Archaeal nucleic acids in picoplankton from great lakes on three continents
- 119 Downloads
Phylogenetic analysis of PCR-amplified 16S rRNA genes revealed the presence of archaea in picoplankton collected from the Laurentian Great Lakes in North America, Africa’s Lake Victoria, and Lakes Ladoga and Onega in northeastern Eurasia. From 1 to 10% of the rRNA extracted from size-fractionated picoplankton (>0.2 µm but <1.2 µm) collected in the epilimnion and hypolimnion of these lakes was specific to the Archaea, whereas the majority of rRNA was derived from Bacteria. Analysis of the 16S rRNA genes cloned from these samples indicated they were closely related to crenarchaeal sequences that have been widely characterized from marine environments. The presence of nearly identical 16S rDNA clones in several of these geographically disparate lakes suggests a cosmopolitan distribution of specific subgroups of these Archaea in freshwater environments. Despite their abundance in the water column of freshwater lakes, we have no representatives of these crenarchaea in pure culture, and so their physiological characteristics and ecological role remain unknown.
KeywordsArchaea Great Lake Nucleic Acid Extract Laurentian Great Lake North American Great Lake
Unable to display preview. Download preview PDF.
- 1.Aim EW, Oerther DB, Larsen N, Stahl DA, Raskin L (1996) The oligonucleotide probe database. Appl Environ Microbiol 62:3557–3559Google Scholar
- 13.Fuhrman JA, Comeau DE, Hagstorm A, Chan AM (1998) Extraction from natural planktonic microorganisms of DNA suitable for molecular biological studies. Appl Environ Microbiol 54:1426–1429Google Scholar
- 20.Hicks RE, Pascoe DA (2001) A comparison of cyanobacterial dominance within the picoplankton of the North American Great Lakes estimated by 16S rRNA-based hybridizations and direct cell counts. In: Munawar M, Hecky RE (Eds.) The Great Lakes of the World (GLOW): Food-Web, Health, and Integrity. Backhuys Publishers, Leiden, The Netherlands, pp 363–374Google Scholar
- 42.Stahl DA, Amann R (1991) Development and application of nucleic acid probes in bacterial systematics. In: Stackebrandt E, Goodfellow M (Eds.) Nucleic Acid Techniques in Bacterial Systematics. John Wiley & Sons, Chichester, UK, pp 205–248Google Scholar
- 48.Verschuren D, Edgington DN, Kling HJ, Johnson TC (1998) Silica depletion in Lake Victoria: Sedimentary signals at offshore stations. J Gt Lakes Res 24:118–130Google Scholar