Advertisement

Urological Research

, Volume 29, Issue 4, pp 282–286 | Cite as

The effect of osteopontin immobilized collagen granules in the seed crystal method

  • T. Umekawa
  • M. Iguchi
  • T. Kurita
Original Paper

Abstract.

Osteopontin (OPN) is a urinary protein which inhibits calcium oxalate (CaOx) crystal growth in the seed crystal system. The aim of this study was to evaluate the effects of OPN immobilized on collagen granules (CG) for CaOx crystal growth and aggregation in this system. OPN-immobilized CG showed a 30% decrease in inhibitory activity compared with non-OPN-immobilized CG. Scanning electron microscopy revealed that 1) OPN-immobilized CG showed more marked aggregation and adhesion of seed crystals (smaller than 0.5 µm in diameter) than non-OPN-immobilized CG, 2) the amount of adherent large type crystals (2–3 µm in diameter) did not differ between OPN immobilized and non-OPN-immobilized CG. In conclusion, immobilized OPN promotes aggregation and adhesion of CaOx crystals smaller than 0.5 µm on CG. These observations suggest that crystal adhesion to immobilized OPN plays a role in the retention of crystals in the kidney.

Osteopontin Collagen Seed crystal method Calcium oxalate 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  • T. Umekawa
    • 1
  • M. Iguchi
    • 2
  • T. Kurita
    • 2
  1. 1.4700 S.W., Archer Road, #S128 Gainesville, FL 32608USA
  2. 2.Department of Urology, Kinki University School of Medicine, 377-2 Ohno-Higashi, Osaka-Sayama, Osaka 589-0073Japan

Personalised recommendations