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Microbial Ecology

, Volume 47, Issue 3, pp 243–251 | Cite as

Identification of Planctomycetes with Order-, Genus-, and Strain-Specific 16S rRNA-Targeted Probes

  • D. Gade
  • H. Schlesner
  • F. O. Glöckner
  • R. Amann
  • S. Pfeiffer
  • M. Thomm
Article

Abstract

A specific 16S rRNA-targeted oligonucleotide probe (PIR1223) for the genus Pirellula and a species-specific probe (RB454) for Pirellula sp. strain SH1 have been designed and optimized. Together with the already existing order-specific probe PLA886, the two newly designed probes were used to detect and identify planctomycetes, pirellulae, and close relatives of Pirellula sp. strain SH1 in different habitats. With the help of these probes for detection and identification, bacteria of the genus Pirellula were detected and cultivated from tissue of the Mediterranean sponge Aplysina aerophoba and from the water column of the Kiel Fjord. An unexpected result was the close phylogenetic relationship of the isolate from the sponge and the brackish water habitat Kiel Fjord as revealed by DNA/DNA hybridization.

Keywords

Sponge Anammox Sponge Tissue Compost Plant Kiel Fjord 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

We gratefully acknowledge U. Hentschel and A.B. Friedrich from the Institut für Molekulare Infektionsbiologie, Universität Würzburg, for providing the sponge material and for the preparation and fixation of the sponge material for FISH studies.

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Copyright information

© Springer-Verlag 2004

Authors and Affiliations

  • D. Gade
    • 1
    • 2
  • H. Schlesner
    • 2
  • F. O. Glöckner
    • 1
  • R. Amann
    • 1
  • S. Pfeiffer
    • 3
  • M. Thomm
    • 2
  1. 1.Max-Planck-Institut für Marine Mikrobiologie, Celsiusstr. 1, D-28359 BremenGermany
  2. 2.Institut für Allgemeine MikrobiologieUniversität Kiel, Am Botanischen Garten 1-9, D-24118 KielGermany
  3. 3.Zentrale MikroskopieBiologiezentrum der Universität Kiel, Am Botanischen Garten 5, D-24098 KielGermany

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