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Assessment of Pyrene Bioavailability in Soil by Mild Hydroxypropyl-β-Cyclodextrin Extraction

  • Muhammad Imran Khan
  • Sardar Alam Cheema
  • Chaofeng ShenEmail author
  • Congkai Zhang
  • Xianjin Tang
  • Zaffar Malik
  • Xincai Chen
  • Yingxu Chen
Article

Abstract

Bioavailability of organic pollutants in soil is currently a much-debated issue in risk assessment of contaminated sites. Ecorisk of an organic pollutant in soil is strongly influenced by the properties of the soil and its contamination history. To evaluate the effect of aging on the availability of pyrene, earthworm (Eisenia fetida) accumulation and chemical extraction by exhaustive and nonexhaustive techniques in soil spiked with a range of pyrene levels (1.07, 9.72, 88.4, 152, and 429 μg g−1 dry soil) were measured in this study using both unaged (i.e., 0 days) and aged (i.e., 69, 150, and 222 days) soil samples. The results showed that the amount of pyrene accumulated by earthworms did not change greatly with aging time under different high-dose contamination levels, but changed significantly at lower concentrations. Moreover, aging (after 222 days) significantly decreased biological and chemical availability of pyrene. Furthermore, the relationship between earthworm bioaccumulation, hydroxypropyl-β-cyclodextrin (HPCD), and organic solvent extraction was investigated in order to find a suitable and rapid method to predict pyrene bioavailability. Results showed that, at different levels of pyrene, the mean values of earthworm uptake and HPCD extractability were 10–40% and 10–65%, respectively. Correlation (r 2 = 0.985) and extraction results for pyrene suggested that mild HPCD extraction was a better method to predict bioavailability of pyrene in soil compared with organic solvent extraction.

Keywords

PAHs Pyrene Aging Time Soxhlet Extraction Organic Solvent Extraction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work was supported by Science and Technology Foundation of Zhejiang Province (2008C23087) and Natural Science Foundation of Zhejiang Province (Z506039).

References

  1. Alexander M (1999) Biodegradation and bioremediation. Academic, New YorkGoogle Scholar
  2. Allan IJ, Semple KT, Hare R, Reid BJ (2006) Prediction of mono- and polycyclic aromatic hydrocarbon degradation in spiked soils using cyclodextrin extraction. Environ Pollut 144:562–571CrossRefGoogle Scholar
  3. Amellal S, Boivin A, Ganier PC, Schiavon M (2006) High sorption of phenanthrene in agricultural soils. Agron Sustain Dev 26:99–106CrossRefGoogle Scholar
  4. Barriuso E, Benoit P, Dubus IG (2008) Formation of pesticide nonextractable (bound) residues in soil: magnitude, controlling factors and reversibility. Environ Sci Technol 42:1845–1854CrossRefGoogle Scholar
  5. Barthe M, Pelletier E (2007) Comparing bulk extraction methods for chemically available polycyclic aromatic hydrocarbons with bioaccumulation in worms. Environ Chem 4:271–283CrossRefGoogle Scholar
  6. Bergknut M, Sehlin E, Lundstedt S, Andersson PL, Haglund P, Tysklind M (2007) Comparison of techniques for estimating PAH bioavailability: uptake in Eisenia foetida, passive samplers and leaching using various solvents and additives. Environ Pollut 145:154–160CrossRefGoogle Scholar
  7. Blyshak LA, Rossi TM, Patonay G, Warner IM (1988) Cyclodextrin modified solvent extraction for polynuclear aromatic hydrocarbons. Anal Chem 60:2127–2131CrossRefGoogle Scholar
  8. Bosma TNP, Middeldorp PJM, Schraa G, Zehnder AJB (1997) Mass transfer limitation of biotransformation: quantifying bioavailability. Environ Sci Technol 3:248–252CrossRefGoogle Scholar
  9. Cheema SA, Khan MI, Shen CF, Tang XJ, Lei Chen, Zhang CK, Chen YX (2010) Degradation of phenanthrene and pyrene in spiked soils by single and combined plants cultivation. J Hazard Mater 17:384–389CrossRefGoogle Scholar
  10. Chung N, Alexander M (2002) Effect of soil properties on bioavailability and extractability of phenanthrene and atrazine sequestered in soil. Chemosphere 48:109–115CrossRefGoogle Scholar
  11. Cuypers C, Pancras T, Grotenhuis T, Rulkens W (2002) The estimation of PAH bioavailability in contaminated sediments using hydroxypropyl-beta-cyclodextrin and triton X-100 extraction techniques. Chemosphere 46:1235–1245CrossRefGoogle Scholar
  12. Doick KJ, Clasper PJ, Urmann K, Semple KT (2006) Further validation of the HPCD-technique for the evaluation of PAH microbial availability in soil. Environ Pollut 144:345–354CrossRefGoogle Scholar
  13. Frederick KA, Babish JG (1982) Evaluation of mutagenicity and other adverse effects of occupational exposure to sodium azide. Regul Toxicol Pharm 2:308–322CrossRefGoogle Scholar
  14. Gevao B, Mordaunt C, Semple KT, Piearce TG, Jones KC (2001) Bioavailability of nonextractable (bound) pesticide residues to earthworms. Environ Sci Technol 35:501–507CrossRefGoogle Scholar
  15. Gomez-Eyles JL, Collins CD, Hodson ME (2010) Relative proportions of polycyclic aromatic hydrocarbons differ between accumulation bioassays and chemical methods to predict bioavailability. Environ Pollut 158:278–284CrossRefGoogle Scholar
  16. Hartnik T, Jensen J, Hermens JLM (2008) Nonexhaustive beta-cyclodextrin extraction as a chemical tool to estimate bioavailability of hydrophobic pesticides for earthworms. Environ Sci Technol 42:8419–8425CrossRefGoogle Scholar
  17. Hatzinger PB, Alexander M (1995) Effect of aging of chemicals in soil on their biodegradability and extractability. Environ Sci Technol 29:537–545CrossRefGoogle Scholar
  18. Hickman ZA, Reid BJ (2005) Towards a more appropriate water based extraction for the assessment of organic contaminant availability. Environ Pollut 138:299–306CrossRefGoogle Scholar
  19. Hickman ZA, Swindell AL, Allan IJ, Rhodes AH, Hare R, Semple KT, Reid BJ (2008) Assessing biodegradation potential of PAHs in complex multi contaminant matrices. Environ Pollut 156:1041–1045CrossRefGoogle Scholar
  20. Hua GX, Killham K, Singleton I (2006) Potential application of synchronous fluorescence spectroscopy to determine benzo[a]pyrene in soil extracts. Environ Pollut 139:272–278CrossRefGoogle Scholar
  21. Hua G, Broderick J, Semple KT, Killham K, Singleton I (2007) Rapid quantification of polycyclic aromatic hydrocarbons in hydroxypropyl-β-cyclodextrin (HPCD) soil extracts by synchronous fluorescence spectroscopy (SFS). Environ Pollut 148:176–181CrossRefGoogle Scholar
  22. Jager T (1998) Mechanistic approach for estimating bioconcentration of organic chemicals in earthworms (Oligochaeta). Environ Toxicol Chem 17:2080–2090CrossRefGoogle Scholar
  23. Jager T, van der Wal L, Fleuren RHLJ, Barendregt A, Hermens JLM (2005) Bioaccumulation of organic chemicals in contaminated soils: evaluation of bioassays with earthworms. Environ Sci Technol 39:293–298CrossRefGoogle Scholar
  24. Jian Y, Wang L, Peter PF, Yu HT (2004) Photomutagenicity of 16 polycyclic aromatic hydrocarbons from the US EPA priority pollutant list. Mutat Res 557:99–108Google Scholar
  25. Kraaij R, Seinen W, Tolls J, Cornelissen G, Belfroid A (2002) Direct evidence of sequestration in sediments affecting the bioavailability of hydrophobic organic chemicals to benthic deposit-feeders. Environ Sci Technol 36:3525–3529CrossRefGoogle Scholar
  26. Lanno R, Wells J, Conder J, Bradham K, Basta N (2004) The bioavailability of chemicals in soil for earthworms. Ecotoxicol Environ Safe 57:39–47CrossRefGoogle Scholar
  27. Liste H-H, Alexander M (2002) Butanol extraction to predict bioavailability of PAHs in soil. Chemosphere 46:1011–1017CrossRefGoogle Scholar
  28. Northcott GL, Jones KC (2001) Partitioning, extractability, and formation of nonextractable PAH residues in soil. 1. Compound differences in aging and sequestration. Environ Sci Technol 35:1103–1110CrossRefGoogle Scholar
  29. Papadopoulos A, Paton GI, Reid BJ, Semple KT (2007) Prediction of PAH biodegradation in field contaminated soils using a cyclodextrin extraction technique. J Environ Monit 9:516–522CrossRefGoogle Scholar
  30. Parrish ZD, White JC, Isleyen M, Gent MPN, Berger WI, Eitzer BD, Kelsey JW, Mattina MI (2006) Accumulation of weathered polycyclic aromatic hydrocarbons (PAHs) by plant and earthworm species. Chemosphere 64:609–618CrossRefGoogle Scholar
  31. Puglisi E, Patterson CJ, Paton GI (2003) Non-exhaustive extraction techniques (NEETs) for bioavailability assessment of organic hydrophobic compounds in soils. Agronomie 23:755–756CrossRefGoogle Scholar
  32. Puglisi E, Cappa F, Fragoulis G, Trevisan M, Del Re AMA (2007) Bioavailability and degradation of phenanthrene in compost amended soils. Chemosphere 67:548–556CrossRefGoogle Scholar
  33. Reid BJ, Jones KC, Semple KT (2000a) Bioavailability of persistent organic contaminants in soils and sediments e a perspective on mechanisms, consequences and means of assessment. Environ Pollut 108:103–112CrossRefGoogle Scholar
  34. Reid BJ, Stokes JD, Jones KC, Semple KT (2000b) Nonexhaustive cyclodextrin-based extraction technique for the evaluation of PAH bioavailability. Environ Sci Technol 34:3174–3179CrossRefGoogle Scholar
  35. Reid BJ, Stokes JD, Jones KC, Semple KT (2004) Influence of hydroxypropyl-beta-cyclodextrin on the extraction and biodegradation of phenanthrene in soil. Environ Toxicol Chem 23:550–556CrossRefGoogle Scholar
  36. Sabate J, Vinas M, Solanas AM (2006) Bioavailability assessment and environmental fate of polycyclic aromatic hydrocarbons in biostimulated creosote-contaminated soil. Chemosphere 63:1648–1659CrossRefGoogle Scholar
  37. Semple KT, Morriss AWJ, Paton GI (2003) Bioavailability of hydrophobic organic contaminants in soils: fundamental concepts and techniques for analysis. Eur J Soil Sci 54:809–818CrossRefGoogle Scholar
  38. Stokes JD, Wilkinson A, Reid BJ, Jones KC, Semple KT (2005) Prediction of PAH biodegradation in contaminated soils using an aqueous hydroxypropyl-beta-cyclodextrin extraction technique. Environ Toxicol Chem 24:1325–1330CrossRefGoogle Scholar
  39. Sun HW, Li JG (2005) Availability of pyrene in unaged and aged soils to earthworm uptake, butanol extraction and SFE. Water Air Soil Pollut 166:353–365CrossRefGoogle Scholar
  40. Swindell AL, Reid BJ (2006) Comparison of selected non-exhaustive extraction techniques to assess PAH availability in dissimilar soils. Chemosphere 62:1126–1134CrossRefGoogle Scholar
  41. Tang J, Alexander M (1999) Mild extractability and bioavailability of polycyclic aromatic hydrocarbons in soil. Environ Toxicol Chem 18:2711–2714CrossRefGoogle Scholar
  42. Tang J, Robertson BK, Alexander M (1999) Chemical extraction methods to estimate bioavailability of DDT, DDE, and DDD in soil. Environ Sci Technol 33:4346–4351CrossRefGoogle Scholar
  43. Tang J, Liste HH, Alexander M (2002) Chemical assays of availability to earthworms of polycyclic aromatic hydrocarbons in soil. Chemosphere 48:35–42CrossRefGoogle Scholar
  44. Tang XJ, Shen CF, Cheema SA, Khan MI, Zhang CK, Chen YX (2010) Heavy metal and persistent organic compound contamination in soil from Wenling: an emerging e-waste recycling city in Taizhou area, China. J Hazard Mater 173:653–660CrossRefGoogle Scholar
  45. Wang Z, Chen JW, Yang P, Qiao XL, Tian FL (2007) Polycyclic aromatic hydrocarbons in Dalian soils: distribution and toxicity assessment. J Environ Monit 9:199–204CrossRefGoogle Scholar
  46. White JC, Hunter M, Nam K, Pignatello JJ, Alexander M (1999) Correlation between biological and physical availabilities of phenanthrene in soils and soil humin in aging experiments. Environ Toxicol Chem 18:1720–1727CrossRefGoogle Scholar
  47. Wong F, Bidleman TF (2010) Hydroxypropyl-b-cyclodextrin as non-exhaustive extractant for organochlorine pesticides and polychlorinated biphenyls in muck soil. Environ Pollut. doi: 10.1016/j.envpol.2010.01.016

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Muhammad Imran Khan
    • 1
    • 2
  • Sardar Alam Cheema
    • 1
    • 2
  • Chaofeng Shen
    • 1
    Email author
  • Congkai Zhang
    • 1
  • Xianjin Tang
    • 1
  • Zaffar Malik
    • 3
  • Xincai Chen
    • 1
  • Yingxu Chen
    • 1
  1. 1.Institute of Environmental Science and TechnologyZhejiang UniversityHangzhouPeople’s Republic of China
  2. 2.Department of AgronomyUniversity of AgricultureFaisalabadPakistan
  3. 3.MOE Key Lab of Environmental Remediation and Ecosystem Health, College of Environmental and Resource SciencesZhejiang UniversityHangzhouPeople’s Republic of China

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