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Uptake and Elimination of Chlorpyrifos and Pentachlorophenol into the Freshwater Amphipod Gammarus pulex

  • Roman Ashauer
  • Alistair Boxall
  • Colin Brown
Article

Abstract

Uptake and elimination rates were determined for chlorpyrifos (CPF) and pentachlorophenol (PCP) in the freshwater amphipod Gammarus pulex. Internal concentrations of the two pesticides were measured over a three-day exposure phase and a subsequent three-day elimination phase. Rate constants were obtained by fitting measured internal concentrations to a one-compartment single first-order model. The uptake rate constants were 747 ± 61 [L kg−1 day−1] for CPF and 89 ± 7 [L kg−1 day−1] for PCP. The elimination rate constants were 0.45 ± 0.05 [day−1] for CPF and 1.76 ± 0.14 [day−1] for PCP. The resulting bioconcentration factors at steady state were 1660 and 51 for CPF and PCP, respectively. The parameter estimation method and possible variability due to varying lipid content are briefly discussed.

Keywords

Lipid Content Elimination Rate Chlorpyrifos Leaf Material Elimination Rate Constant 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work was funded under the EU framework VI programme, project number SSPE-CT-2003-501997 (HAIR: Harmonised environmental Indicators for Pesticide Risk).

References

  1. Crane M, Maltby L (1991) The lethal and sublethal responses of Gammarus pulex to stress: sensitivity and sources of variation in an in situ bioassay. Environ Toxicol Chem 10:1331–1339Google Scholar
  2. Deneer JW (1993) Uptake and elimination of chlorpyrifos in the guppy at sublethal and lethal aqueous concentrations. Chemosphere 26(9):1607–1616CrossRefGoogle Scholar
  3. Gee JHR (1988) Population dynamics and morphometrics of Gammarus pulex L.: evidence of seasonal food limitation in a freshwater detritivore. Freshwater Biol 19:333–343CrossRefGoogle Scholar
  4. Gobas FAPC, Morrison HA (2000) Bioconcentration and biomagnification in the Aquatic Environment. In: Boethling RS, Mackay D, (eds), Handbook of property estimation methods for chemicals. CRC Press, Boca Raton 189–231Google Scholar
  5. Handy RD (1994) Intermittent exposure to aquatic pollutants: assessment, toxicity and sublethal responses in fish and invertebrates. Comp Biochem Phys C 107(2):171–184Google Scholar
  6. Hendriks JA (1995a) Modelling non-equilibrium concentrations of microcontaminants in organisms: Comparative kinetics as a function of species size and octanol-water partitioning. Chemosphere 30(2):265–292CrossRefGoogle Scholar
  7. Hendriks AJ (1995b) Modelling equilibrium concentrations of microcontaminants in organisms of the Rhine delta: Can average field residues in the aquatic foodchain be predicted from laboratory accumulation? Aquat Toxicol 31(1):1–25CrossRefGoogle Scholar
  8. Kaiser KLE, Valdmanis I (1982) Apparent octanol/water partition coefficients of pentachlorophenol as a function of pH. Can J Chem 60:2104–2106CrossRefGoogle Scholar
  9. Kooijman SALM, Bedaux JJM (1996) The analysis of aquatic toxicity data. VU University Press, Amsterdam, NetherlandsGoogle Scholar
  10. Kuhn K, Streit B (1994) Detecting sublethal effects of organophosphates by measuring acetylcholinesterase activity in Gammarus. B Environ Contam Tox 53(3):398–404CrossRefGoogle Scholar
  11. Landrum PF, Dupuis WS (1990) Toxicity and toxicokinetics of pentachlorophenol and carbaryl to Pontoporeia hoyi and Mysis relicta. In: Landis WG, van der Schalie WH (eds) Aquatic toxicology and risk assessment. Philadelphia: American Society for Testing and Materials, pp 278–289Google Scholar
  12. Landrum PF, Lee HI, Lydy MJ (1992) Toxicokinetics in aquatic systems: model comparisons and use in hazard assessment. Environ Toxicol Chem 11:1709–1725Google Scholar
  13. Lotufo GR, Landrum PF, Gedeon ML, Tigue EA, Herche LR (2000) Comparative toxicity and toxicokinetics of DDT and its major metabolites in freshwater amphipods. Environ Toxicol Chem 19(2):368–379CrossRefGoogle Scholar
  14. Maltby L, Clayton SA, Wood RM, McLoughlin N (2002) Evaluation of the Gammarus pulex in situ feeding assay as a biomonitor of water quality: robustness, responsiveness and relevance. Environ Toxicol Chem 21(2):361–368CrossRefGoogle Scholar
  15. McCarty LS, Mackay D (1993) Enhancing ecotoxicological modeling and assessment: body residues and modes of toxic action. Environ Sci Technol 27:1719–1728CrossRefGoogle Scholar
  16. McLoughlin N, Yin D, Maltby L, Wood RM, Yu H (2000) Evaluation of sensitivity and specificity of two crustacean biochemical biomarkers. Environ Toxicol Chem 19(8):2085–2092CrossRefGoogle Scholar
  17. Modelkinetix (2000): Modelmaker reference. Cherwell Scientific Ltd., OxfordGoogle Scholar
  18. Montanes JFC, van Hattum B, Deneer J (1995) Bioconcentration of chlorpyrifos by the freshwater isopod Asellus aquaticus (L.) in outdoor experimental ditches. Environ Pollut 88(2):137–146CrossRefGoogle Scholar
  19. Nuutinen S, Landrum PF, Schuler LJ, Kukkonen JVK, Lydy MJ (2003) Toxicokinetics of organic contaminants in Hyalella azteca. Arch Environ Con Tox 44(4):467–475CrossRefGoogle Scholar
  20. Penttinen O-P, Kukkonen J, (1998). Chemical stress and metabolic rate in aquatic invertebrates: threshold, dose-response relationships, and mode of toxic action. Environmental Toxicology and Chemistry 17(5):883–890CrossRefGoogle Scholar
  21. Reinert KH, Giddings JM, Judd L. (2002) Effects analysis of time-varying or repeated exposures in aquatic ecological risk assessment of agrochemicals. Environ Toxicol Chem 21:1977–1992CrossRefGoogle Scholar
  22. Roberts TR, Hutson DH, Jewess PJ, Lee PW, Nicholls PH, Plimmer JR (1999): Metabolic pathways of agrochemicals. Part 2: Insecticides and fungicides. The Royal Society of Chemistry, Cambridge, UKGoogle Scholar
  23. Serrano R, Lopez FJ, Hernandez F, Pena JB (1997) Bioconcentration of chlorpyrifos, chlorfenvinphos, and methidathion in Mytilus galloprovincialis. B Environ Contam Tox 59(6):968–975CrossRefGoogle Scholar
  24. Slooff W (1983) Benthic macroinvertebrates and water quality assessment: some toxicological considerations. Aquat Toxicol 4:73–82CrossRefGoogle Scholar
  25. Steevens JA, Benson WH (2001) Toxicokinetic interactions and survival of Hyalella azteca exposed to binary mixtures of chlorpyrifos, dieldrin, and methyl mercury. Aquat Toxicol 51(4):377–388CrossRefGoogle Scholar
  26. Sutcliffe DW, Carrick TR, Willoughby LG (1981) Effects of diet, body size, age and temperature on growth rates in the amphipod Gammarus pulex. Freshwater Biol 11:183–214CrossRefGoogle Scholar
  27. Tomlin CDS (2000) The pesticide manual, 12 ed. British Crop Protection Council Fornham, UKGoogle Scholar
  28. van den Brink PJ, van Donk E, Gylstra R, Crum SJH, Brock TCM (1995) Effects of chronic low concentrations of the pesticides chlorpyrifos and atrazine in indoor freshwater microcosms. Chemosphere 31(5):3181–3200CrossRefGoogle Scholar
  29. van Wijngaarden RPA, van den Brink PJ, Crum SJH, Oude Voshaar JH, Brock TCM, Leeuwangh P (1996) Effects of the insecticide Dursban 4E (active ingredient chlorpyrifos) in outdoor experimental ditches: I. Comparison of short-term toxicity between the laboratory and the field. Environ Toxicol Chem 15(7):1133–1142CrossRefGoogle Scholar
  30. Wang X, Zauke GP (2004) Size-dependent bioaccumulation of metals in the amphipod Gammarus zaddachi (Sexton 1912) from the River Hunte (Germany) and its relationship to the permeable body surface area. Hydrobiologia 151:11–28CrossRefGoogle Scholar
  31. Westall JC, Leuenberger C, Schwarzenbach RP (1985) Influence of pH and ionic strength on the aqueous-nonaqueous distribution of chlorinated phenols. Environ Sci Technol 19(2):193–198CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Roman Ashauer
    • 1
    • 2
  • Alistair Boxall
    • 1
    • 2
  • Colin Brown
    • 1
    • 2
  1. 1. Environment DepartmentUniversity of YorkYorkUnited Kingdom
  2. 2. Central Science Laboratory, Sand HuttonYorkUnited Kingdom

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