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Response to Comment on: “Estimating the Bioconcentration Factors of Hydrophobic Organic Chemicals from Biotransformation Rates Using Rainbow Trout Hepatocytes”

  • Frank A. P. C. GobasEmail author
  • Yung-Shan Lee
  • Leslie J. Saunders
  • Margo M. Moore
  • Jennifer J. Trowell
  • Christopher J. Kennedy
Reply to Letter to the Editor

We thank Dr. Goss for his interest in our paper (Trowell et al. 2018) and his attention to detail regarding the methods used in the extrapolation of in vitro biotransformation rates of chemicals measured in trout hepatocytes to in vivo biotransformation rates in the liver and whole body of fish. Considering the broad interest in in vitro-to-in vivo extrapolation (IVIVE) methods for accelerating bioaccumulation testing of commercial chemicals and minimizing animal use, his request for further clarification is worthy of discussion. Hence, we welcome the comment from Dr. Goss and hope that this discussion will help to improve the IVIVE of biotransformation rates for the purpose of conducting bioaccumulation assessments.

Dr. Goss’ main points of interest are (1) the difference between Nichols et al. (2013) and the Trowell et al. (2018) and Lee et al. (2017) IVIVE approaches; and (2) the appearance of a dimensionless ratio of mLincubation/mLliver in the extrapolation.

Nichols et al. (2013)...

References

  1. Lee Y-S, Lo JC, Otton SV, Moore MM, Kennedy CJ, Gobas FAPC (2017) In vitro to in vivo extrapolation of biotransformation rates for assessing bioaccumulation of hydrophobic organic chemicals in mammals. Environ Toxicol Chem.  https://doi.org/10.1002/etc.3718 Google Scholar
  2. Nichols JW, Huggett DB, Arnot JA, Fitzsimmons PN, Cowan-Ellsberry CE (2013) Towards improved models for predicting bioconcentration of well-metabolized chemicals by rainbow trout using measured rates of in vitro intrinsic clearance. Environ Toxicol Chem 32:1611–1612Google Scholar
  3. Trowell JJ, Gobas FAPC, Moore MM, Kennedy CJ (2018) Estimating the bioconcentration factors of hydrophobic organic chemicals from biotransformation rates using rainbow trout hepatocytes. Arch Environ Contam Toxicol.  https://doi.org/10.1007/s00244-018-0508-z Google Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Frank A. P. C. Gobas
    • 1
    Email author
  • Yung-Shan Lee
    • 1
  • Leslie J. Saunders
    • 1
  • Margo M. Moore
    • 1
  • Jennifer J. Trowell
    • 1
  • Christopher J. Kennedy
    • 1
  1. 1.Simon Fraser UniversityBurnabyCanada

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