Microbial Ecology

, Volume 49, Issue 2, pp 209–217 | Cite as

Methanogen Communities in a Drained Bog: Effect of Ash Fertilization

  • P.E. GalandEmail author
  • H. Juottonen
  • H. Fritze
  • K. Yrjälä


Forestry practises such has drainage have been shown to decrease emissions of the greenhouse gas methane (CH4) from peatlands. The aim of the study was to examine the methanogen populations in a drained bog in northern Finland, and to assess the possible effect of ash fertilization on potential methane production and methanogen communities. Peat samples were collected from control and ash fertilized (15,000 kg/ha) plots 5 years after ash application, and potential CH4 production was measured. The methanogen community structure was studied by DNA isolation, PCR amplification of the methyl coenzyme-M reductase (mcr) gene, denaturing gradient gel electrophoresis (DGGE), and restriction fragment length polymorphism (RFLP) analysis. The drained peatland showed low potential methane production and methanogen diversity in both control and ash-fertilized plots. Samples from both upper and deeper layers of peat were dominated by three groups of sequences related to Rice cluster-I hydrogenotroph methanogens. Even though pH was marginally greater in the ash-treated site, the occurrence of those sequences was not affected by ash fertilization. Interestingly, a less common group of sequences, related to the Fen cluster, were found only in the fertilized plots. The study confirmed the depth related change of methanogen populations in peatland.


Clone Library Restriction Fragment Length Polymorphism Analysis Restriction Fragment Length Polymorphism Pattern Hydrogenotroph Methanogen Methanogen Community 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



We thank Mikko Moilanen and Jorma Issakainen for taking samples. The work was funded by the Finnish Academy.


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Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  • P.E. Galand
    • 1
    Email author
  • H. Juottonen
    • 1
  • H. Fritze
    • 2
  • K. Yrjälä
    • 1
  1. 1.Department of Biological and Environmental Sciences, Division of General MicrobiologyUniversity of HelsinkiHelsinkiFinland
  2. 2.Finnish Forest Research InstituteVantaa Research CentreVantaaFinland

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