, Volume 46, Issue 2, pp 179–186 | Cite as

Metabolic syndrome contributes to renal injury mediated by hyperoxaluria in a murine model of nephrolithiasis

  • Javier Sáenz-MedinaEmail author
  • E. Jorge
  • C. Corbacho
  • M. Santos
  • A. Sánchez
  • P. Soblechero
  • E. Virumbrales
  • E. Ramil
  • M. J. Coronado
  • I. Castillón
  • D. Prieto
  • J. Carballido
Original Paper


Metabolic syndrome (MS) individuals have a higher risk of developing chronic kidney disease through unclear pathogenic mechanisms. MS has been also related with higher nephrolithiasis prevalence. To establish the influence of MS on renal function, we designed a murine model of combined metabolic syndrome and hyperoxaluria. Four groups of male Sprague–Dawley rats were established: (1) control group (n = 10) fed with standard chow; (2) stone former group (SF) (n = 10) fed with standard chow plus 0.75% ethylene glycol administered in the drinking water; (3) metabolic syndrome group (MS) (n = 10), fed with 60% fructose diet; (4) metabolic syndrome + stone former group (MS + SF) (n = 10), 60% fructose diet and 0.75% EG in the drinking water. MS group showed a significant injury to renal function when hyperoxaluria was induced. It was demonstrated by a significant decrease of creatinine clearance (p < 0.001), with higher tubular damage (34.3%, CI 95% 23.9–44.7, p < 0.001), produced by deposition of crystals, and increased tubular synthesis of osteopontin as a response to tubular damage. Induction of hyperoxaluria in rats with MS causes severe morphological alterations with a significant impairment of renal function. This impairment is not produced in rats without MS. Therefore, this model can be useful for the study of the influence of MS in stone formation.


Metabolic syndrome Oxidative stress Renal stone Hyperoxaluria 



The Urological Society from Madrid, SUM Research Grant 2013 and Astellas Pharma España are acknowledged.

Compliance with ethical standards


Urological Society from Madrid. Research Grant 2013 and Astellas Pharma España are acknowledged.

Conflict of interest

Sáenz-Medina J., Jorge E., Corbacho C., Santos M., Sánchez A., Soblechero P., Virumbrales E., Ramil E., Coronado M.J., Castillón I., Carballido J. declare that they have no conflict of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  • Javier Sáenz-Medina
    • 1
    Email author
  • E. Jorge
    • 2
  • C. Corbacho
    • 3
  • M. Santos
    • 4
  • A. Sánchez
    • 5
  • P. Soblechero
    • 2
  • E. Virumbrales
    • 2
  • E. Ramil
    • 6
  • M. J. Coronado
    • 7
  • I. Castillón
    • 1
  • D. Prieto
    • 8
  • J. Carballido
    • 1
  1. 1.Department of UrologyHospital Universitario Puerta de Hierro-MajadahondaMadridSpain
  2. 2.Department of Clinical BiochemistryHospital Universitario Puerta de Hierro-MajadahondaMadridSpain
  3. 3.Department of PathologyHospital Universitario Puerta de Hierro-MajadahondaMadridSpain
  4. 4.Medical and Surgical Research FacilityInstituto de Investigación Sanitaria Puerta de HierroMadridSpain
  5. 5.Biobank, Instituto de Investigación Sanitaria Puerta de HierroMadridSpain
  6. 6.Molecular Biology and DNA Sequencing FacilityInstituto de Investigación Sanitaria Puerta de HierroMadridSpain
  7. 7.Confocal Microscopy FacilityInstituto de Investigación Sanitaria Puerta de HierroMadridSpain
  8. 8.Department of Animal Phisiology, Facultad de FarmaciaUniversidad Complutense de MadridMadridSpain

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