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Protective effect of pentoxifylline on oxidative renal cell injury associated with renal crystal formation in a hyperoxaluric rat model

  • Hayrettin Ozturk
  • Ayhan Cetinkaya
  • Tulin Siviloglu Firat
  • Buket Kin Tekce
  • Selma Erdogan Duzcu
  • Hulya Ozturk
Original Paper


The aim of the study is to investigate the effects of pentoxifylline (PTX) on the renal tubular cell injury and stone formation in a hyperoxaluric rat model induced by ethylene glycol and its possible underlying mechanisms. The study was performed with 30 male Wistar rats and randomized into three groups of teen. The sham-control (group 1) received only drinking water orally. The EG/untreated (group 2) received drinking water containing 0.75% EG for 4 weeks orally. The EG/PTX treated (group 3) received drinking water containing 0.75% EG for 4 weeks orally and PTX. Urine and blood were collected to determine some parameters. The kidneys were also removed for histological examination. Serum and urinary parameters were significantly improved in the EG/PTX treated. In the EG/PTX-treated group, the MDA, TOS and MPO activity reduced and the TAS, SOD, CAT and GSH-Px activities were increased markedly compared with the group 2. In urine of the group 2 rats, a large number of CaOx crystals were displayed and most tubules that contained crystals were dilated and showed degeneration, necrosis, and desquamation of the lining epithelium. Only few CaOx crystals were r in EG/PTX-treated animal’s urine. Mild tissue damage was observed in PTX-treated rats. iNOS expression was significantly elevated in the group 2. In contrast, in the EG/PTX-treated group, eNOS expression in renal tubular epithelial cells was increased. Current study indicates that PTX may partially reduce renal tubular injury resulting from hyperoxaluria-induced oxidative and nitrosative stress.


Experimental nephrocalcinosis Ethylene glycol Pentoxifylline Oxidative stress Rat 


Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Pediatric Surgery, Medical SchoolAbant Izzet Baysal UniversityBoluTurkey
  2. 2.Department of Physiology, Medical SchoolAbant Izzet Baysal UniversityBoluTurkey
  3. 3.Department of Histology and Embryology, Medical SchoolAbant Izzet Baysal UniversityBoluTurkey
  4. 4.Department of Biochemistry, Medical SchoolAbant Izzet Baysal UniversityBoluTurkey
  5. 5.Department of Pathology, Medical SchoolAbant Izzet Baysal UniversityBoluTurkey

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