Plasma Cholinesterase Levels and Health Symptoms in Peruvian Farm Workers Exposed to Organophosphate Pesticides

  • Hector C. Cataño
  • Elizabeth Carranza
  • Carlos Huamaní
  • Antonio F. Hernández
Article

Abstract

The purpose of this study was to examine plasma cholinesterase (PChE) changes and the adverse health effects associated with chronic low-dose exposure to organophosphates (OPs) in a Peruvian agricultural population. A cross-sectional study with a clinical interview and blood tests was performed among 213 farm workers from two subtropical valleys in Peru. The control group consisted of 78 nonexposed workers from the same areas. PChE levels from the two exposed subgroups (pesticide applicators and other agricultural jobs) were significantly lower than those of controls (1554 ± 315 U/l, 1532 ± 340 U/l, and 1787 ± 275 U/l, respectively). Fifteen percent of the exposed population reported a past poisoning by pesticides, all of them needing medical evaluation and treatment. They had significantly lower PChE levels as compared to those without this antecedent. Approximately 61% of the exposed workers reported pesticide-related symptoms, but no significant difference was found in their PChE as compared to workers without symptoms. On the other hand, the use of personal protective equipment (PPE) was significantly associated with higher PChE levels and with a lower risk of reporting pesticide-related symptoms, which supports the benefit from using appropriate protective measures. In conclusion, data indicate that farm workers exposed to OPs in developing countries need to be monitored by means of PChE and an examination of their clinical status, which would allow identification of farm workers most at risk from pesticide toxicity. The use of correct PPE is highly recommended.

Notes

Acknowledgments

We thank Percy Herrera and Carlos Cabrera for their technical help. This study was partially funded by Fundación Instituto Hipólito Unanue (Grant number 880/2004-CC-CD).

References

  1. Carlock LL, Chen WL, Gordon EB, Killeen JC, Manley A, Meyer LS, Mullin LS, Pendino KJ, Percy A, Sargent DE, Seaman LR, Svanborg NK, Stanton RH, Tellone CI, Van Goethem DL (1999) Regulating and assessing risks of cholinesterase-inhibiting pesticides: Divergent approaches and interpretations. J Toxicol Environ Health B 2:105–160CrossRefGoogle Scholar
  2. Clegg DJ, van Gemert M (1999) Expert panel report of human studies on chlorpyrifos and/or other organophosphate exposures. J Toxicol Environ Health B 2:257–279CrossRefGoogle Scholar
  3. Ciesielski S, Loomis DP, Mims SR, Auer A (1994) Pesticide exposures, cholinesterase depression, and symptoms among North Carolina migrant farmworkers. Am J Public Health 84:446–451Google Scholar
  4. Cole DC, Carpio F, Julian J, León N (1998) Assessment of peripheral nerve function in an Ecuadorian rural population exposed to pesticides. J Toxicol Environ Health A 55:77–91CrossRefGoogle Scholar
  5. Coye MJ, Lowe JA, Maddy KT (1986) Biological monitoring of agricultural workers exposed to pesticides: I. Cholinesterase activity determinations. J Occup Med 28:619–627CrossRefGoogle Scholar
  6. Cucuianu M, Nistor T, Hancu N, Orbai P, Muscurel C, Stoian I (2000) Serum cholinesterase activity correlates with serum insulin, C-peptide and free fatty acids levels in patients with type 2 diabetes. Rom J Intern Med 40:43–51Google Scholar
  7. Dyer SM, Cattani M, Pisaniello DL, Williams FM, Edwards JW (2001) Peripheral cholinesterase inhibition by occupational chlorpyrifos exposure in Australian termiticide applicators. Toxicology 169:177–185CrossRefGoogle Scholar
  8. Ellman GL, Courtney D, Andres VJ, Featherstone RM (1961) A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol 7:88–95CrossRefGoogle Scholar
  9. Gomes J, Lloyd O, Revitt MD, Basha M (1998) Morbidity among farm workers in a desert country in relation to long-term exposure to pesticides. Scand J Work Environ Health 24:213–219Google Scholar
  10. Hernández AF, Mackness B, Rodrigo L, Lopez O, Pla A, Gil F, Durrington PN, Pena G, Parron T, Serrano JL, Mackness MI (2003) Paraoxonase activity and genetic polymorphisms in greenhouse workers with long term pesticide exposure. Hum Exp Toxicol 22:565–574CrossRefGoogle Scholar
  11. Hernández AF, Gomez MA, Pena G, Gil F, Rodrigo L, Villanueva E, Pla A (2004) Effect of long-term exposure to pesticides on plasma esterases from plastic greenhouse workers. J Toxicol Environ Health A 67:1095–1108CrossRefGoogle Scholar
  12. Hernández AF, Lopez O, Rodrigo L, Gil F, Pena G, Serrano JL, Parron T, Alvarez JC, Lorente JA, Pla A (2005) Changes in erythrocyte enzymes in humans long-term exposed to pesticides: influence of several markers of individual susceptibility. Toxicol Lett 159:13–21CrossRefGoogle Scholar
  13. Hruska AJ, Corriols M (2002) The impact of training in integrated pest management among Nicaraguan maize farmers: increased net returns and reduced health risk. Int J Occup Environ Health 8:191–200Google Scholar
  14. Jors E, Cervantes R, Condarco G, Huici O, Lander F, Baelum J, Konradsen F (2006) Occupational pesticide intoxications among farmers in Bolivia: a cross-sectional study. Environ Health 5:10CrossRefGoogle Scholar
  15. Kamel F, Hoppin JA (2004) Association of pesticide exposure with neurologic dysfunction and disease. Environ Health Perspect 112:950–958Google Scholar
  16. Kashyap SK, Jani JP, Saiyed HN, Gupta SK (1984) Clinical effects and cholinesterase activity changes in workers exposed to phorate (Thimet). J Environ Sci Health B 19:479–489CrossRefGoogle Scholar
  17. Keifer MC (2000) Effectiveness of interventions in reducing pesticide overexposure and poisonings. Am J Prev Med 18(4 suppl):80–89CrossRefGoogle Scholar
  18. London L, Nell V, Thompson ML, Myers JE (1998) Effects of long-term organophosphate exposures on neurological symptoms, vibration sense and tremor among South African farm workers. Scand J Work Environ Health 24:18–29Google Scholar
  19. Mansour SA (2004) Pesticide exposure–Egyptian scene. Toxicology 198:91–115CrossRefGoogle Scholar
  20. Mekonnen Y, Ejigu D (2005) Plasma cholinesterase level of Ethiopian farm workers exposed to chemical pesticide. Occup Med (Lond) 55:504–505CrossRefGoogle Scholar
  21. Midtling JE, Barnett PG, Coye MJ, Velasco AR, Romero P, Clements CL, O’Malley MA, Tobin MW, Rose TG, Monosson IH (1985) Clinical management of field worker organophosphate poisoning. West J Med 142:514–518Google Scholar
  22. Misra UK, Nag D, Bhushan V, Ray PK (1985) Clinical and biochemical changes in chronically exposed organophosphate workers. Toxicol Lett 24:187–193CrossRefGoogle Scholar
  23. More PR, Vadlamudi VP, Degloorkar NM, Rajurkar SR (2003) Health monitoring of farm labourers engaged in MIPC 50 WP field sprays. J Environ Biol 24:205–209Google Scholar
  24. Ngowi AV, Maeda DN, Partanen TJ, Sanga MP, Mbise G (2001) Acute health effects of organophosphorus pesticides on Tanzanian small-scale coffee growers. J Expo Anal Environ Epidemiol 11:335–339CrossRefGoogle Scholar
  25. Ohayo-Mitoko GJ, Kromhout H, Simwa JM, Boleij JS, Heederik D (2000) Self reported symptoms and inhibition of acetylcholinesterase activity among Kenyan agricultural workers. Occup Environ Med 57:195–200CrossRefGoogle Scholar
  26. Recena MC, Caldas ED, Pires DX, Pontes ER (2006) Pesticides exposure in Culturama, Brazil–knowledge, attitudes, and practices. Environ Res 102:230–236CrossRefGoogle Scholar
  27. Richter ED, Chuwers P, Levy Y, Gordon M, Grauer F, Marzouk J, Levy S, Barron S, Gruener N (1992) Health effects from exposure to organophosphate pesticides in workers and residents in Israel. Isr J Med Sci 28:584–598Google Scholar
  28. Strong LL, Thompson B, Coronado GD, Griffith WC, Vigoren EM, Islas I (2004) Health symptoms and exposure to organophosphate pesticides in farmworkers. Am J Ind Med 46:599–606CrossRefGoogle Scholar
  29. Yucra S, Rubio J, Gasco M, Gonzales C, Steenland K, Gonzales GF (2006a) Semen quality and reproductive sex hormone levels in Peruvian pesticide sprayers. Int J Occup Environ Health 12:355–361Google Scholar
  30. Yucra S, Steenland K, Chung A, Choque F, Gonzales GF (2006b) Dialkyl phosphate metabolites of organophosphorus in applicators of agricultural pesticides in Majes-Arequipa (Peru). J Occup Med Toxicol 1:27CrossRefGoogle Scholar
  31. WHO-UNEP Working Group (1990) Public Health Impact of Pesticides Used in Agriculture. World Health Organization, Geneva, pp. 1–128Google Scholar

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Hector C. Cataño
    • 1
  • Elizabeth Carranza
    • 2
  • Carlos Huamaní
    • 3
  • Antonio F. Hernández
    • 4
  1. 1.Laboratorio de Biología Molecular, Instituto de Química Biológica, Microbiología y BiotecnologíaFacultad de Farmacia y Bioquímica, Universidad Nacional Mayor de San MarcosJardín BotánicoPerú
  2. 2.Laboratorio de Bioquímica. Instituto Nacional de Biología AndinaFacultad de Medicina, Universidad Nacional Mayor de San MarcosLimaPerú
  3. 3.Dirección Ejecutiva de Prevención y Control de Riesgos Ocupaciones y Ambientales, Centro Nacional de Salud Ocupacional y Protección del Ambiente para la Salud (CENSOPAS)Instituto Nacional de Salud (INS)LimaPeru
  4. 4.Department of Legal Medicine and ToxicologyUniversity of Granada Medical School18071-GranadaSpain

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