Construction of River Model Biofilm for Assessing Pesticide Effects

  • Shohei Hayashi
  • Ji Eun Jang
  • Kazuhito Itoh
  • Kousuke Suyama
  • Hiroki Yamamoto


Due to the high importance of biofilms on river ecosystems, assessment of pesticides’ adverse effects is necessary but is impaired by high variability and poor reproducibility of both natural biofilms and those developed in the laboratory. We constructed a model biofilm to evaluate the effects of pesticides, consisting in cultured microbial strains, Pedobacter sp. 7-11, Aquaspirillum sp. T-5, Stenotrophomonas sp. 3-7, Achnanthes minutissima N71, Nitzschia palea N489, and/or Cyclotella meneghiniana N803. Microbial cell numbers, esterase activity, chlorophyll-a content, and the community structure of the model biofilm were examined and found to be useful as biological factors for evaluating the pesticide effects. The model biofilm was formed through the cooperative interaction of bacteria and diatoms, and a preliminary experiment using the herbicide atrazine, which inhibits diatom growth, indicated that the adverse effect on diatoms inhibited indirectly the bacterial growth and activity and, thus, the formation of the model biofilm. Toxicological tests using model biofilms could be useful for evaluating the pesticide effects and complementary to studies on actual river biofilms.


Atrazine Microbial Community Structure Esterase Activity Diuron Bacterial Number 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Shohei Hayashi
    • 1
  • Ji Eun Jang
    • 1
  • Kazuhito Itoh
    • 1
  • Kousuke Suyama
    • 1
  • Hiroki Yamamoto
    • 1
  1. 1.Faculty of Life and Environmental ScienceShimane UniversityMatsueJapan

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