Advertisement

Urological Research

, Volume 29, Issue 4, pp 272–277 | Cite as

Simultaneous monitoring of extracellular glucose, pyruvate, and lactate in rat testies during ischemia: a microdialysis study

  • Chen-Li Cheng
  • Chi-Re Yang
  • Dar-Yu Yang
  • Jon-Son Kuo
  • Yi-Fang Huang
  • Fu-Chou Cheng
Original Paper

Abstract.

A dual-probe microdialysis technique was developed for the simultaneous monitoring of glucose and related metabolite levels during testicular ischemia in rats. The determinations of lactate and pyruvate were achieved by liquid chromatography within 5 min. Glucose was determined by a microdialysis analyzer. A unilateral ligation was produced by occlusion of the right artery for 2 or 4 h in anesthetized rats. Microdialysis probes were inserted in both sides of the testis to simultaneously monitor glucose, lactate and pyruvate during basal, ischemia and reperfusion periods. Dynamic and comparative changes in these analytes in ipsilateral and contralateral testes were demonstrated. The present technique can be used as a tool for exploring energy related metabolites and their relationships in testicular ischemia.

Dual-probe Glucose Ischemia Lactate Microdialysis Pyruvate Testes 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  • Chen-Li Cheng
    • 1
  • Chi-Re Yang
    • 1
  • Dar-Yu Yang
    • 2
  • Jon-Son Kuo
    • 3
  • Yi-Fang Huang
    • 1
  • Fu-Chou Cheng
    • 3
  1. 1.Division of Urology, Department of Surgery, Taichung Veterans General Hospital, Taichung, 407Taiwan
  2. 2.Department of Emergency, Taichung Veterans General Hospital, Taichung, 407Taiwan
  3. 3.Department of Medical Research, Taichung Veterans General Hospital, Taichung, 407Taiwan

Personalised recommendations