Increased Thyroid Hormone Levels in Tree Swallows (Tachycineta bicolor) on Reclaimed Wetlands of the Athabasca Oil Sands

  • Marie-Line Gentes
  • Anne McNabb
  • Cheryl Waldner
  • Judit E. G. Smits


The oil sands of Alberta, Canada are one of the world’s largest reserves of crude oil. Oil sands mining companies are now investigating the ecological impacts of reclamation strategies in which wetlands are used for the bioremediation of waste materials. To examine the endocrine disrupting potential of chemicals in Oil Sands Process Materials (OSPM), thyroid hormone concentrations were measured in plasma and thyroid glands of nestling tree swallows (Tachycineta bicolor) from wetlands partly filled with mine tailings. Plasma triiodothyronine (T3) concentrations and thyroxine (T4) content within thyroid glands were elevated in nestlings from OSPM sites compared to those from the reference site. Results suggested enhanced hormone synthesis by the thyroid glands independently of activation of the pituitary–thyroid axis, as well as increased deiodination of T4 into T3 in peripheral tissues. This might have resulted from exposure to oil sands associated chemicals such as polycyclic aromatic hydrocarbons and from environmental factors such as food availability. Modulation of thyroid function might have negative effects on metabolism, behavior, feather development, and molt, which could compromise postfledging survival.


  1. Albers PH (2003) Petroleum and individual polycyclic aromatic hydrocarbons. In: Hoffman DJ, Rattner BA, Burton GA, Cairns J (eds) Handbook of ecotoxicology. CRC, Boca Raton, FL, pp 341–371Google Scholar
  2. Alberta Department of Energy. (2005). Oil Sands. Available from
  3. Bendell-Young LI., Bennett KE, Crowe A, Kennedy CJ, Kermode AR, Moore MM, Plant AL, Wood A (2000) Ecological characteristics of wetlands receiving an industrial effluent. Ecol Applic 10:310–322Google Scholar
  4. Brown SB, Adams BA, Cy DG, Eales JG (2004) Contaminant effects on the teleost fish thyroid. Environ Toxicol Chem 23:1680–1701CrossRefGoogle Scholar
  5. Clemente JS, Fedorak PM (2005) A review of the occurence, analyses, toxicity and biodegradation of naphthenic acids. Chemosphere 60:585–600CrossRefGoogle Scholar
  6. Dawson A, King VM, Bentley GE, Ball GF (2001) Photoperiodic control of seasonality in birds. J Biol Rhythms 16:365–380CrossRefGoogle Scholar
  7. Fox GA (2001) Effects of endocrine disrupting chemicals on wildlife in Canada: Past, present and future. Water Qual Res J Canada 36:233–251Google Scholar
  8. FTFC (Fine Tailing Fundamental Consortium) (1995) Fine tails and process water reclamation. In: Advances in Oil Sands Tailing Research. Alberta Department of Energy, Oil Sands Research Division, Edmonton, pp 1–52Google Scholar
  9. Ganshorn KD (2002) Secondary production, trophic position, and potential for accumulation of polycyclic hydrocarbons in predatory diptera in four wetlands of the Athabasca Oil Sands, Alberta, Canada. MSc thesis, University of Windsor, Windsor, CanadaGoogle Scholar
  10. Gentes M-L, Waldner C, Papp Z, Smits JE (2006) Effects of oil sands tailing compounds and harsh weather on mortality rates, growth and detoxification efforts in nestling tree swallows (Tachycineta bicolor). Environ Pollut 142:24–33CrossRefGoogle Scholar
  11. Harvey S, Klandorf H, Phillips JG (1981) Reproductive performance and endocrine responses to ingested petroleum in domestic ducks (Anas-Platyrhynchos). Gen Comp Endocrinol 45:372–380CrossRefGoogle Scholar
  12. Hox JJ (2002) Multilevel analysis: Techniques and applications. Lawrence Erlbaum Associates, Mahwah, NJGoogle Scholar
  13. Jenssen BM, Ekker M, Zahlsen K (1990) Effects of ingested crude-oil on thyroid hormones and on the mixed-function oxidase system in ducks. Comp Biochem Physiol C: Pharmacol Toxicol Endocrinol 95 213–216Google Scholar
  14. Jones J (2003) Tree swallows (Tachycineta bicolor): a new model organism? Auk 120:591–599CrossRefGoogle Scholar
  15. Leung SS, MacKinnon MD, Smith REH (2003) The ecological effects of naphthenic acids and salt on phytoplankton from the Athabasca oil sands region. Aquatic Toxicol 62:11–26CrossRefGoogle Scholar
  16. Lothrop C.D. (1996) Diseases of the endocrine system. In: Rosskopf WJ, Woerpel RW (eds) Diseases of cage and aviary birds. Williams and Wilkins, Baltimore, MD, pp 368–379Google Scholar
  17. Madill REA, Orzechowski MT, Chen G, Brownlee BG, Bunce NJ (2001) Preliminary risk assessment of the Wet Landscape option for reclamation of Oils Sands mine tailings:bioassays with mature fine tailings pore water. Environ Toxicol 16:197–208CrossRefGoogle Scholar
  18. Mayne G.J, Bishop CA, Martin PA, Boermans HJ, Hunter B (2005) Thyroid function in nestling tree swallows and eastern bluebirds exposed to non-persistent pesticides and p,p ‘-DDE in apple orchards of southern Ontario, Canada. Ecotoxicol 14:381–396CrossRefGoogle Scholar
  19. Mcarthur MLB, Fox GA, Peakall DB, Philogene BJR (1983) Ecological significance of behavioral and hormonal abnormalities in breeding ring doves fed an organochlorine chemical mixture. Arch Environ Contam Toxicol 12:343–353CrossRefGoogle Scholar
  20. McNabb FMA (2000) Thyroids. In: Whittow GC (ed) Sturkie’s avian physiology. Academic Press, San Diego, pp 461–471Google Scholar
  21. McNabb FMA, (2005) Biomarkers for the assessment of avian thyroid disruption by chemical contaminants. Avian Poult Biol Rev 16:3–10CrossRefGoogle Scholar
  22. McNabb FMA, Cheng M-F (1985) Thyroid development in ring doves, Streptopelia risoria. Gen Comp Endocrinol 58:243–251CrossRefGoogle Scholar
  23. McNabb FMA, King DB (1993) Thyroid hormone effects on growth, development and metabolism. In: Schreibman MP, Scanes CG, Pang PKT (eds) The endocrinology of growth, development, and metabolism of vertebrates. Academic Press, New York, pp 393–417 Sturkie’s avian physiologyGoogle Scholar
  24. McNabb FMA, Larsen CT, Pooler PS (2004) Ammonium perchlorate effects on thyroid function and growth in bobwhite quail chicks. Environmental Toxicology and Chemistry 23: 997–1003CrossRefGoogle Scholar
  25. Neff JM (1985) Polycyclic aromatic hydrocarbons. In: Rand GM, Petrocelli SR (eds) Fundamentals of aquatic toxicology: Methods and applications. Hemisphere, New York, pp 666 Google Scholar
  26. Peakall DB, Tremblay J, Kinter WB, Miller DS (1981) Endocrine dysfunction in seabirds caused by ingested oil. Environ Res 24:6–14CrossRefGoogle Scholar
  27. Pollet I, Bendell-Young LI (2000) Amphibians as indicators of wetland quality in wetlands formed from Oil Sands effluents. Environmental Toxicology and Chemistry 19: 2589–2597CrossRefGoogle Scholar
  28. Rae M (2000) Avian endocrine disorders. In: Fudge AM (ed) Laboratory medicine: Avian and exotic pets. WB Saunders, PhiladelphiaGoogle Scholar
  29. Rolland RM (2000) A review of chemically-induced alterations in thyroid and vitamin A status from field studies of wildlife and fish. J Wildl Dis 36:615–635Google Scholar
  30. Scanes CG, Griminger P (1990) Endocrine-nutrition interactions in birds. J Exp Zool 256:98–105CrossRefGoogle Scholar
  31. Scanes CG, McNabb FMA (2003) Avian models for research in toxicology and endocrine disruption. Avian Poult Biol Rev 14:21–52Google Scholar
  32. Silva JE (1995) Thyroid hormone control of thermogenesis and energy balance. Thyroid 5:481–492CrossRefGoogle Scholar
  33. Singh H (1989) Interaction of xenobiotics with reproductive endocrine functions in a protogynous teleost, Monopterus albus. Marine Environ Res 28:285–289CrossRefGoogle Scholar
  34. Smits J.E., Wayland M.M., Miller M.J., Liber K., Trudeau S. (2000) Reproductive, immune,and physiological end points in tree swallows on reclaimed oil sands mine sites. Environmental Toxicology and Chemistry 19: 2951–2960CrossRefGoogle Scholar
  35. Stephens SM, Alkindi AYA, Waring CP, Brown JA (1997) Corticosteroid and thyroid responses of larval and juvenile turbot exposed to the water-soluble fraction of crude oil. J Fish Biol 50:953–964CrossRefGoogle Scholar
  36. van den Heuvel MR, Power M, Richards J, MacKinnon MD, Dixon DG (2000) Disease and gill lesions in yellow perch (Perca flavescens) exposed to Oil Sands mining-associated waters. Ecotoxicol Environ Safety 46:334–341CrossRefGoogle Scholar
  37. Wayland M, Smits JE (2003) The ecological viability of constructed wetlands at Suncor: population and health-related considerations in birds. Assessment of natural and anthropogenic impacts of oil sands contaminants within the northern river basins. Final summary report -Task 5: Hydrocarbons/oil sands and heavy oil research and development. Environment CanadaGoogle Scholar
  38. Whelly MP (1999) Aquatic invertebrates in wetlands of the Oil Sands region of northeast Alberta, Canda, with emphasis on Chironomidae (Diptera). MSc thesis. University of Windsor, CanadaGoogle Scholar
  39. Wilson CM, McNabb FMA (1997) Maternal thyroid hormones in Japanese quail eggs and their influence on embryonic development. General and Comparative Endocrinology. 107:153–165CrossRefGoogle Scholar
  40. Woodhead AD, Setlow RB, Pond V (1982) Effects of polycyclic aromatic hydrocarbons on the proliferation of ectopic thyroid tissue in Poecilia formosa, the Amazon Molly. J Fish Biology 20:455–463CrossRefGoogle Scholar
  41. York RG, Brown WR, Girard MF, Dollarhide JS (2001) Two-generation reproduction study of ammonium perchlorate in drinking water in rats evaluates thyroid toxicity. Int J Toxicol 20:183–197 CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Marie-Line Gentes
    • 1
  • Anne McNabb
    • 2
  • Cheryl Waldner
    • 3
  • Judit E. G. Smits
    • 1
  1. 1.Department of Veterinary PathologyUniversity of SaskatchewanSaskatoonCanada
  2. 2.Department of Biological SciencesVirginia TechBlacksburgUSA
  3. 3.Department of Large Animal Clinical SciencesUniversity of SaskatchewanSaskatoonCanada

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