Assessing Toxicity of the Insecticide Thiacloprid on Chironomus riparius (Insecta: Diptera) Using Multiple End Points

  • Miriam Langer-JaesrichEmail author
  • Heinz-R. Köhler
  • Almut Gerhardt


Since data documentation on neonicotinic toxicity to nontarget organisms should be enhanced, we investigate the effects of thiacloprid, a novel neonicotinoid insecticide, on the sediment-dwelling nontarget insect Chironomus riparius. Further, we wanted to validate the sensitivity of end points on different biological levels and obtain the greatest amount of information regarding the effects of this compound by using a battery of several end points such as larval mortality, behavior, body weight gain, emergence rate, time of development, gender ratio, Hsp70 stress protein level, and larval mouthpart deformities after exposure at a concentration range of 0.1 to 1000 μg/L thiacloprid. C. riparius was impacted starting at concentrations of 0.5 μg/L, a concentration that can be considered environmentally relevant. Larval mortality, behavior, emergence, and Hsp70 protein level were sensitive indicators for the toxic effect of thiacloprid, whereas gender ratio and mouthpart morphology were not affected. In our case life-cycle end points like survival rate (LC50: 1.57 μg/L) and emergence rate (EC50: 0.54 μg/L) proved to be more sensitive than tested physiological end points for the neurotoxic insecticide.


Body Weight Gain Larval Mortality Hsp70 Level OECD Guideline Thiacloprid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The authors are grateful to anonymous reviewers for valuable comments on the manuscript. The study was supported by EU Integrated Project NoMiracle (Novel Methods for Integrated Risk Assessment of Cumulative Stressors in Europe; contract no. 003956 under the EU theme “Global Changes and Ecosystems” topic “Development of Risk Assessment Methodologies,” coordinated by Hans Løkke at NERI, DK-8600 Sikeborg, Denmark, granted to Almut Gerhardt, LimCo International.


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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Miriam Langer-Jaesrich
    • 1
    Email author
  • Heinz-R. Köhler
    • 1
  • Almut Gerhardt
    • 2
  1. 1.Animal Physiological Ecology DepartmentUniversity of TübingenTübingenGermany
  2. 2.LimCo InternationalIbbenbürenGermany

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