Validation and Application of a Method for the Determination of Total Chromium in Rat Tissues by Inductively Coupled Plasma Mass Spectrometry

  • Keith E. Levine
  • Matthew D. Stout
  • Glenn T. Ross
  • Amal S. Essader
  • Frank X. Weber
  • Peter M. Grohse
  • Reshan A. Fernando
  • Lisa S. Milstein
  • Michelle J. Hooth
  • Bradley J. Collins
Article

DOI: 10.1007/s00244-009-9397-5

Cite this article as:
Levine, K.E., Stout, M.D., Ross, G.T. et al. Arch Environ Contam Toxicol (2010) 58: 883. doi:10.1007/s00244-009-9397-5

Abstract

The validation of a method for the determination of chromium (Cr) in F-344/N rat tissues by inductively coupled plasma-mass spectrometry is described. Samples were analyzed after a rapid, open-vessel microwave digestion procedure. Performance of the method was evaluated using kidney tissue across a concentration range of 0.50–5.00 μg Cr/g tissue. Data for method linearity, accuracy, precision, digest stability, and storage stability are presented along with limits of detection and quantitation data. Data from a method cross-validation for B6C3F1 mouse kidney tissue are also presented. After validation, the method was applied to analyze samples collected in support of two chronic toxicity and carcinogenesis studies conducted by the National Toxicology Program.

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Keith E. Levine
    • 1
  • Matthew D. Stout
    • 2
  • Glenn T. Ross
    • 1
  • Amal S. Essader
    • 1
  • Frank X. Weber
    • 1
  • Peter M. Grohse
    • 1
  • Reshan A. Fernando
    • 1
  • Lisa S. Milstein
    • 1
  • Michelle J. Hooth
    • 2
  • Bradley J. Collins
    • 2
  1. 1.RTI InternationalResearch Triangle ParkUSA
  2. 2.National Toxicology ProgramNational Institute of Environmental Health SciencesResearch Triangle ParkUSA

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