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Modulation of Thyroid Hormone Concentrations in Serum of Rats Coadministered with Perchlorate and Iodide-Deficient Diet

  • Tatsuya Kunisue
  • Jeffrey W. Fisher
  • Kurunthachalam KannanEmail author
Article

Abstract

Perchlorate can perturb thyroid hormone (TH) homeostasis by competitive inhibition of iodide uptake by the thyroid gland. Until recently, the effects of perchlorate on TH homeostasis were examined by measuring serum concentrations of THs by immunoassay (IA) methods. IA methods are sensitive, but for TH analysis they are compromised by lack of adequate specificity. In this study, we determined the concentrations of six THs: l-thyroxine (T4), 3,3′,5-triiodo-l-thyronine (T3), 3,3′,5′-triiodo-l-thyronine (rT3), 3,5-diiodo-l-thyronine, 3,3′-diiodo-l-thyronine, and 3-iodo-l-thyronine in the serum of rats administered perchlorate by isotope (13C6-T4)-dilution liquid chromatography–tandem mass spectrometry. The method recoveries for THs spiked into a serum matrix were between 97.0% and 115%, with a coefficient of variation of 2.1% to 9.4%. Rats were placed on an iodide-deficient or iodide-sufficient diet for 2.5 months, and for the last 2 weeks of that period they were provided drinking water either without or with perchlorate (10 mg/kg body weight/day). No significant differences in serum concentrations of T3 and T4 were observed between rats given iodide-deficient and iodide-sufficient diets for 2 or 2.5 months. After 24 h of perchlorate exposure, significantly lower concentrations of T3 and T4 were found in the serum of rats administered the iodide-deficient diet but not in rats administered the iodide-sufficient diet. However, after 2 weeks of perchlorate exposure, TH levels in rats fed the iodide-sufficient diet were also significantly lower than those in control rats. Our results suggest that perchlorate affects TH homeostasis and that such effects are more pronounced under iodide-deficient nutrition.

Keywords

Thyroid Hormone Perchlorate Thyroid Gland Flow Injection Analysis System Provide Drinking Water 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This study was supported by USEPA STAR Cooperative Agreement R832134 and by a biomonitoring grant (Grant No. 1U38EH000464-01) from the Centers for Disease Control and Prevention (CDC), Atlanta, GA, United States. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of the CDC.

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Tatsuya Kunisue
    • 1
  • Jeffrey W. Fisher
    • 3
  • Kurunthachalam Kannan
    • 1
    • 2
    • 4
    Email author
  1. 1.Wadsworth CenterNew York State Department of HealthAlbanyUSA
  2. 2.Department of Environmental Health SciencesSchool of Public Health, State University of New York at AlbanyAlbanyUSA
  3. 3.Department of Environmental Health ScienceCollege of Public Health, University of GeorgiaAthensUSA
  4. 4.State Key Laboratory of Urban Water Resources and EnvironmentIJRC PTS, Harbin Institute of TechnologyHarbinChina

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