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Microbial Ecology

, Volume 46, Issue 2, pp 145–160 | Cite as

Microbial community responses to atrazine exposure and nutrient availability: Linking degradation capacity to community structure

  • E. D. Rhine
  • J. J. Fuhrmann
  • M. Radosevich
Article

Abstract

Repeated pesticide exposure may enhance biodegradation through selective enrichment of pesticide-metabolizing microorganisms, particularly when the compound is used as a C and energy source. The relationship between pesticide application history and degradation rate is unclear when the chemical is utilized as a nutrient source other than C. Atrazine, a poor source of C and energy, was chosen as a model compound because it can serve as an N source for some microorganisms. Soils with (H-soil) and without (NH-soil) prior s-triazine treatment history were repeatedly exposed to atrazine and a variety of C and N source amendments. Exposure to atrazine and inorganic-N availability were the dominant factors leading to the development of microbial communities with an enhanced capacity to degrade atrazine. The density of the atrazine-degrading microorganisms increased immediately, up to 1000-fold, with atrazine exposure in the H-soil, but comparable increases were not observed in the NH-soil until 12 weeks following laboratory acclimation, despite high rates of atrazine mineralization in these soils immediately following the acclimation period. Whole-soil fatty acid methyl ester (FAME) analysis showed that the application of alternative C and N sources in addition to atrazine resulted in a microbial community composition that was distinctly different from that in either the atrazine-alone treatment or water controls for both the H- and NH-soils. These data suggest that the microbial communities in both soils were altered differently in response to the treatments but developed a similar enhanced capacity to mineralize atrazine.

Keywords

Pectin Fatq Acid Methyl Ester Atrazine Much Probable Number Cyanuric Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Abdelhafid R, Houot S, Barriuso E (2000) Dependence of atrazine degradation on C and N availability in adapted and non-adapted soils. Soil Biol Biochem 32:389–401CrossRefGoogle Scholar
  2. 2.
    Alvey S, Crowley DE (1995) Influence of organic amendments on biodegradation of atrazine as a nitrogen-source. J Environ Qual 24:1156–1162CrossRefGoogle Scholar
  3. 3.
    Barriuso E, Houot S (1996) Rapid mineralization of the s-triazine ring of atrazine in soils in relation to soil management. Soil Biol Biochem 28:1341–1348CrossRefGoogle Scholar
  4. 4.
    Bichat F, Sims GK, Mulvaney RL (1999) Microbial utilization of heterocyclic nitrogen from atrazine. Soil Sei Soc Am J 63:100–110CrossRefGoogle Scholar
  5. 5.
    Behki RM, Khan SU (1986) Degradation of atrazine by Pseudomcmas-N-dealkylation and dehalogenation of atrazine and its metabolites. J Agric Food Chem 34:746–749CrossRefGoogle Scholar
  6. 6.
    Buhler DD, Randall GW, Koskinen WC, Wyse DL (1993) Water-quality-atrazine and alachlor losses from subsurface tile drainage of a clay loam soil. J Environ Qual 22:583–588CrossRefGoogle Scholar
  7. 7.
    Cook AM (1987) Biodegradation of s-triazine xenobiotics. FEMS Microbiol Rev 46:93–116CrossRefGoogle Scholar
  8. 8.
    Cook AM, Hütter R (1981) s-Triazines as nitrogen-sources for bacteria. J Agric Food Chem 29:1135–1143CrossRefGoogle Scholar
  9. 9.
    Entry JA, Mattson KG, Emmingham WH (1993) The influence of nitrogen on atrazine and 2,4-dichlorophenoxyacetic acid mineralization in grass lands. Biol Fertil Soil 16:179–182CrossRefGoogle Scholar
  10. 10.
    Entry JA, Emmingham WH (1995) The influence of dairy manure on atrazine and 2,4-dichlorophenoxyacetic acid mineralization in pasture soils. Can J Soil Sei 75:379–383Google Scholar
  11. 11.
    Franzluebbers AJ, Nazih N, Stuedemann JA, Fuhrmann JJ, Schomberg HH, Hartel PG (1999) Soil carbon and nitrogen pools under low- and high-endophyte-infected tall fescue. Soil Sei Soc Am J 63:1687–1694Google Scholar
  12. 12.
    Gebendinger N, Radosevich M (1999) Inhibition of atrazine degradation by cyanazine and exogenous nitrogen in bacterial isolate M91-3. Appl Microbiol Biotechnol 51:375–381PubMedCrossRefGoogle Scholar
  13. 13.
    Hance RJ (1973) The effect of nutrients on the decomposition of soil herbicides atrazine and linuron incubation with soil. Pestic Sei 4:817–822CrossRefGoogle Scholar
  14. 14.
    Harman-Fetcho JA, McConnell LL, Baker JE (1999) Agricultural pesticides in the Patuxent River, a tributary of the Chesapeake Bay. J Environ Qual 28:928–938Google Scholar
  15. 15.
    Hayes TB, Collins, A, Lee M, Mendoza M, Noriega N, Stuart AA, Vonk A (2002) Hermaphroditic, demasculinized frogs after exposure to the herbicide atrazine at low ecologically relevant doses. Proc Natl Acad Sei USA 99:5476–5480CrossRefGoogle Scholar
  16. 16.
    Hoffman RS, Capel PD, Larson SJ (2000) Comparison of pesticides in eight US urban streams. Environ Toxicol Chem 19:2249–2258CrossRefGoogle Scholar
  17. 17.
    Horswell J, Hodge A, Killham K (1997) Influence of plant carbon on the mineralization of atrazine residues in soils. Chemospere 34:1739–1751CrossRefGoogle Scholar
  18. 18.
    Jayachandran K, Stolpe NB, Moorman TB, Shea PJ (1998) Application of C-14-most-probable-number technique to enumerate atrazine-degrading microorganisms in soil. Soil Biol Biochem 30:523–529CrossRefGoogle Scholar
  19. 19.
    Ka JO, Holben WE, Tiedje JM (1994) Genetic and phenotypic diversity of 2,4-dichlorophenoxyacetic acid (2,4-D)-degrading bacteria isolated from 2,4-D-treated field soils. Appl Environ Microbiol 60:1106–1115PubMedGoogle Scholar
  20. 20.
    Kolpin DW, Barbash JE, Gilliom RJ (2000) Pesticides in ground water of the United States, 1992–1996. Ground Wat 38:858–863CrossRefGoogle Scholar
  21. 21.
    Mandelbaum RT, Wackett LP, Allan DL (1993) Mineralization of the s-triazine ring of atrazine by stable bacterial mixed cultures. Appl Environ Microbiol 59:1695–1701PubMedGoogle Scholar
  22. 22.
    Mandelbaum RT, Allan DL, Wachett LP (1995) Isolation and characterization of a Pseudomonas sp. that mineralizes the s-triazine herbicide atrazine. Appl Environ Microbiol 61:1451–1457PubMedGoogle Scholar
  23. 23.
    Ostrofsky EB, Traina SJ, Tuovinen OH (1997) Variation in atrazine mineralization rates in relation to agricultural management practice. J Environ Qual 26:647–657Google Scholar
  24. 24.
    Radosevich M, Traina S J, Tuovinen OH (1996) Biodegradation of atrazine in surface soils and subsurface sediments collected from an agricultural research farm. Biodegradation 7:137–149PubMedCrossRefGoogle Scholar
  25. 25.
    Radosevich M, Traina SJ, Tuovinen OH (1997) Atrazine mineralization in laboratory-aged soil microcosms inoculated with s-triazine-degrading bacteria. J Environ Qual 26:206–214Google Scholar
  26. 26.
    Radosevich M, Traina SJ, Hao YL, Tuovinen OH (1995) Degradation and mineralization of atrazine by a soil bacterial isolate. Appl Environ Microbiol 61:297–302PubMedGoogle Scholar
  27. 27.
    Smith GA, Nickels JS, Kerger BD, Davis JD, Collins SP, Wilson JT, McNabb JF, White DC (1986) Quantitative characterization of microbial biomass and community structure in subsurface material—a prokaryotic consortium responsive to organic contamination. Can J Microbiol 32:104–111Google Scholar
  28. 28.
    Solomon KR, Baker DB, Richards RP, Dixon DR, Klaine SJ, LaPoint TW, Kendall RJ, Weisskopf CP, Giddings JM, Giesy JP, Hall LW, Williams WM (1996) Ecological risk assessment of atrazine in North American surface waters. Environ Toxicol Chem 15:31–74CrossRefGoogle Scholar
  29. 29.
    Struthers JK, Jayachandran K, Moorman TB (1998) Bio-degradation of atrazine by Agrobacterium radiobacter J14a and use of this strain in bioremediation of contaminated soil. Appl Environ Microbiol 64:3368–3375PubMedGoogle Scholar
  30. 30.
    Topp E, Tessier L, Gregorich EG (1996) Dairy manure incorporation stimulates rapid atrazine mineralization in an agricultural soil. Can J Soil Sei 76:403–409Google Scholar
  31. 31.
    Woomer PL (1994) Most probable number counts. In: Weaver RW, Angle JS, Bottomly P, Bezdicek D, Smith S, Tabatabai A, Wollum A (Eds.) Methods of Soil Analysis, Part 2. Microbiological and Biochemical Properties. SSSA Book Series, Madison, WI, pp 59–79Google Scholar
  32. 32.
    Yanze-Kontchou C, Gschwind N (1994) Mineralization of the herbicide atrazine as a carbon source by a Pseudomonas strain. Appl Environ Microbiol 60:4297–4302PubMedGoogle Scholar
  33. 33.
    Yassir A, Lagacherie B, Houot S, Soulas G (1999) Microbial aspects of atrazine biodegradation in relation to history of soil treatment. Pestic Sei 55:799–809CrossRefGoogle Scholar
  34. 34.
    Zelles L (1999) Fatty acid patterns of phospholipids and lipopolysaccharides in the characterization of microbial communities in soil: a review. Biol Fertil Soils 29:111–129CrossRefGoogle Scholar

Copyright information

© Springer-Verlag New York Inc 2003

Authors and Affiliations

  • E. D. Rhine
    • 1
    • 2
  • J. J. Fuhrmann
    • 1
  • M. Radosevich
    • 1
    • 3
  1. 1.Delaware Agricultural Experiment Station, Department of Plant and Soil Sciences, College of Agricultural and Natural ResourcesUniversity of DelawareNewarkUSA
  2. 2.Agricultural and Environmental Biotechnology CenterRutgers UniversityNew BrunswickUSA
  3. 3.Biosystems Engineering and Environmental ScienceUniversity of TennesseeKnoxvilleUSA

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