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Urolithiasis

, Volume 47, Issue 2, pp 137–148 | Cite as

Electron probe micro-analysis reveals the complexity of mineral deposition mechanisms in urinary stones

  • Muhammed A. P. Manzoor
  • M. Mujeeburahiman
  • Punchappady-Devasya RekhaEmail author
Original Paper
  • 375 Downloads

Abstract

Urinary stones are complex mineralogical formations in the urinary system often impairing the kidney function. Several studies have attempted to understand the mechanisms of stone formation and growth; however, it remains to be fully explored. Here, we present a detailed investigation on the morphological and mineralogical characterizations of urinary stones. Structural properties of different types of urinary stones were done by X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), and field-emission scanning electron microscope (FE-SEM) analyses. X-ray maps of major and the trace elements were obtained using electron microprobe (EPMA) technique. Basic metabolic panel and urinary parameters of the patients were used for comparing mineral compositions among stone types. The study included five major types of stones identified based on the FTIR spectra. FTIR and XRD helped in identifying the major components of these stones. FE-SEM images revealed distinct microstructure and morphology of the stones among the stone types. EPMA analysis showed the presence of many metals other than calcium and certain non-metals within the urinary stone matrix at measurable levels, sometimes with distinct distribution patterns. The study demonstrates the characteristic micro-structure, morphology, distribution, and composition of elements in different stone types. Findings of the study provide scope for understanding the complex mechanisms involved in the urolithogenesis and association of trace elements in it.

Keywords

Urolithiasis Electron probe micro-analysis FTIR FE-SEM XRD Mixed stone 

Notes

Funding

This research received no specific grant from any funding agency in the public, commercial or not-for-profit sectors.

Compliance with ethical standards

Conflict of interest

None declared.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. Ethical clearance approval number YUEC.022/16.

Informed consent

Informed consent was obtained from all individual participants included in the study.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Muhammed A. P. Manzoor
    • 1
    • 2
  • M. Mujeeburahiman
    • 2
  • Punchappady-Devasya Rekha
    • 1
    Email author
  1. 1.Yenepoya Research CentreYenepoya UniversityMangaloreIndia
  2. 2.Department of Urology, Yenepoya Medical CollegeYenepoya UniversityMangaloreIndia

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