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Swimming, Swarming, Twitching, and Chemotactic Responses of Cupriavidus metallidurans CH34 and Pseudomonas putida mt2 in the Presence of Cadmium

  • Saba Shamim
  • Abdul Rehman
  • Mahmood Hussain Qazi
Article

Abstract

To use of microorganisms for bioremediation purposes, the study of their motility behavior toward metals is essential. In the present study, Cupriavidus metallidurans CH34 and Pseudomonas putida mt2 were used as cadmium (Cd)-resistant and -sensitive bacteria, respectively, to evaluate the effects of Cd on their motility behaviors. Potassium morpholinopropane sulfonate (MOPS) buffer was used to observe the motility behavior of both isolates. Movement of mt2 was less in MOPS buffer compared with CH34, likely reflecting the mono-flagellated nature of mt2 and the peritrichous nature of CH34. The swimming, swarming, twitching, and chemotaxis behaviors of mt2 were greater in the presence of glucose than that of Cd. mt2 exhibited negative motility behaviors when exposed to Cd, but the opposite effect was seen in CH34. Cd was found to be a chemorepellent for mt2 but a chemoattractant for CH34, suggesting that CH34 is a potential candidate for metal (Cd) bioremediation.

Keywords

Motility Behavior Chemotactic Response Bacterial Chemotaxis Sensory Adaptation Flagellar Motor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

The authors thank Deutscher Akademischer Austauschdienst for funding this work. We also thank Dr. Lukas Y. Wick for providing laboratory facilities at Department of Environmental Microbiology-UMB, Helmholtz Centre for Environmental Research-UFZ for conducting the chemotaxis experiments. The technical assistance provided by Jana Reichenbach is also gratefully acknowledged.

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Saba Shamim
    • 1
    • 2
    • 3
  • Abdul Rehman
    • 1
  • Mahmood Hussain Qazi
    • 3
  1. 1.Department of Microbiology and Molecular GeneticsUniversity of the PunjabLahorePakistan
  2. 2.Department of Environmental Microbiology-UMBHelmholtz Centre for Environmental Research-UFZLeipzigGermany
  3. 3.Department of Microbiology, Institute of Molecular Biology and Biotechnology (IMBB)The University of LahoreLahorePakistan

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