Degradation of Aromatic Hydrocarbons by Sphingomonas paucimobilis Strain EPA505

  • S. P. Story
  • E. L. Kline
  • T. A. Hughes
  • M. B. Riley
  • S. S. Hayasaka

Abstract

To determine the substrate range capability of Sphingomonas paucimobilis strain EPA505, a number of aromatic compounds were tested as potential growth substrates. Strain EPA505 grew on phenanthrene, naphthalene, fluoranthene, toluene, benzoic acid, 2,3- and 3,4-dihydroxybenzoic acids, 1-chloro-2,4-dinitrobenzene, anthracene, 2-hydroxy-3-naphthoic acid and 1-hydroxy- 2-naphthoic acid, salicylic acid, and catechol. Strain EPA505 was unable to grow on coumarine 3-carboxylic acid, naphthalene dicarboxylic acid, acenaphthene, chrysene, pyrene, benzo[b]fluoranthene, and fluorene. Catabolic products were not detected or identified when the bacterium was incubated with coumarine 3-carboxylic acid, naphthalene dicarboxylic acid, acenaphthene, chrysene, or benzo[b]fluoranthene. Dihydroxypyrene, the ortho ring fission product of pyrene, and 10-hydroxy-1- phenanthroic acid were detected when the bacterium was incubated with pyrene. The open rings of benzo[b]fluoranthene, hydroxyacephenanthroic acid, hydroxyacephenanthrene, and phenanthrene anhydride, catabolites of benzo[b]fluoranthene degradation, were detected with Tn5 mutants of EPA505. With strain EPA505, both 9-fluorenone and an open ring fission product accumulated during incubation with fluorene. Other catabolites beyond the open ring of fluorene were detected, specifically dihydroxyfluorene, hydroxy-9-fluorenone, dihydroxy-9-fluorenone, hydroxyindane, and a putative glutathione-conjugated benzylanhydride. Benzylanhydride appeared to be a final end product of fluorene degradation by strain EPA505.

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Copyright information

© Springer-Verlag New York Inc. 2004

Authors and Affiliations

  • S. P. Story
    • 1
  • E. L. Kline
    • 1
  • T. A. Hughes
    • 1
  • M. B. Riley
    • 2
  • S. S. Hayasaka
    • 1
  1. 1.Department of Biological Sciences/Microbiology, Clemson University, Clemson, South Carolina 29634-0314USA
  2. 2.Department of Plant Pathology and Physiology, Clemson University, Clemson, South Carolina 29634-0315USA

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