Acute and Chronic Toxicity of Fluoxetine (Selective Serotonin Reuptake Inhibitor) in Western Mosquitofish

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Abstract

Fluoxetine is a biologically active pharmaceutical chemical that has been detected at parts-per-trillion levels in surface waters in North America and Europe. This has generated concern because negative effects in aquatic organisms are possible. Known as a selective serotonin reuptake inhibitor, fluoxetine (e.g., Prozac; Elli Lilly) is neurologically active and widely prescribed for clinical depression in humans. In the present investigation, acute and chronic toxicities of fluoxetine were evaluated in an environmentally relevant species, western mosquitofish Gambusia affinis. Acute toxicity (5 to 5340 ppb fluoxetine) was assessed in neonates (age 24 to 48 hours) exposed in glass aquaria for 7 days; chronic toxicity (0.05 to 5 ppb fluoxetine) was examined in fish exposed from age neonate to age 91 days; and effects of chronic exposure (100 days) on sexual maturation was investigated in mesocosm tanks (100 L) in fish exposed (7 to 71 ppb) from age 59 to 159 days. Acute toxicity of fluoxetine in neonate western mosquitofish was observed to have a 7-day median lethal concentration of 546 ppb. Chronic exposure did not affect survival, growth, or sex ratio; however, increased lethargy in fish exposed to ≥0.5 ppb fluoxetine was observed. In fish exposed from age 59 to 159 days (juvenile to adult life stages), delayed development of external adult sexual morphology was observed at 71 ppb fluoxetine, which consisted of delayed onset of the presence of the black spot in the posterior abdomen in female fish and delayed formation of the elongated anal fin (gonopodium) in male fish. The present study demonstrated that chronic exposure of western mosquitofish to fluoxetine can affect sexual development; however, it does so only at concentrations 3 to 4 orders of magnitude higher than those previously found in the environment.

Keywords

Fluoxetine Black Spot Nominal Concentration Female Fish Male Fish 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References

  1. Brooks BW, Foran CM, Richards SM, Weston J, Turner PK, Stanley JK, et al. (2003) Aquatic ecotoxicology of fluoxetine. Toxicol Lett 142:169–183CrossRefGoogle Scholar
  2. Daughton CG, Ternes TA (1999). Pharmaceuticals and personal care products in the environment: Agents of subtle change? Environ Health Perspect 107:907–938CrossRefGoogle Scholar
  3. Fong PP, Huminski PT, D’Urso LM (1998) Induction and potentiation of parturition in fingernail clams (Sphaerium striatinum) by selective serotonin re-uptake inhibitors (SSRIs). J Exp Zool 280:260–264CrossRefGoogle Scholar
  4. Foran CM, Weston J, Slattery M, Brooks BW, Huggett DB (2004) Reproductive assessment of Japanese medaka (Oryzias latipes) following a four-week fluoxetine (SSRI) exposure. Arch Environ Contam Toxicol 46:511–517CrossRefGoogle Scholar
  5. Henry TB, Kwon JW, Armbrust KL, Black MC (2004). Acute and chronic toxicity of five selective serotonin reuptake inhibitors in Ceriodaphnia dubia. Environ Toxicol Chem 23:2229–2233CrossRefGoogle Scholar
  6. Huber R, Smith K, Delago A, Isaksson K, Kravitz EA (1997). Serotonin and aggressive motivation in crustaceans: Altering the decision to retreat. Proc Natl Acad Sci U S A 94:5939–5942CrossRefGoogle Scholar
  7. Jones AH, Voulvoulis N, Lester JN (2004) Potential ecological and human health risks associated with the presence of pharmaceutically active compounds in the aquatic environment. Crit Rev Toxicol 34:335–350CrossRefGoogle Scholar
  8. Kolpin DW, Furlong ET, Meyer MT, Thurman EM, Zaugg SD, Barber LB, et al. (2002) Pharmaceuticals, hormones, and other organic wastewater contaminants in U.S. streams, 1999–2000: A national reconnaissance. Environ Sci Technol 36:1202–1211CrossRefGoogle Scholar
  9. Metcalfe CD, Miao XS, Koenig BG, Struger J (2003) Distribution of acidic and neutral drugs in surface waters near sewage treatment plants in the lower Great Lakes, Canada. Environ Toxicol Chem 22:2881–2889CrossRefGoogle Scholar
  10. Overli O, Korzan WJ, Larson ET, Winberg S, Lepage O, Pottinger TG, et al. (2004) Behavioral and neuroendocrine correlates of displaced aggression in trout. Horm Behav 45:324–329CrossRefGoogle Scholar
  11. Perreault HAN, Semsar K, Godwin J (2003) Fluoxetine treatment decreases territorial aggression in a coral reef fish. Physiol Behav 79:719–724CrossRefGoogle Scholar
  12. Richards SM, Wilson CJ, Johnson DJ, Castle DM, Lam M, Mabury SA, et al. (2004) Effects of pharmaceutical mixtures in aquatic microcosms. Environ Toxicol Chem 23:1035–1042CrossRefGoogle Scholar
  13. Rosa-Molinar E, Proskocil BJ, Hendricks SE, Fritzsch B (1998) A mechanism for anterior transposition of the anal fin and its appendicular support in the western mosquitofish, Gambusia affinis affinis Baird and Girard, 1854. Acta Anatomica 163:75–91CrossRefGoogle Scholar
  14. Schloss P, Williams DC (1998) The serotonin transporter: A primary target for antidepressant drugs. J Psychopharmacol 12:115–121CrossRefGoogle Scholar
  15. Ternes TA (1998) Occurrence of drugs in German sewage treatment plants and rivers. Water Res 32:3245–3260CrossRefGoogle Scholar
  16. United States Environmental Protection Agency (1993) Methods for measuring the acute toxicity of effluents and receiving waters to freshwater and marine organisms, 4th edition. EPA/600/4-90/027F, Cincinnati, OHGoogle Scholar
  17. Zar JH (1984) Biostatistical analysis, 2nd edition. Prentice-Hall, Englewood Cliffs, NJGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  1. 1.Department of Environmental Health ScienceUniversity of GeorgiaAthensUSA

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