Heavy Metal Distribution in Tissues of Six Fish Species Included in Human Diet, Inhabiting Freshwaters of the Nature Park “Hutovo Blato” (Bosnia and Herzegovina)

  • Elizabeta Has-SchönEmail author
  • Ivan Bogut
  • Valentina Rajković
  • Stjepan Bogut
  • Milan Čačić
  • Janja Horvatić


The aim of the study was to quantify heavy metal (mercury, lead, cadmium, and arsenic) concentration in tissues (muscles, liver, kidney, gills, and gonads) of six fish species (carp: Cyprinus carpio, tench: Tinca tinca, pumpkinseed: Lepomis gibosus, prussian carp: Carassius auratus gibelio, hasselquist: Salmo dentex, eel: Anguilla anguilla) from the freshwaters of the Nature Park Hutovo Blato, Bosnia and Herzegovina, and determine whether they are potentially harmful for human health if included in the diet. Fish were angled from the Svitava Lake in the second part of August of the year 2003, and fish tissues were stored at −18°C until analysis. Heavy metal concentration was determined by atomic absorption spectrophotometry in the Veterinary Institute Brno, Czech Republic, and expressed as mg·kg−1 of wet tissue. Concentration of mercury, lead, and arsenic in most tissues of all analyzed fish types is lower than the maximal allowed concentration (MAC) in most countries. Cadmium concentration is also low in muscles and gonads, but kidney, liver, and gill concentrations exceed MAC value in most countries. Hasselquist, an endemic type for that region, differs from other fish types in the fact that it has very low cadmium concentration in liver and kidney, but the highest concentration of arsenic in most tissues, especially muscles. In muscles and gonads of all fish types analyzed, Pb is present in higher concentration than Cd, whereas in liver, gills, and particularly kidney, the situation is opposite, suggesting diverse metabolic pathways and unequal bioaccumulation of these two metals in different fish tissues. Although the region of the Nature Park Hutovo Blato in Bosnia and Herzegovina is not an agricultural territory, the intensive agricultural activities in the neighboring regions already result in high cadmium concentration in inner organs of fish species analyzed. Therefore, fish types in the freshwaters of the Park may be included in the human diet, but without inner organs and gills (or the whole head).


Mercury Lead Cadmium Arsenic Carp (Cyprinus carpio) Pumpkinseed (Lepomis gibosus) Eel (Anguilla anguilla) Tench (Tinca tinca) Prussian carp (Carassius auratus gibelio) Hasselquist (Salmo dentex) Hutovo blato 


  1. Barak NAE, Mason CF (1990) Mercury, cadmium and lead concentrations in five fish species of freshwater fish from eastern England. Sci Total Environ 92:257–263CrossRefGoogle Scholar
  2. Bogut I (1997) Water pollution by heavy metals and their impact on fish and human health. Hrvatske Vode 5:223–229Google Scholar
  3. Bogut I, Has-Schon E, Janson R, Antunović Z, Bodakoš D (2000) Concentrations of Pb, Hg, Cd, and As in meat of fish-pond carp (Cyprinus carpio). Papers of 8th International Symposium Animal Science Days, Osijek: 123–125Google Scholar
  4. Bogut I, Novoselić D (2005) Sistematika riba. In: Ribarstvo 1—Anatomija i fiziologija riba, sistematika riba, ekologija i zaštita riba, University of Osijek and Mostar, Grude, 179–403Google Scholar
  5. Bošnir J, Puntarić D, Šmit Z, Capuder Ž (1999) Fish as an indicator of eco-system contamination with mercury. Croat Med J 40:546–549Google Scholar
  6. Collings SE, Johnson MS, Leah RT (1996) Metal contamination of angler-caught fish from the Mersey Estuary. Mar Environ Res 41:281–297CrossRefGoogle Scholar
  7. Doganoc D (1995) Ostanki Pb, Cd, As in Hg v slatkovodnih in morskih ribah z območja Slovenije. Papers of the Scientific Meeting ‘‘Toxic Substances in Food and Object in Common Use,” pp 119–127Google Scholar
  8. Glantz SA (1997) How to test for differences between groups. In: Primer of biostatistics, 4th ed. McGraw-Hill, New York, pp 32–64Google Scholar
  9. Harada M (1995) Minamata disease: methylmercury poisoning in Japan caused by environmental pollution. Crit Rev Toxicol 25:1–24CrossRefGoogle Scholar
  10. Has-Schön E, Bogut I, Strelec I (2006) Heavy metal profile in five fish species included in human diet, domiciled in the end flow of River Neretva (Croatia). Arch Environ Contam Toxicol 50:545–551CrossRefGoogle Scholar
  11. Holčík J, Hensel K (1972) Handbook of ichthyology [in Slovak]. Obzor, Bratislava, Slovakia, p 217Google Scholar
  12. Horvat D, Ivezić M (2005) Analiza varijance. In: Biometrika u poljoprivredi, Poljoprivredni fakultet. Osijek, Croatia, pp 192–193Google Scholar
  13. Kudo A, Miyahara S (1984) Mercury dispersion from Minamata Bay to the Yatsushiro Sea during 1975–1980. EcotoxicoI Environ Saf 8:507–510CrossRefGoogle Scholar
  14. Máchová J, Svobodová Z, Hrjtmánek M, Hrbková M (1991) Control of hygienic quality of fish from the point of view of foreign substances content. In: Vykusová B (ed) Diagnostic, prevention and therapy of fish diseases and intoxications. Manual. Vodnany, Czech Republic, pp 325–445Google Scholar
  15. Martinović Đ, Koštrun M, Čaušić N (1994) Koncentracija teških metala u tkivima šarana. Papers of the Scientific Meeting “Metal in Food and Environment”, Stubičke toplice, pp 247–252Google Scholar
  16. Ministry of Health 1, Republic of Croatia (1994) By-law on the allowed concentrations of pesticides, toxins, myco-toxins, metals, and histamine and related substances in food, and on other safety conditions of food and necessities in large-scale use. Narodne novine no. 46, p 30Google Scholar
  17. Ministry of Health 2, Republic of Croatia (2001) By-law on modifications and amendments of the by-law on the allowed concentrations of pesticides, toxins, mycotoxins, metals, and histamine and related substances in food, and on other safety conditions of food and necessities in large-scale use. Narodne novine, no. 11, p 31Google Scholar
  18. Pourang N, Nikouyan A, Dennis JH (2005) Trace element concentrations in fish, surficial sediments and water from northern part of the Persian Gulf. Envir Monit Assess 109:293–316CrossRefGoogle Scholar
  19. Svobodova Z, Vykusova B, Machova J, Hrbkova M, Svobodnik J (1993) Monitoring of foreign substances in fishes from the Elbe river in the Ćelakovice locality. Bull VURH Vodnany 29:47–61Google Scholar
  20. Tsubaki TK (1977) Minamata disease: methyl mercury poisoning in Minamata and Niigata, Japan. Elsevier Scientific, AmsterdamGoogle Scholar
  21. Wayne WD (2005) Analysis of variance. In: Biostatistics, 8th ed. Hoboken, New Jersey, John Wiley & Sons, pp 303–320Google Scholar
  22. WHO/FAO (1985) Joint FAO/WHO standards programme. Codex committee on food additives. Information on legal and other administrative limits for contaminants in food. Hague, WHOGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Elizabeta Has-Schön
    • 1
    Email author
  • Ivan Bogut
    • 2
  • Valentina Rajković
    • 1
  • Stjepan Bogut
    • 3
  • Milan Čačić
    • 4
  • Janja Horvatić
    • 1
  1. 1.Department of BiologyJ.J. Strossmayer UniversityOsijekCroatia
  2. 2.Department of Animal HusbandryFaculty of Agriculture, J.J. Strossmayer UniversityOsijekCroatia
  3. 3.Health Centre GrudeGrudeBosnia and Herzegovina
  4. 4.Faculty of Food TechnologyJ.J. Strossmayer UniversityOsijekCroatia

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