Toxicity of Polyfluorinated and Perfluorinated Compounds to Lettuce (Lactuca sativa) and Green Algae (Pseudokirchneriella subcapitata)
Recently, polyfluorinated and perfluorinated compounds (PFCs) have been detected in most surface waters around the world. Because some PFCs are persistent and tend to accumulate in surface waters, their potential adverse effects to aquatic organisms have received increasing attention. Nevertheless, currently available toxicity information is limited. The aim of this study was to evaluate the toxicity effects of seven PFCs on root elongation of lettuce (Lactuca sativa) and photosynthesis of green algae (Pseudokirchneriella subcapitata). It was found that the toxicity profiles of both species tested were similar and had good relations with the fluorinated carbon-chain length of the PFCs investigated. One of the compounds tested, perfluorobutanoic acid, was found to be more toxic than expected in the algae test, which may be related with acidification of the test solution. It was concluded that because short-chained PFCs are becoming the predominant PFC pollutants in surface waters, their long-term toxicity and mixture toxicity with other PFCs should be studied in greater detail.
KeywordsRoot Elongation QSAR Model Mixture Toxicity Scenedesmus Obliquus Lettuce Seed
This work was funded by the FP7 project CADASTER (Grant No. FP7-ENV-2007-1-212668) and the fundamental research funds for the central universities of China (Grant No. DLMU 2011QN053).
- Kissa E (2001) Fluorinated surfactants and repellants, 2nd edn. Marcel Decker, New York, NYGoogle Scholar
- Organisation for Economic Co-operation and Development (2002) Hazard assessment of perfluorooctane sulfonate (PFOS) and its salts. ENV/JM/RD(2002)17/FINAL. Joint Meeting of the Chemicals Committee and the Working Party on Chemicals, Pesticides, and Biotechnology, Environment Directorate, Organisation for Economic Co-operation and Development, Paris, FranceGoogle Scholar
- Organisation for Economic Co-operation and Development (2006) OECD guidelines for the testing of chemicals / section 2: effects on biotic systems test no. 208: Terrestrial plant test: Seedling emergence and seedling growth testGoogle Scholar
- Schreiber U, Bilger W, Neubauer C (1994) Chlorophyll fluorescence as a nonintrusive indicator for rapid assessment of in vivo photosynthesis. In: Schulze ED, Caldwell MM (eds) Ecophysiology of photosynthesis. Springer, Berlin, pp 49–69Google Scholar
- Taniyasu S, Kannan K, Yeung LW, Kwok KY, Lam PKS, Yamashita N (2008) Analysis of trifluoroacetic acid and other short-chain perfluorinated acids (C2–C4) in precipitation by liquid chromatography-tandem mass spectrometry: comparison to patterns of long-chain perfluorinated acids (C5-C18). Anal Chim Acta 619:221–230CrossRefGoogle Scholar
- United States Environmental Protection Agency (1996) Ecological effects test guidelines (OPPTS 850.4200): seed germination/root elongation toxicity test. EPA 712-C-96-154. Washington, DCGoogle Scholar
- Van Beusekom SAM, Admiraal W, Sterkenburg A, De Zwart D (1999) ECO notitie 98/09. Handleiding PAM-Test (in dutch). National Institute for Public Health and the Environment, Bilthoven, The NetherlandsGoogle Scholar
- Verweij W, Durand, AM, Maas JL, Van der Grinten E (2009) PAM test: acute effects on photosynthesis in algae. In protocols belonging to the report “Toxicity measurements in concentrated water samples.” National Institute for Public Health and the Environment report 607013011/2009, pp 41–51Google Scholar