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Urological Research

, Volume 29, Issue 4, pp 287–292 | Cite as

E-cadherin expression and PSA secretion in human prostate epithelial cells

  • Diane Krill
  • Angela Thomas
  • Su-Ping Wu
  • Rajiv Dhir
  • Michael J. Becich
Original Paper

Abstract.

Prostate-specific antigen (PSA) is the most widely used marker for the diagnosis of prostate cancer and is an independent predictor of prostatic capsular invasion. A number of studies have identified E-cadherin, a cell adhesion protein, as a potential invasion suppressor which is decreased in prostate adenocarcinoma. Our goal in the present study was to evaluate E-cadherin expression in primary cultures and determine the relationship between E-cadherin expression and PSA secretion in both primary cultures and the prostate tumor cell line, LNCaP. Immunohistochemical studies and Western blot analysis confirmed greater expression of E-cadherin in normal epithelial cells than tumor-derived prostate cells. This is the first report that the incubation of normal prostate epithelial cells with E-cadherin antibody increases the amount of PSA detected in the media of normal cells as well as in LNCaP. Since E-cadherin may function as an invasion suppressor, an understanding of the decreased expression of this adhesion factor and the impact on PSA secretion may aid in understanding epithelial tumorigenesis.

PSA E-cadherin Prostate Human Cell culture 

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Copyright information

© Springer-Verlag 2001

Authors and Affiliations

  • Diane Krill
    • 1
  • Angela Thomas
    • 2
  • Su-Ping Wu
    • 2
  • Rajiv Dhir
    • 2
  • Michael J. Becich
    • 2
  1. 1.University of Pittsburgh School of Medicine, C920 Presbyterian University Hospital, 200 Lothrop Street, Pittsburgh, PA 15261USA
  2. 2.Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261USA

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