Bee Honey as an Environmental Bioindicator of Pesticides’ Occurrence in Six Agricultural Areas of Greece
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The pollution of six agricultural areas of Greece (north, central, south) by insecticides used in crop protection has been investigated utilizing, as a bioindicator, bee honey produced in those areas. Honey samples collected randomly from apiaries located in those areas were analyzed for pesticide residues with a multianalytical method, able to determine simultaneously up to 10 organophosphorous insecticides from the same honey extract. Findings concerning the acaricide coumaphos were also included, even though it is not used in crop protection. Coumaphos is used to control the mite Varroa destructor, an external parasite of the honeybee. The above areas are cultivated in large extent with citrus trees or cotton or sunflower crops, which are good forages for honeybees. The main pests of those crops are insects; hence, insecticides are used on a large scale for crop protection. The most contaminated samples originated from citrus groves; 16 out of 19 had pesticide residues: 4 samples had chlorfenvinphos (21.05%), 10 had chlorpyrifos (52.63%) and 2 had phorate (10.53%). Out of 17 samples from cotton fields, residues were found in 8, phorate in 6 (35.29%), chlorfenvinphos in 1 (5.88%), and chlorpyrifos in 1 (5.88%). Out of nine samples from fields of sunflower, four had phorate residues (44.44%). In brief, from the 50 analyzed samples, residues of chlorfenvinphos were detected in 5 samples (10%), residues of chlorpyrifos in 11 samples (22%), and residues of phorate in 12 samples (24%). Their levels ranged between 0.70 and 0.89 μg/kg. Coumaphos residues ranged from 0.10 up to 4.80 μg/kg and were derived exclusively from beehives treated with Perizin (the commercial formulation of coumaphos) for Varroa control.
This study indicates that in agricultural areas with developed apiculture, useful information about the occurrence and the distribution of pesticide residues due to crop protection treatments can be derived from the analysis of randomly collected honey samples, used as bioindicators. It also shows that, very often, the chemicals used by apiculturists inside the hives in order to control disease are the main pollutants of the produced honey.
KeywordsChlorpyrifos Malathion Crop Protection Honey Sample Phorate
- Albero B, Sanchez-Brunete C, Tadeo JL (2001) Multiresidue determination of pesticides in honey by matrix solid-phase dispersion and gas chromatography with electron-capture detection. J AOAC Int 84:1165–1171Google Scholar
- Alexanister ML, Bodnarchuk LI (1999) Beekeeping products as bioindicators of radioactive contamination in a certain zone. Apiacta 34:93–96Google Scholar
- Balayiannis DP, Krokos FD, Balayannis PG (2007) Levels and persistence of malathion, coumaphos and fluvalinate residues in Greek honey. J Sci Food Agric (in review)Google Scholar
- Bogdanov S, Kilchenmann V, Imdorf A (1998) Acaricide residues in some bee products. J Apicult Res 37:57–67Google Scholar
- Celli G, Porrini C, Radeghieri P, et al. (1996) Honeybees as bioindicators for the presence of pesticides in the agroecosystem. Field tests. Insect Social Life 1:207–212Google Scholar
- Devillers J, Pham- Délègue MH (eds) (2002) Honey bees: Estimating the environmental impact of chemicals. Taylor & Francis, LondonGoogle Scholar
- Garcia MA, Pena Cresente R, Herrero C, Fernandez MI (1993) Residues in honey of organophosphorous pesticides used for plant protection in the district of Lemos Lugo and Sierras Orientales. In: Dasio CT (ed) Congreso Galego de Proteccion Vexetal. Edicios do Castro, Coruna, p 234–241Google Scholar
- Haarmann TK (1997) Honey bees as indicators of radionuclide contamination: exploring colony variability and temporal contamination. J Apic Res 36:77–87Google Scholar
- Porrini C, Ghini S, Girotti S, Sabatini AG, Gattavecchia E, Celli G (2002) Use of honey bees as bioindicators of environmental pollution in Italy. In: Devillers J, Pham- Délègue MH (eds) Honey bees: Estimating the environmental impact of chemicals. Taylor & Francis, LondonGoogle Scholar
- Taccheo MB, De Paoli M, Mondir R, et al. (1993) Honey bee as indicator of agricultural pollution. In: Del Re AAM, Capri E, Evans SP, Natali P, Trevsian M (eds) Atti del IX Symposium Pesticide Chemistry: Degradation and Mobility of Xenobiotics, Piacenza, 11–13 Ottobre 1993. Edizioni Biagini (Lucca), p 32–45Google Scholar