous studies have documented high bacterial abundances and marked seasonal changes in phytoplankton abundance and community composition, there have been no previous reports on the occurrence of viruses in this unique lake. Based on the high concentrations and diversity of microbial life in this lake, we hypothesized that planktonic viruses are also abundant and diverse. To examine the abundance and distribution of viruses and bacteria, water samples were collected from four stations along 5 to 15 vertical depths at each station. Viral abundance ranged from 1 × 108 to 1 × 109 mL−1, among the highest observed in any natural aquatic system examined so far. Increases (p < 0.1) in viral densities were observed in the anoxic bottom water at multiple stations. However, regression analysis indicated that viral abundance could not be predicted by any single environmental parameter. Pulsed field gel electrophoresis revealed a diverse viral community in Mono Lake with genome sizes ranging from ~14 to >400 kb with most of the DNA in the 30 to 60 kb size range. Cluster analysis grouped the anoxic bottom-water viral community into a unique cluster differentiating it from surface and mid-water viral communities. A hybridization study using an indigenous viral isolate as a probe revealed an episodic pattern of temporal phage distribution with strong niche stratification between oxic and anoxic waters.

Keywords

Phytoplankton Bacterial Abundance Brine Shrimp Soda Lake Anoxic Water 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

We thank the entire Mono Lake Microbial Observatory Research Team (PIs: J. Hollibaugh and S. Joye, U Georgia; J. Zehr, UC Santa Cruz) for close collaboration and providing sampling and data support for this project. Special thanks also go to Dan Dawson for providing logistic support at the Sierra Nevada Aquatic Research Laboratory and Sandra Roll for providing in situ physical and chemical data. The funding for this project was provided by UC Multi-campus Research Incentive Fund 02-T-MRIF-09-0023, NSF awards DEB-01-30528, DEB-01-29174, DEB-01-29160, and MCB 99-77886, MCB-99-77901.

References

  1. 1.
    Adams MH (1959) Bacteriophages Interscience, New YorkGoogle Scholar
  2. 2.
    Cloern, JE, Cole, BE, Oremland, RS 1983Seasonal changes in the chemistry and biology of a meromictic lake (Big Soda Lake, Nevada, USA).Hydrobiologia105195206Google Scholar
  3. 3.
    Connell, TL, Joye, SB, Miller, LG, Oremland, RS 1997Bacterial oxidation of methyl bromide in Mono Lake, California.Environ Sci Technol3114891495Google Scholar
  4. 4.
    Dana, GL, Lenz, PH 1986Effects of increasing salinity on an Artemina population from Mono Lake, California.Ecologia68428436Google Scholar
  5. 5.
    Duckworth, AW, Grant, WD, Jones, BE, Van Steenbergen, R 1996Phylogenetic diversity of soda lake alkaliphiles.FEMS Microbiol Ecol19181191Google Scholar
  6. 6.
    Fuhrman, JA 1999Marine viruses and their biogeochemical and ecological effects.Nature (London)399541548CrossRefGoogle Scholar
  7. 7.
    Guixa-Boixareu, N, Calderon-Paz, JI, Heldal, M, Bratbak, G, Pedros-Alio, C 1996Viral lysis and bacterivory as prokaryotic loss factors along a salinity gradient.Aquat Microb Ecol11215227Google Scholar
  8. 8.
    Hollibaugh, JT, Wong, PS, Bano, N, Pak, SK, Prager, EM, Orrego, C 2001Stratification of microbial assemblages in Mono Lake, California, and response to a mixing event.Hydrobiologia4664560CrossRefGoogle Scholar
  9. 9.
    Humayoun, SB, Bano, N, Hollibaugh, JT 2003Depth distribution of microbial diversity in mono lake, a meromictic soda lake in California.Appl Environ Microbiol6910301042CrossRefPubMedGoogle Scholar
  10. 10.
    Jellison, R, Melack, JM 1993Algal photosynthetic activity and its response to meromixis in hypersaline Mono Lake, California.Limnol Oceanogr38818837Google Scholar
  11. 11.
    Jiang, S, Fu, W, Chu, W, Fuhrman, JA 2003The vertical distribution and diversity of marine bacteriophage at a station off southern CaliforniaMicrob Ecol45399410PubMedGoogle Scholar
  12. 12.
    Joye, SB, Connell, TL, Miller, LG, Oremland, RS, Jellison, RS 1999Oxidation of ammonia and methane in an alkaline, saline lake.Limnol Oceanogr44178188Google Scholar
  13. 13.
    Lenz, PH, Cooper, SD, Melack, JM, Windler, DW 1986Spatial and temporal distribution patterns of three trophic levels in a saline lake.J Plankton Res810511064Google Scholar
  14. 14.
    Lewin, RA, Krienitz, L, Goericke, R, Takeda, H, Hepperle, D 2000 Picocystis salinarum gen. et sp. nov. (Chlorophyta): A new picoplanktonic green alga.Phycologia39560565Google Scholar
  15. 15.
    Maranger, P, Bird, DF 1996High concentrations of viruses in the sediments of Lake Gilbert, Quebec.Microb Ecol31141151Google Scholar
  16. 16.
    Maranger, R, Bird, DF, Juniper, SK 1994Viral and bacterial dynamics in Arctic sea ice during the spring algal bloom near Resolute, N.W.T., Canada.Mar Ecol Pro Ser111121127Google Scholar
  17. 17.
    Mason, DT 1967Limnology of Mono Lake, California.Univ Calif Publ Zool831110Google Scholar
  18. 18.
    Melack, JM, Jellison, R 1998Limnological conditions in Mono Lake: Contrasting monomixis and meromixis in the 1990s.Hydrobiologia3842139CrossRefGoogle Scholar
  19. 19.
    Murray, AG, Eldridge, PM 1994Marine viral ecology: Incorporation of bacteriophage into the microbial planktonic food web paradigm.J Plankton Res16627641Google Scholar
  20. 20.
    Noble, RT, Fuhrman, JA 1998Use of SYBR Green I for rapid epifluorescence counts of marine viruses and bacteria.Aquat Microb Ecol14113118Google Scholar
  21. 21.
    Oremland, RS, Miller, LG, Culbertson, CW, Robinson, SW, Smith, RL, Lovley, D, Whiticar, ML, King, GM, Kene, RP, Iversen, N, Sargent, M 1993Aspects of the biogeochemistry of methane in Mono Lake and the Mono Basin of California.Oremland, RS eds. Biogeochemistry of Global Change.Chapman & HallNew YorkGoogle Scholar
  22. 22.
    Oremland, RS, Miller, LG, Writicar, MJ 1987Sources and flux of natural gases from Mono Lake, California.Geochim Cosmochim Acta5129152929CrossRefGoogle Scholar
  23. 23.
    Reisser, W 1993Viruses and virus-like particles of freshwater and marine eukaryotic algae: A review.Archiv Protist143257265Google Scholar
  24. 24.
    Steward, GF 2001Fingerprinting viral assemblages by pulsed field gel electrophoresis.Paul, JH eds. Methods in Microbiology.Academic PressNew York85104Google Scholar
  25. 25.
    Steward, GF, Montiel, JL, Azam, F 2000Genome size distributions indicate variability and similarities among marine viral assemblages from diverse environments.Limnol Oceanogr4516971706Google Scholar
  26. 26.
    Weinbauer, MG, Hofle, MG 1998Significance of viral lysis and flagellate grazing as factors controlling bacterioplankton production in a eutrophic lake.Appl Environ Microbiol64431438Google Scholar
  27. 27.
    Wommack, KE, Colwell, RR 2000Virioplankton: Viruses in aquatic ecosystems.Microbiol Mol Biol Rev6469114CrossRefPubMedGoogle Scholar
  28. 28.
    Wommack, KE, Hill, RT, Colwell, RR 1995A simple method for the concentration of viruses from natural water samples.J Microbiol Methods225767CrossRefGoogle Scholar
  29. 29.
    Wommack, KE, Ravel, J, Hill, RT, Chun, J, Colwell, RR 1999Population dynamics of Chesapeake Bay virioplankton: Total-community analysis by pulsed-field gel electrophoresis.Appl Environ Microbiol65231240PubMedGoogle Scholar
  30. 30.
    Wommack, KE, Ravel, J, Hill, RT, Colwell, RR 1999Hybridization analysis of Chesapeake Bay virioplankton Appl Environ Microbiol65241250PubMedGoogle Scholar

Copyright information

© Springer-Verlag 2003

Authors and Affiliations

  • S. Jiang
    • 1
  • G. Steward
    • 2
  • R. Jellison
    • 3
  • W. Chu
    • 1
  • S. Choi
    • 1
  1. 1.Environmental Analysis and DesignUniversity of California, Irvine, CA 92696USA
  2. 2.Department of OceanographyUniversity of Hawaii at Manoa, Honolulu, HI 96822USA
  3. 3.Marine Science InstituteUniversity of California, Santa Barbara, CA 93106USA

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