, Volume 46, Issue 6, pp 549–558 | Cite as

Impact of repeated extracorporeal shock wave lithotripsy on prepubertal rat kidney

  • Jae Min Chung
  • Bu Kyung Park
  • Jung Hee Kim
  • Hyun Jung Lee
  • Sang Don LeeEmail author
Original Paper


This study aimed to investigate the effects of repeated extracorporeal shock wave lithotripsy (ESWL) on the kidneys of prepubertal and adult rats. Thirty rats were used: 15 were prepubertal (3 weeks of age) with an average body weight of 72.3 ± 3.3 g, and 15 were adults with of 265 ± 11.3 g. The prepubertal and adult rats were separately and randomly allocated to three groups, each consisting of five rats. Following anesthetization, the left kidney of each rat in each group received shock waves in one, two, or three sessions separated by 72 h. The rats in each group were killed 72 h after the last ESWL session, and both kidneys were harvested; the right kidney was used as the control. Renal injury was examined with histological analysis, immunohistochemistry, and Western blot to detecting the expression of heat-shock protein-70, tumor necrosis factor-alpha-α, intercellular adhesion molecule-1, and monocyte chemoattractant protein-1 as markers of renal damage. All of these markers were similarly increased with increased ESWL sessions in both age groups. Histological analysis revealed more serious fibrosis and inflammation in the ESWL-treated kidneys in both groups than in the controls, with the damage increasing with increasing numbers of sessions. ESWL on the kidney increased renal damage according to the number of sessions in both age groups of rats, and the effects of ESWL on renal injury were similar in the two groups. However, there were generally no significant differences in the effects of ESWL on molecular indicators of renal injury between prepubertal and adult rats.


Inflammatory renal damage Shock wave Puberty Adult 



This study was supported by the Research Institute for Convergence of Biomedical Science and Technology (30-2014-005), Pusan National University Yangsan Hospital.

Compliance with ethical standards


There were no external sources of funding.

Author disclosure statement

No competing financial interests exist.

Conflict of interest

Author Jae Min Chung declares that he has no conflict of interest. Author Bu Kyung Park declares that he has no conflict of interest. Author Jung Hee Kim declares that she has no conflict of interest. Author Hyun Jung Lee declares that she has no conflict of interest. Author Sang Don Lee declares that he has no conflict of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed (PNUIACUC; PNU-2014-0612).


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Copyright information

© Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  1. 1.Department of UrologyPusan National University School of MedicineYangsanKorea
  2. 2.Research Institute for Convergence of Biomedical Science and TechnologyPusan National University Yangsan HospitalYangsanKorea
  3. 3.Department of PathologyPusan National University Yangsan HospitalYangsanKorea

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