Response to Comment on: “Estimating the Bioconcentration Factors of Hydrophobic Organic Chemicals from Biotransformation Rates Using Rainbow Trout Hepatocytes”
We thank Dr. Goss for his interest in our paper (Trowell et al. 2018) and his attention to detail regarding the methods used in the extrapolation of in vitro biotransformation rates of chemicals measured in trout hepatocytes to in vivo biotransformation rates in the liver and whole body of fish. Considering the broad interest in in vitro-to-in vivo extrapolation (IVIVE) methods for accelerating bioaccumulation testing of commercial chemicals and minimizing animal use, his request for further clarification is worthy of discussion. Hence, we welcome the comment from Dr. Goss and hope that this discussion will help to improve the IVIVE of biotransformation rates for the purpose of conducting bioaccumulation assessments.
Dr. Goss’ main points of interest are (1) the difference between Nichols et al. (2013) and the Trowell et al. (2018) and Lee et al. (2017) IVIVE approaches; and (2) the appearance of a dimensionless ratio of mLincubation/mLliver in the extrapolation.
Nichols et al. (2013)...
- Nichols JW, Huggett DB, Arnot JA, Fitzsimmons PN, Cowan-Ellsberry CE (2013) Towards improved models for predicting bioconcentration of well-metabolized chemicals by rainbow trout using measured rates of in vitro intrinsic clearance. Environ Toxicol Chem 32:1611–1612Google Scholar